scholarly journals Endocannabinoid metabolomics: A novel liquid chromatography-mass spectrometry reagent for fatty acid analysis

2006 ◽  
Vol 8 (4) ◽  
pp. E655-E660 ◽  
Author(s):  
John Williams ◽  
Lakshmipathi Pandarinathan ◽  
Jodi Anne Wood ◽  
Paul Vouros ◽  
Alexandros Makriyannis
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

scholarly journals Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops

2019 ◽  
Author(s):  
Alina Chernova ◽  
Pavel Mazin ◽  
Svetlana Goryunova ◽  
Denis Goryunov ◽  
Yakov Demurin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Ultra Performance Liquid Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Chromatography Mass Spectrometry ◽  
Oilseed Crops ◽  
Oil Crops ◽  
Chromatography Mass Spectrometry

Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs in rapeseed, UPLC-MS appeared to be more sensitive, detecting about 2.5 times higher number of FAs in both plants. In addition to even-chain FAs, UPLC-MS was able to detect odd-chain Fas. The longest FA detected using GC-FID was a FA with 24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with the tails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MS has a great potential to be used for the assessment of the FA profile of oil crops.

scholarly journals Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry

2018 ◽  
Vol 56 (2) ◽  
pp. 190-197 ◽  
Author(s):  
Zhen Chen ◽  
Yue Wu ◽  
Rojeet Shrestha ◽  
Zijun Gao ◽  
Yaoyao Zhao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Chain Fatty Acids

Background Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. Methods Blood samples from human subjects ( n = 547, male/female = 246/301, age 58.85 ± 12.57) were collected. Saponification was applied to obtain total fatty acid. After derivatization by 2-nitrophenylhydrazine, fatty acid 4:0 and fatty acid 6:0 were measured by liquid chromatography-mass spectrometry. Results The developed method exhibited good linearity (R2 = 0.9996 for both). All the coefficients of variation of reproducibility and accuracy for fatty acid 4:0 and fatty acid 6:0 ranged 3.0%−6.1%, with the average recoveries of 87.8%−102.4% and 92.2%−98.2%, respectively. In all the samples, the concentration of fatty acid 4:0 (162.4 ± 76.4 μmol/L) was significantly higher than fatty acid 6:0 (2.0 ± 2.5 μmol/L, P < 0.001). Furthermore, the esterified form was predominant in both fatty acid 4:0 and fatty acid 6:0 (98.2% and 82.4% of total fatty acids, respectively). Besides, short-chain fatty acids showed no significant differences with regard to sex or age differences. Conclusion This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood.

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