Chloride Transport across the Lysosomal Membrane

2020 ◽  
pp. 19-44
Author(s):  
Sonali Saha ◽  
Anja Blessing ◽  
Thomas J. Jentsch
Keyword(s):  
Chloride Transport ◽  
Lysosomal Membrane

scholarly journals Characteristics of a lysosomal membrane transport system for tyrosine and other neutral amino acids in rat thyroid cells.

1986 ◽  
Vol 261 (36) ◽  
pp. 17107-17112
Author(s):  
J Bernar ◽  
F Tietze ◽  
L D Kohn ◽  
I Bernardini ◽  
G S Harper ◽  
...  
Keyword(s):  
Amino Acids ◽  
Membrane Transport ◽  
Transport System ◽  
Lysosomal Membrane ◽  
Thyroid Cells ◽  
Neutral Amino Acids ◽  
Rat Thyroid

scholarly journals Trospium Chloride Transport by Mouse Drug Carriers of the Slc22 and Slc47 Families

2020 ◽  
Vol 22 (1) ◽  
pp. 22
Author(s):  
Matthias Gorecki ◽  
Simon F. Müller ◽  
Regina Leidolf ◽  
Joachim Geyer
Keyword(s):  
Knockout Mouse ◽  
Organic Cation ◽  
Chloride Transport ◽  
Drug Carriers ◽  
Hek293 Cells ◽  
Low Permeability ◽  
Organic Cation Transporters ◽  
Trospium Chloride ◽  
Individual Role ◽  
Positively Charged

Background: The muscarinic receptor antagonist trospium chloride (TCl) is used for pharmacotherapy of the overactive bladder syndrome. TCl is a hydrophilic positively charged drug. Therefore, it has low permeability through biomembranes and requires drug transporters for distribution and excretion. In humans, the organic cation transporters OCT1 and OCT2 and the multidrug and toxin extrusion MATE1 and MATE2-K carriers showed TCl transport. However, their individual role for distribution and excretion of TCl is unclear. Knockout mouse models lacking mOct1/mOct2 or mMate1 might help to clarify their role for the overall pharmacokinetics of TCl. Method: In preparation of such experiments, TCl transport was analyzed in HEK293 cells stably transfected with the mouse carriers mOct1, mOct2, mMate1, and mMate2, respectively. Results: Mouse mOct1, mOct2, and mMate1 showed significant TCl transport with Km values of 58.7, 78.5, and 29.3 µM, respectively. In contrast, mMate2 did not transport TCl but showed MPP+ transport with Km of 60.0 µM that was inhibited by the drugs topotecan, acyclovir, and levofloxacin. Conclusion: TCl transport behavior as well as expression pattern were quite similar for the mouse carriers mOct1, mOct2, and mMate1 compared to their human counterparts.

A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy

2020 ◽  
Author(s):  
Karla Alvarez-Valadez ◽  
Allan Sauvat ◽  
Hélène Fohrer-Ting ◽  
Christophe Klein ◽  
Oliver Kepp ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Permeabilization

Lysosomal membrane proteins exposed to melanin in melanocyte B16F10

2016 ◽  
Vol 8 (3) ◽  
pp. 253-257
Author(s):  
Seung Hyuck Bang ◽  
Dong Jun Park ◽  
Yang-Hoon Kim ◽  
Jiho Min
Keyword(s):  
Membrane Proteins ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Proteins
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