scholarly journals Glucose Transport and Phosphorylation in Skeletal Muscle in Obesity: Insight from a Muscle-Specific Positron Emission Tomography Model

2003 ◽  
Vol 88 (3) ◽  
pp. 1271-1279 ◽  
Author(s):  
Katherine V. Williams ◽  
Alessandra Bertoldo ◽  
Bruno Mattioni ◽  
Julie C. Price ◽  
Claudio Cobelli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Glucose Transport ◽  
Emission Tomography ◽  
Positron Emission

scholarly journals Quantitative Assessment of Glucose Transport in Human Skeletal Muscle: Dynamic Positron Emission Tomography Imaging of [O-Methyl-11C]3-O-Methyl-d-Glucose

2005 ◽  
Vol 90 (3) ◽  
pp. 1752-1759 ◽  
Author(s):  
Alessandra Bertoldo ◽  
Julie Price ◽  
Chet Mathis ◽  
Scott Mason ◽  
Daniel Holt ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Glucose Transport ◽  
Pet Imaging ◽  
Human Skeletal Muscle ◽  
Insulin Infusion ◽  
Emission Tomography ◽  
Dynamic Pet ◽  
Positron Emission ◽  
18F Fdg

Insulin-stimulated glucose transport in skeletal muscle is regarded as a key determinant of insulin sensitivity, yet isolation of this step for quantification in human studies is a methodological challenge. One notable approach is physiological modeling of dynamic positron emission tomography (PET) imaging using 2-[18-fluoro]2-deoxyglucose ([18F]FDG); however, this has a potential limitation in that deoxyglucose undergoes phosphorylation subsequent to transport, complicating separate estimations of these steps. In the current study we explored the use of dynamic PET imaging of [11C]3-O-methylglucose ([11C]3-OMG), a glucose analog that is limited to bidirectional glucose transport. Seventeen lean healthy volunteers with normal insulin sensitivity participated; eight had imaging during basal conditions, and nine had imaging during euglycemic insulin infusion at 30 mU/min·m2. Dynamic PET imaging of calf muscles was conducted for 90 min after the injection of [11C]3-OMG. Spectral analysis of tissue activity indicated that a model configuration of two reversible compartments gave the strongest statistical fit to the kinetic pattern. Accordingly, and consistent with the structure of a model previously used for [18F]FDG, a two-compartment model was applied. Consistent with prior [18F]FDG findings, insulin was found to have minimal effect on the rate constant for movement of [11C]3-OMG from plasma to tissue interstitium. However, during insulin infusion, a robust and highly significant increase was observed in the kinetics of inward glucose transport; this and the estimated tissue distribution volume for [11C]3-OMG increased 6-fold compared with basal conditions. We conclude that dynamic PET imaging of [11C]3-OMG offers a novel quantitative approach that is both chemically specific and tissue specific for in vivo assessment of glucose transport in human skeletal muscle.

scholarly journals Exercise Restores Skeletal Muscle Glucose Delivery But Not Insulin-Mediated Glucose Transport and Phosphorylation in Obese Subjects

2006 ◽  
Vol 91 (9) ◽  
pp. 3394-3403 ◽  
Author(s):  
L. Slimani ◽  
V. Oikonen ◽  
K. Hällsten ◽  
N. Savisto ◽  
J. Knuuti ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Glucose Transport ◽  
Oxidative Capacity ◽  
Emission Tomography ◽  
Glucose Disposal ◽  
Oxidative Potential ◽  
Positron Emission ◽  
Significant Difference ◽  
Obese Subjects

Abstract Context/Objective: Insulin resistance in obese subjects results in the impaired disposal of glucose by skeletal muscle. The current study examined the effects of insulin and/or exercise on glucose transport and phosphorylation in skeletal muscle and the influence of obesity on these processes. Subjects/Methods: Seven obese and 12 lean men underwent positron emission tomography with 2-deoxy-2-[18F]fluoro-d-glucose in resting and isometrically exercising skeletal muscle during normoglycemic hyperinsulinemia. Data were analyzed by two-tissue compartmental modeling. Perfusion and oxidative capacity were measured during insulin stimulation by [15O]H2O and [15O]O2. Results: Exercise increased glucose fractional uptake (K), inward transport rate (K1), and the k3 parameter, combining transport and intracellular phosphorylation, in lean and obese subjects. In each group, there was no statistically significant difference between plasma flow and K1. At rest, a significant defect in K1 (P = 0.0016), k3 (P = 0.016), and K (P = 0.022) was found in obese subjects. Exercise restored K1, improved but did not normalize K (P = 0.03 vs. lean), and did not ameliorate the more than 60% relative impairment in k3 in obese individuals (P = 0.002 vs. lean). The glucose oxidative potential tended to be reduced by obesity. Conclusions/Interpretation: The study indicates that exercise restores the impairment in insulin-mediated skeletal muscle perfusion and glucose delivery associated with obesity but does not normalize the defect involving the proximal steps regulating glucose disposal in obese individuals. Our data support the use of 2-deoxy-2-[18F]fluoro-d-glucose-positron emission tomography in the dissection between substrate supply and intrinsic tissue metabolism.

