Markers of type I and type III collagen synthesis in serum as indicators of tissue growth during pregnancy

1993 ◽  
Vol 77 (1) ◽  
pp. 178-182 ◽  
Author(s):  
U. Puistola
Keyword(s):  
Collagen Synthesis ◽  
Tissue Growth ◽  
Type Iii Collagen ◽  
Type I ◽  
Type Iii

Change in phenotype in long-term cultures of a clonal rat bone cell line: switch to the synthesis of α1 (I)-trimer collagen

1984 ◽  
Vol 62 (6) ◽  
pp. 462-469 ◽  
Author(s):  
Hardy Limeback ◽  
Kichibee Otsuka ◽  
Kam-Ling Yao ◽  
Jane E. Aubin ◽  
Jaro Sodek
Keyword(s):  
Collagen Synthesis ◽  
Type I Collagen ◽  
Bone Cell ◽  
Detectable Effect ◽  
Prostaglandin E ◽  
Intracellular Camp ◽  
Type Iii Collagen ◽  
Type I ◽  
Type Iii ◽  
Type V

A number of bone cell clones isolated from rat calvaria have been maintained in culture for more than 3 years. Several of these clones have undergone dramatic changes in phenotype. One of these clones, RGB 2.2, was observed originally to have a fibroblastic morphology in culture and to respond to parathyroid hormone (PTH), but not prostaglandin E2 (PGE2), with an increase in intracellular cAMP. Throughout several passages in early subcultures, these cells synthesized mostly type I collagen, with small amounts of type III and type V collagens. Whereas PTH had no detectable effect on collagen synthesis, PGE2 decreased the amount of total cell layer collagen, with the greatest effect on type III collagen, while increasing the proportion of type V collagen. Subsequent studies on these cells during 3 years in culture have indicated changes in their phenotype including a progressive change in morphology to a more cuboidal shape and a change in collagen synthesis, the cells producing large amounts of the "embryonic" collagen, α1(I) trimer. The reason(s) for the change in collagen expression is unknown, but may be the result of a change in which gene(s) is being expressed.

scholarly journals Asiaticoside induces cell proliferation and collagen synthesis in human dermal fibroblasts

2015 ◽  
Vol 34 (2) ◽  
pp. 96
Author(s):  
Linda Yuliati ◽  
Etik Mardliyati ◽  
Kusmarinah Bramono ◽  
Hans Joachim Freisleben
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Retinoic Acid ◽  
Collagen Synthesis ◽  
Active Agent ◽  
Dermal Fibroblasts ◽  
Type Iii Collagen ◽  
Type I ◽  
Human Dermal Fibroblasts ◽  
Type Iii

Background<br />Asiatiocoside, a saponin component isolated from Centella asiatica can improve wound healing by promoting the proliferation of human dermal fibroblasts (HDF) and synthesis of collagen. The skin-renewing cells and type I and III collagen synthesis decrease with aging, resulting in the reduction of skin elasticity and delayed wound healing. Usage of natural active compounds from plants in wound healing should be evaluated and compared to retinoic acid as an active agent that regulates wound healing. The aim of this study was to compare and evaluate the effect of asiaticoside and retinoic acid to induce greater cell proliferation and type I and III collagen synthesis in human dermal fibroblast.<br /><br />Methods<br />Laboratory experiments were conducted using human dermal fibroblasts (HDF) isolated from human foreskin explants. Seven passages of HDF were treated with asiaticoside and retinoic acid at several doses and incubated for 24 and 48 hours. Cell viability in all groups was tested with the MTT assay to assess HDF proliferation. Type I and III collagen synthesis was examined using the respective ELISA kits. Analysis of variance was performed to compare the treatment groups. <br /><br />Results<br />Asiaticoside had significantly stronger effects on HDF proliferation than retinoic acid (p&lt;0.05). The type III collagen production was significantly greater induction with asiaticoside compared to retinoic acid (p&lt;0.05). <br /><br />Conclusion<br />Asiaticoside induces HDF proliferation and type I and III collagen synthesis in a time- and dose-dependent pattern. Asiaticoside has a similar effect as retinoic acid on type I and type III collagen synthesis.

scholarly journals Metabolism of rabbit skin collagen. Differences in the apparent turnover rates of type-I- and type-III-collagen precursors determined by constant intravenous infusion of labelled amino acids

1979 ◽  
Vol 181 (1) ◽  
pp. 75-82 ◽  
Author(s):  
S P Robins
Keyword(s):  
Amino Acid ◽  
Collagen Synthesis ◽  
Specific Radioactivity ◽  
Deae Cellulose ◽  
Type Iii Collagen ◽  
Type I ◽  
Turnover Rates ◽  
Type Iii ◽  
Type I Procollagen ◽  
Skin Collagen

