scholarly journals Advances in Understanding Autoimmune Pituitary Disease: Standardized Methods for Autoantibody Detection

2014 ◽  
Vol 99 (5) ◽  
pp. 1589-1592
Author(s):  
Terry J. Smith
Keyword(s):  
Pituitary Disease ◽  
Autoantibody Detection

scholarly journals Proton-Beam Therapy of Pituitary Disease

1974 ◽  
Vol 67 (1) ◽  
pp. 35-36 ◽  
Author(s):  
M J Francis ◽  
Julia P Ellis ◽  
T D R Hockaday
Keyword(s):  
Proton Beam ◽  
Proton Beam Therapy ◽  
Pituitary Disease

Glycopeptide microarray for autoantibody detection in cancer

2011 ◽  
Vol 8 (4) ◽  
pp. 435-437 ◽  
Author(s):  
Celso A Reis ◽  
Diana Campos ◽  
Hugo Osório ◽  
Lúcio Lara Santos
Keyword(s):  
Autoantibody Detection

scholarly journals Expanding Assay Dynamics: A Combined Competitive and Direct Assay System for the Quantification of Proteins in Multiplexed Immunoassays

2008 ◽  
Vol 54 (6) ◽  
pp. 956-963 ◽  
Author(s):  
Michael Hartmann ◽  
Monika Schrenk ◽  
Anette Döttinger ◽  
Sarah Nagel ◽  
Johan Roeraade ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Protein Microarray ◽  
Measurement Range ◽  
Multiplex Assay ◽  
Competitive System ◽  
Direct Immunoassay ◽  
Sandwich Configuration ◽  
Readout Channel ◽  
Autoantibody Detection ◽  
Detection And Quantification

Abstract Background: The concurrent detection and quantification of analytes that vary widely in concentration present a principal problem in multiplexed assay systems. Combining competitive and sandwich immunoassays permits coverage of a wide concentration range, and both highly abundant molecules and analytes present in low concentration can be quantified within the same assay. Methods: The use of different fluorescence readout channels allows the parallel use of a competitive system and a sandwich configuration. The 2 generated assay signals are combined and used to calculate the amount of analyte. The measurement range can be adjusted by varying the competitor concentration, and an extension of the assay system’s dynamic range is possible. Results: We implemented the method in a planar protein microarray–based autoimmune assay to detect autoantibodies against 13 autoantigens and to measure the concentration of a highly abundant protein, total human IgG, in one assay. Our results for autoantibody detection and IgG quantification agreed with results obtained with commercially available assays. The use of 2 readout channels in the protein microarray–based system reduced spot-to-spot variation and intraassay variation. Conclusions: By combining a direct immunoassay with a competitive system, analytes present in widely varying concentrations can be quantified within a single multiplex assay. Introducing a second readout channel for analyte quantification is an effective tool for spot-to-spot normalization and helps to lower intraassay variation.

The perspective of patients with pituitary disease on work according to the expanded ICF model: A qualitative study

2021 ◽  
Author(s):  
Van der Meulen Merel ◽  
Daniel J. Lobatto ◽  
R. van Furth Wouter ◽  
Sasja D. Huisman ◽  
Yvonne F. Heerkens ◽  
...  
Keyword(s):  
Qualitative Study ◽  
Pituitary Disease ◽  
Icf Model
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