Evaluation of individual skeletal muscle activity by glucose uptake during pedaling exercise at different workloads using positron emission tomography

2009 ◽  
Vol 107 (2) ◽  
pp. 599-604 ◽  
Author(s):  
Yuichi Gondoh ◽  
Manabu Tashiro ◽  
Masatoshi Itoh ◽  
Mohammad M. Masud ◽  
Hiroomi Sensui ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Glucose Uptake ◽  
Muscle Activity ◽  
Rectus Femoris ◽  
Emission Tomography ◽  
Whole Body ◽  
Control Subjects ◽  
Positron Emission ◽  
Iliacus Muscle

Skeletal muscle glucose uptake closely reflects muscle activity at exercise intensity levels <55% of maximal oxygen consumption (V̇o2max). Our purpose was to evaluate individual skeletal muscle activity from glucose uptake in humans during pedaling exercise at different workloads by using [18F]fluorodeoxyglucose (FDG) and positron emission tomography (PET). Twenty healthy male subjects were divided into two groups (7 exercise subjects and 13 control subjects). Exercise subjects were studied during 35 min of pedaling exercise at 40 and 55% V̇o2max exercise intensities. FDG was injected 10 min after the start of exercise or after 20 min of rest. PET scanning of the whole body was conducted after completion of the exercise or rest period. In exercise subjects, mean FDG uptake [standardized uptake ratio (SUR)] of the iliacus muscle and muscles of the anterior part of the thigh was significantly greater than uptake in muscles of control subjects. At 55% V̇o2max exercise, SURs of the iliacus muscle and thigh muscles, except for the rectus femoris, increased significantly compared with SURs at 40% V̇o2max exercise. Our results are the first to clarify that the iliacus muscle, as well as the muscles of the anterior thigh, is the prime muscle used during pedaling exercise. In addition, the iliacus muscle and all muscles in the thigh, except for the rectus femoris, contribute when the workload of the pedaling exercise increases from 40 to 55% V̇o2max.

scholarly journals Evaluation of Skeletal Muscle Activity During Foot Training Exercises Using Positron Emission Tomography

2021 ◽  
Author(s):  
Tomoyuki Kanayama ◽  
Junsuke Nakase ◽  
Takafumi Mochizuki ◽  
Kazuki Asai ◽  
Rikuto Yoshimizu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Muscle Activity ◽  
Skeletal Muscles ◽  
Standardized Uptake Value ◽  
Emission Tomography ◽  
Abductor Hallucis ◽  
Continuous Training ◽  
Positron Emission ◽  
The Difference

Abstract The foot exercises “rock-paper-scissors” and “towel gathering” are widely used in patients with lower limb disorders; however, there are no detailed reports on muscle activity during such training. We quantitatively evaluated the difference in skeletal muscle activity between the two exercises using positron emission tomography. Eight university student athletes were included. Four participants each were assigned to the foot rock-paper-scissors and towel gathering groups. Participants in each group underwent continuous training for 15 min. They received an intravenous injection of 18F-fluorodeoxyglucose and retrained for 15 min, following which they rested for 45 min. Regions of interest were defined in 25 muscles. The standardized uptake value (SUV) in the trained limb was compared with that in the non-trained control limb. SUVs increased in four skeletal muscles (tibialis anterior, peroneus brevis, extensor hallucis brevis, and abductor hallucis) in the rock-paper-scissors group, and in four muscles (flexor digitorum longus, extensor hallucis brevis, extensor digitorum brevis, and quadratus plantae) in the towel gathering group. Thus, foot rock-paper-scissors and towel gathering affected skeletal muscles related to the medial longitudinal arch and toe grip strength, respectively. Given that the two exercises target different skeletal muscles, they should be taught and implemented according to their respective purposes.

Potential of [11C]TMSX for the evaluation of adenosine A2A receptors in the skeletal muscle by positron emission tomography

2004 ◽  
Vol 31 (7) ◽  
pp. 949-956 ◽  
Author(s):  
Kiichi Ishiwata ◽  
Masaki Mizuno ◽  
Yuichi Kimura ◽  
Kazunori Kawamura ◽  
Keiichi Oda ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Emission Tomography ◽  
Adenosine A2a Receptors ◽  
A2a Receptors ◽  
Positron Emission ◽  
Adenosine A2a

Assessment of Skeletal Muscle Ventricle Tissue Blood Flow Using Positron Emission Tomography

2001 ◽  
Vol 25 (4) ◽  
pp. 306-312
Author(s):  
Tajinder P. Singh ◽  
Kevin Greer ◽  
Otto Muzik ◽  
Robert L. Hammond ◽  
Larry W. Stephenson ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Blood Flow ◽  
Emission Tomography ◽  
Tissue Blood Flow ◽  
Positron Emission

Assessing Skeletal Muscle Glucose Metabolism With Positron Emission Tomography

IUBMB Life ◽  
2001 ◽  
Vol 52 (6) ◽  
pp. 279-284 ◽  
Author(s):  
David E. Kelley ◽  
Julie C. Price ◽  
Claudio Cobelli
Keyword(s):  
Skeletal Muscle ◽  
Positron Emission Tomography ◽  
Glucose Metabolism ◽  
Emission Tomography ◽  
Positron Emission
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