scholarly journals Transcriptional Profiling of Estrogen-Regulated Gene Expression via Estrogen Receptor (ER) α or ERβ in Human Osteosarcoma Cells: Distinct and Common Target Genes for These Receptors

Endocrinology ◽  
2004 ◽  
Vol 145 (7) ◽  
pp. 3473-3486 ◽  
Author(s):  
Fabio Stossi ◽  
Daniel H. Barnett ◽  
Jonna Frasor ◽  
Barry Komm ◽  
C. Richard Lyttle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Target Genes ◽  
Transcriptional Profiling ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Regulated Gene Expression ◽  
Common Target

scholarly journals Functional Interference Between AP-1 and the Vitamin D Receptor on Osteocalcin Gene Expression in Human Osteosarcoma Cells

1994 ◽  
Vol 224 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Tiina Jaaskelainen ◽  
Asta Pirskanen ◽  
Sanna Ryhanen ◽  
Jorma J. Palvimo ◽  
Hector F. Deluca ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Functional Interference ◽  
Human Osteosarcoma Cells ◽  
Osteocalcin Gene

Recombinant Baculoviruses Used to Study Estrogen Receptor Function in Human Osteosarcoma Cells

2003 ◽  
Vol 1 (6) ◽  
pp. 801-810 ◽  
Author(s):  
William C. Clay ◽  
J. Patrick Condreay ◽  
Linda B. Moore ◽  
Susan L. Weaver ◽  
Michael A. Watson ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Recombinant Baculoviruses ◽  
Receptor Function ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Stable Inhibition of Specific Estrogen Receptor α (ERα) Phosphorylation Confers Increased Growth, Migration/Invasion, and Disruption of Estradiol Signaling in MCF-7 Breast Cancer Cells

Endocrinology ◽  
2012 ◽  
Vol 153 (9) ◽  
pp. 4144-4159 ◽  
Author(s):  
B. P. Huderson ◽  
T. T. Duplessis ◽  
C. C. Williams ◽  
H. C. Seger ◽  
C. G. Marsden ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Estrogen Receptor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Target Genes ◽  
Expression Patterns ◽  
Estrogen Receptor Α ◽  
Regulated Gene Expression ◽  
Mcf 7

Elevated phosphorylation of estrogen receptor α (ERα) at serines 118 (S118) and 167 (S167) is associated with favorable outcome for tamoxifen adjuvant therapy and may serve as surrogate markers for a functional ERα signaling pathway in breast cancer. It is possible that loss of phosphorylation at S118 and/or S167 could disrupt ERα signaling, resulting in aggressive ERα-independent breast cancer cells. To this end, MCF-7 breast cancer cells were stably transfected with an ERα-specific short hairpin RNA that reduced endogenous ERα. The resulting cell line was stably transfected with wild-type ERα (ER-AB cells), or ERα containing serine to alanine mutation at S118 or S167 (S118A cells and S167A cells, respectively). These stable cell lines expressed approximately equivalent ERα compared with parental MCF-7 cells and were evaluated for growth, morphology, migration/invasion, and ERα-regulated gene expression. S118A cells and S167A cells exhibited increased growth and migration/invasion in vitro. Forward- and side-scatter flow cytometry revealed that S167A cells were smaller in size, and both S118A and S167A cells exhibited less cellular complexity. S118A and S167A cells expressed pancytokeratin and membrane localization of β-catenin and did not express vimentin, indicating retention of epithelial lineage markers. Expression of ERα-target genes and other genes regulated by ERα signaling or involved in breast cancer were markedly altered in both S118A and S167A cells. In summary, attenuated phosphorylation of ERα at S118 and S167 significantly affected cellular physiology and behavior in MCF-7 breast cancer cells, resulting in increased growth, migration/invasion, compromised expression of ERα target genes, and markedly altered gene expression patterns.

Interactions between estrogen and mechanical strain effects on U2OS human osteosarcoma cells are not influenced by estrogen receptor type

Bone ◽  
2004 ◽  
Vol 35 (5) ◽  
pp. 1127-1135 ◽  
Author(s):  
Florence Lima ◽  
Laurence Vico ◽  
Marie-Hélène Lafage-Proust ◽  
Paul van der Saag ◽  
Christian Alexandre ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Mechanical Strain ◽  
Receptor Type ◽  
Osteosarcoma Cells ◽  
Strain Effects ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells
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