scholarly journals Social Status Predicts How Sex Steroid Receptors Regulate Complex Behavior across Levels of Biological Organization

Endocrinology ◽  
2012 ◽  
Vol 153 (3) ◽  
pp. 1341-1351 ◽  
Author(s):  
Lauren A. O'Connell ◽  
Hans A. Hofmann
Keyword(s):  
Gene Expression ◽  
Receptor Antagonist ◽  
Social Status ◽  
Steroid Receptors ◽  
Courtship Behavior ◽  
Receptor Gene ◽  
Steroid Receptor ◽  
Gonadal Hormones ◽  
Sex Steroid ◽  
Biological Organization

Social status strongly affects behavior and physiology, in part mediated by gonadal hormones, although how each sex steroid acts across levels of biological organization is not well understood. We examine the role of sex steroids in modulating social behavior in dominant (DOM) and subordinate (SUB) males of a highly social fish, Astatotilapia burtoni. We first used agonists and antagonists to each sex steroid receptor and found that androgens and progestins modulate courtship behavior only in DOM, whereas estrogens modulate aggressive behavior independent of social status. We then examined the hormonal and physiological responses to sex steroid receptor antagonist treatment and uncovered substantial changes in circulating steroid hormone levels and gonad size only in SUB, not in DOM. Consistent with status-based physiological sensitivities to drug manipulation, we found that neuropeptide and steroid receptor gene expression in the preoptic area was sensitive only in SUB. However, when we compared the transcriptomes of males that received either vehicle or an estrogen receptor antagonist, 8.25% of all genes examined changed expression in DOM in comparison with only 0.56% in SUB. Finally, we integrate behavior, physiology, and brain gene expression to infer functional modules that underlie steroid receptor regulation of behavior. Our work suggests that environmentally induced changes at one level of biological organization do not simply affect changes of similar magnitude at other levels, but that instead very few key pathways likely serve as conduits for executing plastic responses across multiple levels.

Molecular study of sex steroid receptor gene expression in human colon and in colorectal carcinomas

1997 ◽  
Vol 64 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Przemyslaw Waliszewski ◽  
Miroslawa Blaszczyk ◽  
Ewa Wolinska-Witort ◽  
Michal Drews ◽  
Marek Snochowski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Human Colon ◽  
Steroid Receptor ◽  
Molecular Study ◽  
Sex Steroid ◽  
Colorectal Carcinomas ◽  
Receptor Gene Expression

Interleukin-1 System and Sex Steroid Receptor Gene Expression in Human Endometrial Cancer

2002 ◽  
Vol 85 (3) ◽  
pp. 423-430 ◽  
Author(s):  
C.F. Singer ◽  
N. Kronsteiner ◽  
E. Marton ◽  
I. Walter ◽  
M. Kubista ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endometrial Cancer ◽  
Receptor Gene ◽  
Interleukin 1 ◽  
Steroid Receptor ◽  
Sex Steroid ◽  
Receptor Gene Expression

Uterine myoma and adenomyosis: molecular characterization by steroid receptor gene expression

2018 ◽  
Vol 4_2018 ◽  
pp. 58-63
Author(s):  
Shramko S.V. Shramko ◽  
Gulyaeva L.F. Gulyaeva ◽  
Bazhenova L.G. Bazhenova ◽  
Levchenko V.G. Levchenko ◽  
◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Characterization ◽  
Receptor Gene ◽  
Steroid Receptor ◽  
Uterine Myoma ◽  
Receptor Gene Expression

scholarly journals Recent insights into the origins of adrenal and sex steroid receptors

2002 ◽  
Vol 28 (3) ◽  
pp. 149-152 ◽  
Author(s):  
ME Baker
Keyword(s):  
Estrogen Receptor ◽  
Progesterone Receptor ◽  
Gene Transcription ◽  
Steroid Receptors ◽  
Alternative Hypothesis ◽  
Steroid Receptor ◽  
Sex Steroid ◽  
Sex Steroid Receptors ◽  
Receptor Phylogeny ◽  
Corticoid Receptor

The recent cloning by Thornton (2001) of estrogen, progesterone and corticoid receptors from lamprey provides important insights into the early evolution of adrenal and sex steroid receptors and an opportunity to elucidate the ancient steroids that regulated gene transcription. Inclusion of lamprey sequences in a steroid receptor phylogeny indicates that the estrogen receptor is the most ancient of these receptors, followed by the progesterone receptor and the corticoid receptor. Thornton proposed that estradiol was the earliest of the steroids to activate a steroid receptor. An alternative hypothesis is that a steroid in the Delta(5) pathway activated the ancestral estrogen receptor.