Growing rabbits were infused for up to 10 h with labelled proline, tyrosine and leucine to achieve plateau conditions within body free pools, for [3H]proline infusion, blood free-proline specific radioactivity remained constant after about 1 h. For individual animals, type-I- and type-III-collagen precursors were isolated by precipitation with (NH4)2SO4 and DEAE-cellulose chromatography. Experiments where 3H- and 14C-labelled proline and tyrosine were infused concurrently for different periods of time showed that type I procollagen reached plateau specific radioactivity within 3 h and 90% of the plateau value after 2 h infusion, corresponding to a calculated apparent t 1/2 of less than 26 min. Plateau values for type I procollagen were taken as precursor amino acid pool specific radioactivities. The type-III-collagen-precursor fractions consistently showed lower rates of label incorporation and, by assuming that both type I and type III collagens are synthesized from the same amino acid pools, kinetic analysis revealed an apparent t 1/2 for the isolated type-III-collagen precursors of 3.9 h. For proline, there were large variations between animals in the ratio between the precursor pool for collagen synthesis and the skin homogenate free pool (0.31 +/- 0.13, mean +/- S.D.), so that collagen-synthesis rates based solely on total tissue free-pool values for proline are subject to large and inconsistent errors.

Effects of UVA irradiation following treatment with 8-methoxypsoralen on type I and type III collagen synthesis in normal and scleroderma fibroblast cultures

2009 ◽  
Vol 301 (7) ◽  
pp. 507-513 ◽  
Author(s):  
Yohei Kitamura ◽  
Hiromi Namikawa ◽  
Shujiro Hayashi ◽  
Takahiro Yoshida ◽  
Toshihiro Suzuki ◽  
...  
Keyword(s):  
Collagen Synthesis ◽  
Type Iii Collagen ◽  
Type I ◽  
Type Iii ◽  
Uva Irradiation ◽  
Fibroblast Cultures ◽  
Scleroderma Fibroblast

Type I and type III collagen synthesis and composition in the valve matrix in aortic valve stenosis

2006 ◽  
Vol 189 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Heidi A. Eriksen ◽  
Jari Satta ◽  
Juha Risteli ◽  
Mikko Veijola ◽  
Päivi Väre ◽  
...  
Keyword(s):  
Aortic Valve ◽  
Aortic Valve Stenosis ◽  
Collagen Synthesis ◽  
Type Iii Collagen ◽  
Type I ◽  
Type Iii

Interleukin-4 modulates collagen synthesis by human mesangial cells in a type-specific manner

1996 ◽  
Vol 270 (3) ◽  
pp. F447-F453 ◽  
Author(s):  
Y. Nakazato ◽  
H. Okada ◽  
S. Tajima ◽  
T. Hayashida ◽  
Y. Kanno ◽  
...  
Keyword(s):  
Collagen Synthesis ◽  
Mesangial Cells ◽  
Interleukin 4 ◽  
Northern Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type Iii Collagen ◽  
Type I ◽  
Glomerular Diseases ◽  
Human Mesangial Cells ◽  
Type Iii

Several studies have suggested an involvement of interleukin (IL)-4 in the pathophysiology of glomerulonephritis (GN). To elucidate its possible role in GN, we have investigated the effect of IL-4 on collagen accumulation by human mesangial cells (MC). After incubation with IL-1 alpha and/or IL-4 for 48 h, type I, III, and IV collagens in both soluble and cell-associated forms were identified by Western blotting. IL-1 alpha stimulated type I and IV collagen synthesis, lacking significant effect on type III collagen synthesis. In contrast, IL-4 stimulated type III collagen synthesis without affecting type I and type IV synthesis. Enzyme-linked immunosorbent assay confirmed the dose-dependent effect of IL-4 on collagen type III secretion (2.0-fold at 10 ng/ml). Importantly, IL-1 alpha-stimulated type I and IV collagen synthesis was suppressed by concomitant IL-4 treatment. Northern analysis of type I and III procollagen mRNAs displayed consistent results. These data indicate that IL-4 selectively stimulates type III collagen synthesis but also suppresses IL-1 alpha-stimulated type I and IV collagen synthesis. Therefore IL-4 could potentially contribute to the pathological changes in glomerular diseases in cooperate with other cytokines.

Responses of Type I and Type III Collagen Synthesis after Replacement of the Hip Joint

1995 ◽  
Vol 58 (5) ◽  
pp. 443-448
Author(s):  
Kari Haukipuro ◽  
Leila Risteli ◽  
Juhana Leppilahti ◽  
Jukka Melkko ◽  
Jaakko Puranen ◽  
...  
Keyword(s):  
Hip Joint ◽  
Collagen Synthesis ◽  
Type Iii Collagen ◽  
Type I ◽  
Type Iii
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