Reminiscences of My Early Work: II. Transverse Spread of Contraction Initiated by Local Membrane Depolarization in Single Muscle Fibers

2017 ◽  
pp. 203-239
Author(s):  
Haruo Sugi
Keyword(s):  
Muscle Fibers ◽  
Membrane Depolarization ◽  
Single Muscle

scholarly journals DISSECTION AND ELECTROPHORETIC PROTEIN ANALYSIS OF SINGLE MUSCLE FIBERS FROM HISTOLOGICALLY IDENTIFIED FREEZE-DRIED SECTIONS: APPLICATION TO MUSCLE FIBERS WITH RIMMED VACUOLES

1983 ◽  
Vol 4 (5) ◽  
pp. 459-466 ◽  
Author(s):  
ITARU TOYOSHIMA ◽  
KEIKO TANAKA ◽  
NOBUYOSHI FUKUHARA ◽  
TOSHIHIDE KUMAMOTO ◽  
TADASHI MIYATAKE
Keyword(s):  
Muscle Fibers ◽  
Protein Analysis ◽  
Single Muscle ◽  
Freeze Dried

scholarly journals Atp-Induced Membrane Depolarization Relates to Skeletal Muscle Fibers Plasticity

2013 ◽  
Vol 104 (2) ◽  
pp. 290a
Author(s):  
Mariana Casas ◽  
Paola Llanos ◽  
Gonzalo Jorquera ◽  
Jorge Hidalgo ◽  
Sonja Buvinic ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Membrane Depolarization ◽  
Induced Membrane ◽  
Skeletal Muscle Fibers

Contraction-induced injury to single muscle fibers: velocity of stretch does not influence the force deficit

1998 ◽  
Vol 275 (6) ◽  
pp. C1548-C1554 ◽  
Author(s):  
Gordon S. Lynch ◽  
John A. Faulkner
Keyword(s):  
Null Hypothesis ◽  
Muscle Injury ◽  
Isometric Force ◽  
Muscle Fibers ◽  
Single Muscle ◽  
Control Value ◽  
Severity Of Injury ◽  
Before And After ◽  
The Difference ◽  
Permeabilized Fibers

We tested the null hypothesis that the severity of injury to single muscle fibers following a single pliometric (lengthening) contraction is not dependent on the velocity of stretch. Each single permeabilized fiber obtained from extensor digitorum longus muscles of rats was maximally activated and then exposed to a single stretch of either 5, 10, or 20% strain [% of fiber length ( L f)] at a velocity of 0.5, 1.0, or 2.0 L f /s. The force deficit, the difference between maximum tetanic isometric force (Po) before and after the stretch expressed as a percentage of the control value for Po before the stretch, provided an estimate of the magnitude of muscle injury. Despite a fourfold range from the lowest to the highest velocities, force deficits were not different among stretches of the same strain. At stretches of 20% strain, even an eightfold range of velocities produced no difference in the force deficit, although 40% of the fibers were torn apart at a velocity of 4 L f /s. We conclude that, within the range of velocities tolerated by single permeabilized fibers, the severity of contraction-induced injury is not related to the velocity of stretch.

MHC and sarcoplasmic reticulum protein isoforms in functionally overloaded cat plantaris muscle fibers

1996 ◽  
Vol 80 (4) ◽  
pp. 1296-1303 ◽  
Author(s):  
R. J. Talmadge ◽  
R. R. Roy ◽  
G. R. Chalmers ◽  
V. R. Edgerton
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Heavy Chain ◽  
Muscle Fibers ◽  
Protein Isoforms ◽  
Type I ◽  
Single Muscle ◽  
Plantaris Muscle ◽  
Plasmic Reticulum ◽  
Deep Region ◽  
Isoform Expression

To determine whether the adaptations in myosin heavy chain (MHC) isoform expression after functional overload (FO) are accompanied by commensurate adaptations in protein isoforms responsible for relaxation [sarco(endo)plasmic reticulum (SR) Ca(2+)-adenosinetriphosphatase (SERCA) and phospholamban (PHL)] in single muscle fibers, the isoforms of MHC and SERCA and the presence or absence of PHL were determined for cat plantaris fibers 3 mo after FO. In control plantaris the relative MHC isoform composition was 23% type I, 21% type IIa, and 56% type IIb. FO resulted in a shift toward slower isoforms (33% type I, 44% type IIa, and 23% type IIb). In the deep region of the plantaris the proportions of type I MHC and hybrid MHC fibers (containing type I and II MHCs) were 40 and 200% greater in FO cats, respectively. FO resulted in a 47% increase in the proportion of fibers containing only the slow SERCA isoform (SERCA2) and a 41% increase in the proportion of fibers containing PHL. The proportions of fibers containing type I MHC, SERCA2, and PHL in control and FO plantaris were linearly correlated. These data show that adaptations in MHC isoform expression are accompanied by commensurate adaptations in sarcoplasmic reticulum protein isoforms in single muscle fibers after FO.

scholarly journals Ultraslow contractile inactivation in frog skeletal muscle fibers.

