scholarly journals Acquired Obesity Increases CD68 and Tumor Necrosis Factor-α and Decreases Adiponectin Gene Expression in Adipose Tissue: A Study in Monozygotic Twins

2006 ◽  
Vol 91 (7) ◽  
pp. 2776-2781 ◽  
Author(s):  
Kirsi H. Pietiläinen ◽  
Katja Kannisto ◽  
Elena Korsheninnikova ◽  
Aila Rissanen ◽  
Jaakko Kaprio ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Monozygotic Twins ◽  
Adiponectin Gene ◽  
Factor Α ◽  
Necrosis Factor

Insulin stimulates interleukin-6 and tumor necrosis factor-α gene expression in human subcutaneous adipose tissue

2004 ◽  
Vol 286 (2) ◽  
pp. E234-E238 ◽  
Author(s):  
Rikke Krogh-Madsen ◽  
Peter Plomgaard ◽  
Pernille Keller ◽  
Charlotte Keller ◽  
Bente Klarlund Pedersen
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Tumor Necrosis Factor ◽  
Skeletal Muscles ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

High circulating levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) are found in patients with hyperinsulinemia. Insulin stimulates release of IL-6 from adipocyte cultures, and it stimulates IL-6 gene expression in insulin-resistant, but not control, rat skeletal muscle. In addition, TNF-α may be involved in the pathogenesis of insulin resistance. Therefore, we studied the effect of insulin on IL-6 and TNF-α gene expression in human skeletal muscle and adipose tissue. Nine healthy young volunteers participated in the study. They underwent a 6-h hyperinsulinemic euglycemic clamp at a fixed insulin infusion rate, with blood glucose clamped at fasting level. Blood samples drawn at 0, 1, 2, 3, 4, 5, and 6 h were analyzed for IL-6 and TNF-α. Muscle and fat biopsies, obtained at 0, 2, 4, and 6 h, were analyzed for IL-6 and TNF-α mRNA with real-time PCR. IL-6 mRNA increased 11-, 3-, and 5-fold at 2, 4, and 6 h, respectively, in adipose tissue (ANOVA P = 0.027), whereas there was no significant effect of insulin on skeletal muscles. Plasma IL-6 increased during insulin stimulation. TNF-α mRNA increased 2.4-, 1.4-, and 2.2-fold in adipose tissue (ANOVA P = 0.001) and decreased 0.74-, 0.64-, and 0.68-fold in muscle tissue (ANOVA P = 0.04). Plasma levels of TNF-α were constant. In conclusion, the finding that insulin stimulates IL-6 and TNF-α gene expression in adipose tissue only and inhibits the TNF-α production in skeletal muscles suggests a differential regulation of muscle- and adipose tissue-derived IL-6 and TNF-α.

Aspirin Inhibits Tumor Necrosis Factor-α Gene Expression in Murine Tissue Macrophages

1997 ◽  
Vol 52 (3) ◽  
pp. 421-429 ◽  
Author(s):  
Rodney E. Shackelford ◽  
Paul B. Alford ◽  
Yan Xue ◽  
Sheau-Fung Thai ◽  
Dolph O. Adams ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Murine Tissue ◽  
Factor Α ◽  
Necrosis Factor
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