Immunohistochemical localization of 3 beta-hydroxy-5-ene-steroid dehydrogenase/delta 5----delta 4 isomerase in human placenta and fetal membranes throughout gestation

1992 ◽  
Vol 75 (3) ◽  
pp. 956-961 ◽  
Author(s):  
S. C. Riley
Keyword(s):  
Human Placenta ◽  
Immunohistochemical Localization ◽  
Fetal Membranes ◽  
Steroid Dehydrogenase

Changes in the Abundance of mRIMA for Type-l 3β-Hydroxy-steroid Dehydrogenase/Δ5→Δ4Isomerase in the Human Placenta and Fetal Membranes during Pregnancy and Labor

1993 ◽  
Vol 35 (4) ◽  
pp. 199-203 ◽  
Author(s):  
Simon C. Riley ◽  
Nicole S. Bassett ◽  
Edward T.M. Berdusco ◽  
Kaiping Yang ◽  
Cheryl Leystra-Lantz ◽  
...  
Keyword(s):  
Human Placenta ◽  
Fetal Membranes ◽  
Hydroxy Steroid ◽  
Steroid Dehydrogenase

scholarly journals Immunohistochemical Localization of Carboxypeptidases E and D in the Human Placenta and Umbilical Cord

1998 ◽  
Vol 46 (12) ◽  
pp. 1359-1367 ◽  
Author(s):  
Sandra E. Reznik ◽  
Carolyn M. Salafia ◽  
Janice M. Lage ◽  
Lloyd D. Fricker
Keyword(s):  
Umbilical Cord ◽  
Human Placenta ◽  
Immunohistochemical Localization

Labour-associated changes in adrenomedullin content in human placenta and fetal membranes

2003 ◽  
Vol 105 (4) ◽  
pp. 419-423 ◽  
Author(s):  
A. AL-GHAFRA ◽  
N. M. GUDE ◽  
S. P. BRENNECKE ◽  
R. G. KING
Keyword(s):  
Human Placenta ◽  
Mode Of Delivery ◽  
Placental Tissue ◽  
Fetal Membrane ◽  
Fetal Membranes ◽  
Tissue Extracts ◽  
Human Labour ◽  
Labour Group

The aim of the present study was to determine the effects of labour and mode of delivery on human placental and fetal membrane content of adrenomedullin (AdM). Placentas and fetal membranes were collected either at term or pre-term gestation from women either in labour or not in labour, and AdM was measured in tissue extracts by specific RIA. There were significant increases in AdM concentrations in amnion and choriodecidua for the in-labour group compared with the not-in-labour group for both pre-term and term gestations. There was no difference in AdM concentration in placental tissue between labour groups. This study provides evidence that fetal membrane AdM is increased in amniotic and choriodecidual tissues in response to labour, and suggests that it may play a role during human labour.

Metallothionein-like proteins in human placenta and fetal membranes

1984 ◽  
Vol 74 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Michael P. Waalkes ◽  
Alan M. Poisner ◽  
Gary W. Wood ◽  
Curtis D. Klaassen
Keyword(s):  
Human Placenta ◽  
Fetal Membranes

scholarly journals Mechanistic and stereochemical studies on 3-oxo steroid Δ4-Δ5-isomerase from human placenta

1980 ◽  
Vol 185 (2) ◽  
pp. 411-421 ◽  
Author(s):  
M Akhtar ◽  
M Calder ◽  
T Smith ◽  
J N Wright
Keyword(s):  
Double Bond ◽  
Hydrogen Atom ◽  
Human Placenta ◽  
Microsomal Fraction ◽  
Isomerization Reaction ◽  
Acidic Group ◽  
Incoming Proton ◽  
Membrane Bound ◽  
Hydroxy Steroid ◽  
Steroid Dehydrogenase

The mechanism of isomerization of delta 5-3-ox steroids to delta 4-3-oxo steroids was examined by using the membrane-bound 3-oxo steroid delta 4-delta 5-isomerase (EC 5.3.3.1) and the 3 beta-hydroxy steroid dehydrogenase present in the microsomal fraction obtained from full-term human placenta. (1) Methods for the preparation of androst-5-ene-3 beta, 17 beta-diol specifically labelled at the 4 alpha-, 4 beta- or 6-positions are described. (2) Incubations with androst-5-ene-3 beta, 17 beta-diol stereospecifically 3H-labelled either in the 4 alpha- or 4 beta-position showed that the isomerization reaction occurs via a stereospecific elimination of the 4 beta hydrogen atom. In addition, the complete retention of 3H in the delta 4-3-oxo steroids obtained from [4 alpha-3H]androst-5-ene-3 beta, 17 beta-diol indicates that the non-enzymic contribution to these experiments was negligible. (3) To study the stereochemistry of the insertion of the incoming proton at C-6, the [6-3H]androst-4-ene-3, 17-dione obtained from the oxidation isomerization of [6-3H]androst-5-ene-3 beta, 17 beta-diol was enzymically hydroxylated in the 6 beta-position by the fungus Rhizopls stolonifer. Retention of 3H in the 6 alpha-position of the isolated 6 beta-hydroxyandrost-4-ene-3, 17-dione indicates that in the isomerase-catalysed migration of the C(5) = C(6) double bond, the incoming proton from the acidic group on the enzyme must enter C-6 from the beta-face, forcing the existing 3H into the 6 alpha-position.

Human decidual and placental relaxins

1991 ◽  
Vol 3 (4) ◽  
pp. 385 ◽  
Author(s):  
GD Bryant-Greenwood
Keyword(s):  
Extracellular Matrix ◽  
Corpus Luteum ◽  
Human Placenta ◽  
Northern Analysis ◽  
Fetal Membranes ◽  
Decidual Cell ◽  
Matrix Interaction ◽  
Polypeptide Hormones ◽  
Collagen Remodelling

The human placenta and decidua are intrauterine production sites for a range of polypeptide hormones. Relaxin is one of these hormones, its production having been demonstrated by immunocytochemistry and Northern analysis. There are two relaxin genes in the human genome, termed H1 and H2; only the latter is expressed in cyclic and pregnant corpus luteum. However, it has recently been shown that both genes are expressed in the decidua and placenta. It is not known whether both are translated. These hormone(s) may act in a paracrine fashion and be partly responsible for the control of enzymes and inhibitors involved in collagen remodelling in the fetal membranes in the last weeks of pregnancy. An autocrine role of decidual relaxin and a possible decidual-cell/macrophage/extracellular-matrix interaction is described; this may act as a unit in the elaboration of a range of hormones.

Immunohistochemical Localization of ECE-1 in the Human Placenta

Placenta ◽  
2000 ◽  
Vol 21 (2-3) ◽  
pp. 226-233 ◽  
Author(s):  
Z. Ahmad ◽  
S.E. Reznik
Keyword(s):  
Human Placenta ◽  
Immunohistochemical Localization
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