scholarly journals A Long-Acting, Mono-PEGylated Human Growth Hormone Analog Is a Potent Stimulator of Weight Gain and Bone Growth in Hypophysectomized Rats

Endocrinology ◽  
2007 ◽  
Vol 148 (4) ◽  
pp. 1590-1597 ◽  
Author(s):  
George N. Cox ◽  
Mary S. Rosendahl ◽  
Elizabeth A. Chlipala ◽  
Darin J. Smith ◽  
Sharon J. Carlson ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Weight Gain ◽  
Human Growth Hormone ◽  
Bone Growth ◽  
Human Growth ◽  
Potent Stimulator ◽  
Hormone Analog ◽  
Hypophysectomized Rats ◽  
Long Acting

Development of a long acting human growth hormone analog suitable for once a week dosing

2013 ◽  
Vol 23 (2) ◽  
pp. 402-406 ◽  
Author(s):  
Moorthy S.S. Palanki ◽  
Abhijit Bhat ◽  
Ben Bolanos ◽  
Florence Brunel ◽  
Joselyn Del Rosario ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Hormone Analog ◽  
Long Acting

A new bioassay for human growth hormone based on incorporation of labelled proline into skin

1982 ◽  
Vol 95 (1) ◽  
pp. 81-86 ◽  
Author(s):  
C. G. Rudman ◽  
Rose E. Gaines Das ◽  
J. A. Parsons
Keyword(s):  
Growth Hormone ◽  
Weight Gain ◽  
Human Growth Hormone ◽  
Dose Response ◽  
Human Growth ◽  
Hypophysectomized Rats ◽  
In Vivo Bioassay ◽  
New Bioassay ◽  
Average Index

An in-vivo bioassay for human growth hormone (hGH) has been developed, based on the dose-related enhancement of radioactivity in skin of hypophysectomized rats given daily s.c. injections of hGH and a single i.p. injection of labelled proline. The measured radioactivity, taken as the response, was shown to be independent of the site at which the skin was sampled. Valid dose–response relationships were obtained after as little as 3 days of treatment with GH. The assays by this method are shown to be more precise than the widely used weight-gain and tibial-width bioassays. Assays carried out gave an average index of precision of 0·036.

Reduced and S-carboxymethylated human growth hormone: a probe for diabetogenic action

1984 ◽  
Vol 247 (5) ◽  
pp. E639-E644
Author(s):  
C. M. Cameron ◽  
J. L. Kostyo ◽  
J. A. Rillema ◽  
S. E. Gennick
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Mouse Mammary Gland ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Hypophysectomized Rats

The biological activity profile of reduced and S-carboxymethylated human growth hormone (RCM-hGH) was determined to establish its suitability for study of the diabetogenic property of hGH. RCM-hGH was found to have greatly attenuated in vivo growth-promoting activity in the 9-day weight-gain test in hypophysectomized rats (approximately 1%) and to have a similar low order of in vitro activity in stimulating amino acid incorporation into the protein of the isolated rat diaphragm. RCM-hGH also only had approximately 1% of the in vitro insulin-like activity of the native hormone on isolated adipose tissue from hypophysectomized rats. In contrast, RCM-hGH retained substantial in vivo diabetogenic activity in the ob/ob mouse, appearing to have approximately 50% of the activity of the native hormone. RCM-hGH was also found to retain significant, although attenuated (25%), in vitro lactogenic activity when tested for the ability to stimulate amino acid incorporation into a casein-rich protein fraction in mouse mammary gland explants. Because RCM-hGH exhibits a high degree of diabetogenic activity, although lacking significant anabolic or insulin-like activities, it will be useful as a "monovalent" probe for the study of the molecular mechanism of the diabetogenic action of GH.

scholarly journals Human Growth Hormone Induces SOCS3 and CIS mRNA Increase in the Hypothalamic Neurons of Hypophysectomized Rats

2004 ◽  
Vol 51 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Yoko KASAGI ◽  
Reiko TOKITA ◽  
Tomoko NAKATA ◽  
Toshihiro IMAKI ◽  
Shiro MINAMI
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Hypothalamic Neurons ◽  
Hypophysectomized Rats

PREPARATION OF HUMAN GROWTH HORMONE

1961 ◽  
Vol 23 (3) ◽  
pp. 285-NP ◽  
Author(s):  
A. L. C. WALLACE ◽  
K. A. FERGUSON
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
High Yield ◽  
Pituitary Hormone ◽  
Simple Method ◽  
Anterior Pituitary Hormone ◽  
Epiphysial Cartilage ◽  
Diethylaminoethyl Cellulose ◽  
Hypophysectomized Rats

SUMMARY A simple method for the preparation of human growth hormone using chromatography on diethylaminoethyl-cellulose is described. Material prepared in this way, when assayed by growth of the tibial epiphysial cartilage in hypophysectomized rats, is at least as active as material prepared by published methods and is obtained in high yield. The only other anterior pituitary hormone activity present in any concentration is prolactin.

DOSE REGIMENS OF HUMAN GROWTH HORMONE: EFFECTS OF CONTINUOUS INFUSION AND OF A GELATIN VEHICLE ON GROWTH IN RATS AND RATE OF ABSORPTION IN RABBITS

1980 ◽  
Vol 87 (2) ◽  
pp. 303-312 ◽  
Author(s):  
P. MARY COTES ◽  
W. A. BARTLETT ◽  
ROSE E. GAINES DAS ◽  
P. FLECKNELL ◽  
R. TERMEER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Plasma Levels ◽  
Time Course ◽  
Human Growth ◽  
Daily Injection ◽  
Glycine Buffer ◽  
Hormone Effects ◽  
Rate Of Absorption ◽  
Hypophysectomized Rats

Different methods for administration of human growth hormone (hGH) have been examined with a view to efficient use of the limited amounts of hGH at present available for clinical use. We found that in hypophysectomized rats (1) hGH administered by continuous subcutaneous infusion induced a greater increase in body weight (referred to throughout as growth) than hGH administered by intermittent (daily) injection and (2) intermittent injections of hGH dissolved in 16% gelatin induced more growth than hGH dissolved in a glycine buffer. It was further found that (1) hGH dissolved in 16% gelatin compared with hGH dissolved in a glycine buffer induced lower maximal levels of immunoreactive plasma hGH and between 7 and 9 h after treatment higher plasma levels when injected subcutaneously in rabbits, (2) 125I-labelled hGH added as a tracer to hGH in gelatin was removed more slowly from subcutaneous injection sites in rabbits than 125I-labelled hGH given with hGH in glycine buffer and (3) changes in the ratio of hGH to gelatin had little effect on the time-course of plasma levels of hGH in the rabbit. Addition of the protease inhibitors aprotinin or 6-aminohexanoic acid, to injection of hGH in gelatin or glycine did not induce any consistent increase in plasma levels of hGH.

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