1990 ◽  
Vol 96 (1) ◽  
pp. 47-56 ◽  
Author(s):  
C Caputo ◽  
P Bolaños
Keyword(s):  
Skeletal Muscle ◽  
Time Course ◽  
Muscle Fibers ◽  
Membrane Depolarization ◽  
Frog Muscle ◽  
Slow Time ◽  
Inactivation Curve ◽  
Lanthanum Ions ◽  
Skeletal Muscle Fibers ◽  
Spontaneous Relaxation

After a contracture response, skeletal muscle fibers enter into a state of contractile refractoriness or inactivation. Contractile inactivation starts soon after membrane depolarization, and causes spontaneous relaxation from the contracture response. Here we demonstrate that contractile inactivation continues to develop for tens of seconds if the membrane remains in a depolarized state. We have studied this phenomenon using short (1.5 mm) frog muscle fibers dissected from the Lumbricalis brevis muscles of the frog, with a two-microelectrode voltage-clamp technique. After a contracture caused by membrane depolarization to 0 mV, from a holding potential of -100 mV, a second contracture can be developed only if the membrane is repolarized beyond a determined potential value for a certain period of time. We have used a repriming protocol of 1 or 2 s at -100 mV. After this repriming period a fiber, if depolarized again to 0 mV, may develop a second contracture, whose magnitude and time course will depend on the duration of the period during which the fiber was maintained at 0 mV before the repriming process. With this procedure it is possible to demonstrate that the inactivation process builds up with a very slow time course, with a half time of approximately 35 s and completion in greater than 100 s. After prolonged depolarizations (greater than 100 s), the repriming time course is slower and the inactivation curve (obtained by plotting the extent of repriming against the repriming membrane potential) is shifted toward more negative potentials by greater than 30 mV when compared with similar curves obtained after shorter depolarizing periods (10-30 s). These results indicate that important changes occur in the physical state of the molecular moiety that is responsible for the inactivation phenomenon. The shift of the inactivation curve can be partially reversed by a low concentration (50 microM) of lanthanum ions. In the presence of 0.5 mM caffeine, larger responses can be obtained even after prolonged depolarization periods, indicating that the fibers maintain their capacity to liberate calcium.

The mutations m.5628T>C and m.8348A>G in single muscle fibers of a patient with chronic progressive external ophthalmoplegia

2012 ◽  
Vol 320 (1-2) ◽  
pp. 131-135 ◽  
Author(s):  
Juliana Gamba ◽  
Beatriz Hitomi Kiyomoto ◽  
Acary Souza Bulle de Oliveira ◽  
Alberto Alain Gabbai ◽  
Beny Schmidt ◽  
...  
Keyword(s):  
Muscle Fibers ◽  
Progressive External Ophthalmoplegia ◽  
Chronic Progressive External Ophthalmoplegia ◽  
Single Muscle

Size changes in single muscle fibers during fixation and embedding

1975 ◽  
Vol 7 (2) ◽  
pp. 383-387 ◽  
Author(s):  
Brenda R. Eisenberg ◽  
Bert A. Mobley
Keyword(s):  
Muscle Fibers ◽  
Single Muscle

scholarly journals Quantitative electrophoretic analysis of myosin heavy chains in single muscle fibers

2001 ◽  
Vol 90 (5) ◽  
pp. 1927-1935 ◽  
Author(s):  
Boris A. Tikunov ◽  
H. Lee Sweeney ◽  
Lawrence C. Rome
Keyword(s):  
Muscle Fibers ◽  
Electrophoretic Analysis ◽  
Single Fiber ◽  
Fiber Types ◽  
Single Muscle ◽  
Myosin Heavy Chains ◽  
Single Fibers ◽  
Heavy Chains ◽  
Cross Bridges ◽  
Whole Muscle

To better understand the molecular basis of the large variation in mechanical properties of different fiber types, there has been an intense effort to relate the mechanical and energetic properties measured in skinned single fibers to those of their constituent cross bridges. There is a significant technical obstacle, however, in estimating the number of cross bridges in a single fiber. In this study, we have developed a procedure for extraction and quantification of myosin heavy chains (MHCs) that permits the routine and direct measurement of the myosin content in single muscle fibers. To validate this method, we also compared MHC concentration measured in single fibers with the MHC concentration in whole fast-twitch (psoas and gracilis) and slow-twitch (soleus) muscles of rabbit. We found that the MHC concentration in intact psoas (184 μM) was larger than that in soleus (144 μM), as would be expected from their differing mitochondrial content and volume of myofibrils. We obtained excellent agreement between MHC concentration measured at the single fiber level with that measured at the whole muscle level. This not only verifies the efficacy of our procedure but also shows that the difference in concentration at the whole muscle level simply reflects the concentration differences in the constituent fiber types. This new procedure should be of considerable help in future attempts to determine kinetic differences in cross bridges from different fiber types.

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