scholarly journals Liposome-based formulations for the antibiotic nonapeptide Leucinostatin A: Fourier transform infrared spectroscopy characterization and in vivo toxicologic study

2000 ◽  
Vol 1 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Maurizio Ricci ◽  
Paola Sassi ◽  
Claudio Nastruzzi ◽  
Carlo Róssi
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared

scholarly journals Cell apoptosis specific marker found by Fourier Transform Infrared Spectroscopy

2004 ◽  
Vol 18 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Silvia Gaudenzi ◽  
Deleana Pozzi ◽  
Paolo Toro ◽  
Ida Silvestri ◽  
Stefania Morrone ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Ph Value ◽  
Fourier Transform Infrared ◽  
Specific Marker ◽  
Chemical Agent

We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm−1and 2800 cm−1, a “marker band” of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non‒destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologiesin vivo.

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