Endolysosomal Patch Clamping

2020 ◽  
pp. 45-76
Author(s):  
Cheng-Chang Chen ◽  
Christian Grimm ◽  
Christian Wahl-Schott ◽  
Martin Biel
Keyword(s):  
Patch Clamping

Novel processing for a polymer patch clamping system

2009 ◽  
Author(s):  
S. Wilson ◽  
W. Pfleging ◽  
M. Bruns ◽  
A. Welle ◽  
P.B. Kirby
Keyword(s):  
Patch Clamping ◽  
Clamping System ◽  
Novel Processing

scholarly journals Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot5014-pdb.prot5014 ◽  
Author(s):  
W. Zhang ◽  
S. E. Nilson ◽  
S. M. Assmann
Keyword(s):  
Guard Cell ◽  
Patch Clamping ◽  
Whole Cell ◽  
Guard Cell Protoplasts

Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording

Lab on a Chip ◽  
2009 ◽  
Vol 9 (16) ◽  
pp. 2370 ◽  
Author(s):  
Chang-Yu Chen ◽  
Ting-Yuan Tu ◽  
Chang-Hung Chen ◽  
De-Shien Jong ◽  
Andrew M. Wo
Keyword(s):  
Ion Channel ◽  
High Yield ◽  
Patch Clamping

Voltage and patch clamping with microelectrodes

1985 ◽  
Vol 10 (12) ◽  
pp. 494
Author(s):  
Julian I. Schroeder
Keyword(s):  
Patch Clamping

scholarly journals Toxoplasma gondii injected neurons localize to the cortex and striatum and have altered firing

2021 ◽  
Author(s):  
Oscar A. Mendez ◽  
Emiliano Flores Machado ◽  
Jing Lu ◽  
Anita A. Koshy
Keyword(s):  
Toxoplasma Gondii ◽  
Single Neuron ◽  
Latent Infection ◽  
Ex Vivo ◽  
Medium Spiny Neurons ◽  
Patch Clamping ◽  
Spiny Neurons ◽  
The Brain ◽  
Mapping Program

AbstractToxoplasma gondii is an intracellular parasite that causes a long-term latent infection of neurons. Using a custom MATLAB-based mapping program in combination with a mouse model that allows us to permanently mark neurons injected with parasite proteins, we found that Toxoplasma-injected neurons (TINs) are heterogeneously distributed in the brain, primarily localizing to the cortex followed by the striatum. Using immunofluorescence co-localization assays, we determined that cortical TINs are commonly (>50%) excitatory neurons (FoxP2+) and that striatal TINs are often (>65%) medium spiny neurons (MSNs) (FoxP2+). As MSNs have highly characterized electrophysiology, we used ex vivo slices from infected mice to perform single neuron patch-clamping on striatal TINs and neighboring uninfected MSNs (bystander MSNs). These studies demonstrated that TINs have highly abnormal electrophysiology, while the electrophysiology of bystander MSNs was akin to that of MSNs from uninfected mice. Collectively, these data offer new neuroanatomic and electrophysiologic insights into CNS toxoplasmosis.

Patch Clamping

Cell Biology ◽  
2006 ◽  
pp. 395-403
Author(s):  
B RYCROFT ◽  
F HALLIDAY ◽  
A GIBB
Keyword(s):  
Patch Clamping
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