scholarly journals Adrenal and sex steroid receptor evolution: environmental implications

2001 ◽  
Vol 26 (2) ◽  
pp. 119-125 ◽  
Author(s):  
ME Baker
Keyword(s):  
Nuclear Receptors ◽  
Protein Interactions ◽  
Steroid Receptors ◽  
Sequence Divergence ◽  
Heat Shock Protein 90 ◽  
Steroid Receptor ◽  
Retinoid X Receptor ◽  
Sex Steroid ◽  
Environmental Implications ◽  
Sex Steroid Receptors

The nuclear receptor family responds to a diverse group of ligands, including steroids, retinoids, thyroid hormone, prostaglandins and fatty acids. Previous sequence analyses of adrenal and sex steroid receptors indicate that they form a clade separate from other nuclear receptors. However, the relationships of adrenal and sex steroid receptors to each other and to their ancestors are not fully understood. We have used new information from androgen, estrogen, mineralocorticoid and progesterone receptors in fish to better resolve the phylogeny of adrenal and sex steroid receptors. Sequence divergence between fish and mammalian steroid receptors correlates with differences in steroid specificity, suggesting that phylogeny needs to be considered in evaluating the endocrine effects of xenobiotics. Among the vertebrate steroid receptors, the most ancient is the estrogen receptor. The phylogeny indicates that adrenal and sex steroid receptors arose in a jawless fish or a protochordate and that changes in the sequence of the hormone-binding domain have slowed considerably in land vertebrates. The retinoid X receptor clade is closest to the adrenal and sex steroid receptor clade. Retinoid X receptor is noteworthy for its ability to form dimers with other nuclear receptors, an important mechanism for regulating the action of retinoid X receptor and its dimerization partners. In contrast, the adrenal and sex steroid receptors bind to DNA as homodimers. Moreover, unliganded adrenal and sex steroid receptors form complexes with heat shock protein 90. Thus, the evolution of adrenal and sex steroid receptors involved changes in protein-protein interactions as well as ligand recognition.

scholarly journals Reduction of Coactivator Expression by Antisense Oligodeoxynucleotides Inhibits ERα Transcriptional Activity and MCF-7 Proliferation

2002 ◽  
Vol 16 (2) ◽  
pp. 253-270 ◽  
Author(s):  
Ilaria T. R. Cavarretta ◽  
Ratna Mukopadhyay ◽  
David M. Lonard ◽  
Lex M. Cowsert ◽  
C. Frank Bennett ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Activity ◽  
Steroid Receptors ◽  
Biological Effects ◽  
Steroid Receptor ◽  
Transient Transfection ◽  
Antisense Oligodeoxynucleotides ◽  
Steroid Receptor Coactivator ◽  
Mcf 7

Abstract Steroid receptor RNA activator (SRA) is a novel coactivator for steroid receptors that acts as an RNA molecule, whereas steroid receptor coactivator (SRC) family members, such as steroid receptor coactivator-1 (SRC-1) and transcriptional intermediary factor 2 (TIF2) exert their biological effects as proteins. Individual overexpression of each of these coactivators, which can form multimeric complexes in vivo, results in stimulated ERα transcriptional activity in transient transfection assays. However there is no information on the consequences of reducing SRC-1, TIF2, or SRA expression, singly or in combination, on ERα transcriptional activity. We therefore developed antisense oligodeoxynucleotides (asODNs) to SRA, SRC-1, and TIF2 mRNAs, which rapidly and specifically reduced the expression of each of these coactivators. ERα-dependent gene expression was reduced in a dose-dependent fashion by up to 80% in cells transfected with these oligonucleotides. Furthermore, treatment of cells with combinations of SRA, SRC-1, and TIF2 asODNs reduced ERα transcriptional activity to an extent greater than individual asODN treatment alone, suggesting that these coactivators cooperate, in at least an additive fashion, to activate ERα-dependent target gene expression. Finally, treatment of MCF-7 cells with asODN against SRC-1 and TIF2 revealed a requirement of these coactivators, but not SRA, for hormone-dependent DNA synthesis and induction of estrogen-dependent pS2 gene expression, indicating that SRA and SRC family coactivators can fulfill specific functional roles. Taken together, we have developed a rapid method to reduce endogenous coactivator expression that enables an assessment of the in vivo role of specific coactivators on ERα biological action and avoids potential artifacts arising from overexpression of coactivators in transient transfection assays.

Sign in / Sign up

Export Citation Format

Share Document