scholarly journals Hormone-Sensitive Lipase Deficiency in Mice Causes Lipid Storage in the Adrenal Cortex and Impaired Corticosterone Response to Corticotropin Stimulation

Endocrinology ◽  
2002 ◽  
Vol 143 (9) ◽  
pp. 3333-3340 ◽  
Author(s):  
Hong Li ◽  
Michèle Brochu ◽  
Shu Pei Wang ◽  
Leila Rochdi ◽  
Mylène Côté ◽  
...  
Keyword(s):  
Adrenal Cortex ◽  
Lipid Storage ◽  
Hormone Sensitive Lipase ◽  
Corticosterone Response ◽  
Hormone Sensitive ◽  
Corticotropin Stimulation

Hormone-sensitive lipase activity and triacylglycerol hydrolysis are decreased in rat soleus muscle by cyclopiazonic acid

2003 ◽  
Vol 285 (2) ◽  
pp. E412-E419 ◽  
Author(s):  
Matthew J. Watt ◽  
Gregory R. Steinberg ◽  
G. J. F. Heigenhauser ◽  
Lawrence L. Spriet ◽  
David J. Dyck
Keyword(s):  
Soleus Muscle ◽  
Kinase Inhibitor ◽  
Energy State ◽  
Muscle Tension ◽  
Cyclopiazonic Acid ◽  
Lipid Storage ◽  
Hormone Sensitive Lipase ◽  
Cellular Energy ◽  
Triacylglycerol Hydrolysis ◽  
Hormone Sensitive

Cyclopiazonic acid (CPA) is a sarcoplasmic reticulum Ca2+-ATPase inhibitor that increases intracellular calcium. The role of CPA in regulating the oxidation and esterification of palmitate, the hydrolysis of intramuscular lipids, and the activation of hormone-sensitive lipase (HSL) was examined in isolated rat soleus muscles at rest. CPA (40 μM) was added to the incubation medium to levels that resulted in subcontraction increases in muscle tension, and lipid metabolism was monitored using the previously described pulse-chase procedure. CPA did not alter the cellular energy state, as reflected by similar muscle contents of ATP, phosphocreatine, free AMP, and free ADP. CPA increased total palmitate uptake into soleus muscle (11%, P < 0.05) and was without effect on palmitate oxidation. This resulted in greater esterification of exogenous palmitate into the triacylglycerol (18%, P < 0.05) and phospholipid (89%, P < 0.05) pools. CPA decreased ( P < 0.05) intramuscular lipid hydrolysis, and this occurred as a result of reduced HSL activity (20%, P < 0.05). Incubation of muscles with 3 mM caffeine, which is also known to increase Ca2+ without affecting the cellular energy state, reduced HSL activity (24%, P < 0.05). KN-93, a calcium/calmodulin-dependent kinase inhibitor (CaMKII), blocked the effects of CPA and caffeine, and HSL activity returned to preincubation values. The results of the present study demonstrate that CPA simultaneously decreases intramuscular triacylglycerol (IMTG) hydrolysis and promotes lipid storage in isolated, intact soleus muscle. The decreased IMTG hydrolysis is likely mediated by reduced HSL activity, possibly via the CaMKII pathway. These responses are not consistent with the increased hydrolysis and decreased esterification observed in contracting muscle when substrate availability and the hormonal milieu are tightly controlled. It is possible that more powerful signals or a higher [Ca2+] may override the lipid-storage effect of the CPA-mediated effects during muscular contractions.

scholarly journals Ovarian Cycle-Specific Regulation of Adipose Tissue Lipid Storage by Testosterone in Female Nonhuman Primates

Endocrinology ◽  
2013 ◽  
Vol 154 (11) ◽  
pp. 4126-4135 ◽  
Author(s):  
Oleg Varlamov ◽  
Michael P. Chu ◽  
Whitney K. McGee ◽  
Judy L. Cameron ◽  
Robert W. O'Rourke ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Sex Hormones ◽  
Luteal Phase ◽  
Fatty Acid Uptake ◽  
Ovarian Cycle ◽  
Lipid Storage ◽  
Hormone Sensitive Lipase ◽  
Acid Uptake ◽  
Hormone Sensitive

Previous studies in rodents and humans suggest that hyperandrogenemia causes white adipose tissue (WAT) dysfunction in females, although the underlying mechanisms are poorly understood. In light of the differences in the length of the ovarian cycle between humans and rodents, we used a nonhuman primate model to elucidate the effects of chronic hyperandrogenemia on WAT function in vivo. Female rhesus macaques implanted with testosterone capsules developed insulin resistance and altered leptin secretion on a high-fat, Western-style diet. In control visceral WAT, lipolysis and hormone-sensitive lipase expression were upregulated during the luteal phase compared with the early follicular (menses) phase of the ovarian cycle. Hyperandrogenemia attenuated elevated lipolysis and hormone-sensitive lipase activity in visceral WAT during the luteal phase but not during menses. Under control conditions, insulin-stimulated Akt and Erk activation and fatty acid uptake in WAT were not significantly affected by the ovarian cycle. In contrast, testosterone treatment preferentially increased fatty acid uptake and insulin signaling at menses. The fatty acid synthase and glucose transporter-4 genes were upregulated by testosterone during the luteal phase. In summary, this study reveals ovarian stage-specific fluctuations in adipocyte lipolysis and suggests that male sex hormones increase and female sex hormones decrease lipid storage in female WAT.

Essential role of hormone-sensitive lipase (HSL) in the maintenance of lipid storage in Mycobacterium leprae-infected macrophages

2012 ◽  
Vol 52 (5) ◽  
pp. 285-291 ◽  
Author(s):  
Kazunari Tanigawa ◽  
Yang Degang ◽  
Akira Kawashima ◽  
Takeshi Akama ◽  
Aya Yoshihara ◽  
...  
Keyword(s):  
Essential Role ◽  
Mycobacterium Leprae ◽  
Lipid Storage ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive

scholarly journals Mechanism of hormone-stimulated lipolysis in adipocytes: translocation of hormone-sensitive lipase to the lipid storage droplet.

1992 ◽  
Vol 89 (18) ◽  
pp. 8537-8541 ◽  
Author(s):  
J. J. Egan ◽  
A. S. Greenberg ◽  
M. K. Chang ◽  
S. A. Wek ◽  
M. C. Moos ◽  
...  
Keyword(s):  
Lipid Storage ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive

scholarly journals Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.

1981 ◽  
Vol 256 (12) ◽  
pp. 6311-6320
Author(s):  
G. Fredrikson ◽  
P. Strålfors ◽  
N.O. Nilsson ◽  
P. Belfrage
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive ◽  
Rat Adipose Tissue
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