Mullerian inhibiting substance messenger ribonucleic acid expression in granulosa and Sertoli cells coincides with their mitotic activity

Endocrinology ◽  
1992 ◽  
Vol 131 (2) ◽  
pp. 854-862 ◽  
Author(s):  
S. Hirobe
Keyword(s):  
Mitotic Activity ◽  
Sertoli Cells ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid ◽  
Müllerian Inhibiting Substance ◽  
Mullerian Inhibiting Substance

scholarly journals Immunocytochemical localization of Mullerian inhibiting substance in the rough endoplasmic reticulum and Golgi apparatus in Sertoli cells of the neonatal calf testis using a monoclonal antibody.

1984 ◽  
Vol 32 (6) ◽  
pp. 649-654 ◽  
Author(s):  
M Hayashi ◽  
H Shima ◽  
K Hayashi ◽  
R L Trelstad ◽  
P K Donahoe
Keyword(s):  
Monoclonal Antibody ◽  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Rough Endoplasmic Reticulum ◽  
Sertoli Cells ◽  
Neonatal Calf ◽  
Müllerian Inhibiting Substance ◽  
Pap Method ◽  
Unlabeled Antibody ◽  
Mullerian Inhibiting Substance

Mullerian Inhibiting Substance (MIS) has been localized in the Sertoli cells of the neonatal calf testis using preembedding immunoperoxidase techniques and a monoclonal antibody which almost completely blocks the biological activity of MIS. Both the peroxidase-labeled antibody method using a peroxidase-conjugated F(ab')2 fragment of IgG as a second antibody and the unlabeled antibody peroxidase-antiperoxidase (PAP) method using Fab fragments of the PAP complex were employed. With both methods, MIS was demonstrated within the cisternae of the rough endoplasmic reticulum (RER) and the Golgi apparatus. In the Golgi, MIS was concentrated in the transmost cisternae especially at their peripheral expansions. This study indicates that MIS is synthesized in the RER and transported to the Golgi apparatus, presumably for glycosidation, before secretion from Golgi derived vacuoles.

scholarly journals Rapid and Profound Suppression of Messenger Ribonucleic Acid Encoding Follicle-Stimulating Hormoneβ by Inhibin from Primate Sertoli Cells

1989 ◽  
Vol 3 (2) ◽  
pp. 280-287 ◽  
Author(s):  
Barbara Attardi ◽  
Hugh S. Keeping ◽  
Stephen J. Winters ◽  
Fumikazu Kotsuji ◽  
Richard A. Maurer ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid
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