scholarly journals Immortalization of Epididymal Epithelium in Transgenic Mice Expressing Simian Virus 40 T Antigen: Characterization of Cell Lines and Regulation of the Polyoma Enhancer Activator 3

Endocrinology ◽  
2004 ◽  
Vol 145 (1) ◽  
pp. 437-446 ◽  
Author(s):  
Petra Sipilä ◽  
Ramin Shariatmadari ◽  
Ilpo T. Huhtaniemi ◽  
Matti Poutanen
Keyword(s):  
Transgenic Mice ◽  
Cell Lines ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen

Establishment of Gastric Surface Mucous Cell Lines from Transgenic Mice Harboring Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene

1993 ◽  
Vol 209 (2) ◽  
pp. 382-387 ◽  
Author(s):  
Norifumi Sugiyama ◽  
Yoshiaki Tabuchi ◽  
Tadashi Horiuchi ◽  
Masuo Obinata ◽  
Mitsuru Furusawa
Keyword(s):  
Transgenic Mice ◽  
Cell Lines ◽  
Mucous Cell ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Antigen Gene ◽  
Surface Mucous Cell

scholarly journals Analysis of a transgenic mouse containing simian virus 40 and v-myc sequences.

1985 ◽  
Vol 5 (4) ◽  
pp. 642-648 ◽  
Author(s):  
J A Small ◽  
D G Blair ◽  
S D Showalter ◽  
G A Scangos
Keyword(s):  
Cell Lines ◽  
Choroid Plexus Papilloma ◽  
Simian Virus 40 ◽  
Soft Agar ◽  
Simian Virus ◽  
T Antigen ◽  
Primary Cell ◽  
Tissue Samples ◽  
Primary Cell Lines

Two plasmids, one containing the simian virus 40 (SV40) genome and the mouse metallothionein I gene and one containing the v-myc gene of avian myelocytomatosis virus MC29, were coinjected into mouse embryos. Of the 13 surviving mice, one, designated M13, contained both myc and SV40 sequences. This mouse developed a cranial bulge identified as a choroid plexus papilloma at 13 weeks and was subsequently sacrificed; tissue samples were taken for further analysis. Primary cell lines derived from these tissues contained both myc and SV40 DNA. No v-myc mRNA could be detected, although SV40 mRNA was present in all of the cell lines tested. T antigen also was expressed in all of the cell lines analyzed. These data suggest that SV40 expression was involved in the abnormalities of mouse M13 and was responsible for the transformed phenotype of the primary cell lines. Primary cell lines from this mouse were atypical in that the population rapidly became progressively more transformed with time in culture based on the following criteria: morphology, growth rate, and the ability to grow in soft agar and in serum-free medium. The data also suggest that factors present in the mouse regulated the ability of SV40 to oncogenically transform most cells and that in vitro culture of cells allowed them to escape those factors.

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