scholarly journals Yellow Fluorescent Protein-Tagged and Cyan Fluorescent Protein-Tagged Imaging Analysis of Glucocorticoid Receptor and Importins in Single Living Cells

Endocrinology ◽  
2003 ◽  
Vol 144 (9) ◽  
pp. 4070-4079 ◽  
Author(s):  
Masayuki Tanaka ◽  
Mayumi Nishi ◽  
Masafumi Morimoto ◽  
Tohru Sugimoto ◽  
Mitsuhiro Kawata
Keyword(s):  
Glucocorticoid Receptor ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Living Cells ◽  
Cyan Fluorescent Protein ◽  
Imaging Analysis ◽  
Single Living Cells ◽  
Single Living

Imaging analysis of mineralocorticoid receptor and importins in single living cells by using GFP color variants

2005 ◽  
Vol 320 (3) ◽  
pp. 447-453 ◽  
Author(s):  
Masayuki Tanaka ◽  
Mayumi Nishi ◽  
Masafumi Morimoto ◽  
Tohru Sugimoto ◽  
Mitsuhiro Kawata
Keyword(s):  
Mineralocorticoid Receptor ◽  
Living Cells ◽  
Imaging Analysis ◽  
Single Living Cells ◽  
Single Living ◽  
Color Variants

Dynamics of insulin-stimulated translocation of GLUT4 in single living cells visualised using green fluorescent protein

FEBS Letters ◽  
1996 ◽  
Vol 393 (2-3) ◽  
pp. 179-184 ◽  
Author(s):  
Stephen P. Dobson ◽  
Callum Livingstone ◽  
Gwyn W. Gould ◽  
Jeremy M. Tavaré
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Living Cells ◽  
Green Fluorescent ◽  
Single Living Cells ◽  
Single Living

Real-time analysis of GLUT4 trafficking in single living cells using green-fluorescent protein

1997 ◽  
Vol 25 (3) ◽  
pp. 460S-460S
Author(s):  
J.M. Tavaré ◽  
S.P. Dobson ◽  
C. Livingstone ◽  
A.A. Culbert ◽  
P.B. Oatey ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Real Time ◽  
Fluorescent Protein ◽  
Living Cells ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Green Fluorescent ◽  
Single Living Cells ◽  
Single Living

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Sign in / Sign up

Export Citation Format

Share Document