Load-Dependent Mechanical Efficiency of Myosin Head Performance

2017 ◽  
pp. 155-170
Author(s):  
Haruo Sugi
Keyword(s):  
Myosin Head ◽  
Mechanical Efficiency

Orientation of actin filaments during motion in motility assay

1995 ◽  
Vol 53 ◽  
pp. 52-53
Author(s):  
J. Borejdo ◽  
S. Burlacu
Keyword(s):  
Actin Filaments ◽  
Thin Filament ◽  
Striated Muscle ◽  
Myosin Head ◽  
Classical Method ◽  
Polarization Of Fluorescence ◽  
Single Protein ◽  
Intracellular Organelles ◽  
Change Orientation ◽  
Active Entity

Polarization of fluorescence is a classical method to assess orientation or mobility of macromolecules. It has been a common practice to measure polarization of fluorescence through a microscope to characterize orientation or mobility of intracellular organelles, for example anisotropic bands in striated muscle. Recently, we have extended this technique to characterize single protein molecules. The scientific question concerned the current problem in muscle motility: whether myosin heads or actin filaments change orientation during contraction. The classical view is that the force-generating step in muscle is caused by change in orientation of myosin head (subfragment-1 or SI) relative to the axis of thin filament. The molecular impeller which causes this change resides at the interface between actin and SI, but it is not clear whether only the myosin head or both SI and actin change orientation during contraction. Most studies assume that observed orientational change in myosin head is a reflection of the fact that myosin is an active entity and actin serves merely as a passive "rail" on which myosin moves.

CRYO-Electron Microscopy of the Acto-Myosin-ATP Complex

1990 ◽  
Vol 48 (1) ◽  
pp. 248-249
Author(s):  
John Trinickt ◽  
Howard White
Keyword(s):  
Electron Microscopy ◽  
Atp Hydrolysis ◽  
Myosin Head ◽  
Bound State ◽  
Cross Linking ◽  
Weakly Bound ◽  
Cryo Electron Microscopy ◽  
Myosin Subfragment 1 ◽  
Attached State ◽  
High Fraction

The primary force of muscle contraction is thought to involve a change in the myosin head whilst attached to actin, the energy coming from ATP hydrolysis. This change in attached state could either be a conformational change in the head or an alteration in the binding angle made with actin. A considerable amount is known about one bound state, the so-called strongly attached state, which occurs in the presence of ADP or in the absence of nucleotide. In this state, which probably corresponds to the last attached state of the force-producing cycle, the angle between the long axis myosin head and the actin filament is roughly 45°. Details of other attached states before and during power production have been difficult to obtain because, even at very high protein concentration, the complex is almost completely dissociated by ATP. Electron micrographs of the complex in the presence of ATP have therefore been obtained only after chemically cross-linking myosin subfragment-1 (S1) to actin filaments to prevent dissociation. But it is unclear then whether the variability in attachment angle observed is due merely to the cross-link acting as a hinge.We have recently found low ionic-strength conditions under which, without resorting to cross-linking, a high fraction of S1 is bound to actin during steady state ATP hydrolysis. The structure of this complex is being studied by cryo-electron microscopy of hydrated specimens. Most advantages of frozen specimens over ambient temperature methods such as negative staining have already been documented. These include improved preservation and fixation rates and the ability to observe protein directly rather than a surrounding stain envelope. In the present experiments, hydrated specimens have the additional benefit that it is feasible to use protein concentrations roughly two orders of magnitude higher than in conventional specimens, thereby reducing dissociation of weakly bound complexes.

scholarly journals Analytical Modeling and Simulation of S-Drive Piezoelectric Actuators

Actuators ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 87
Author(s):  
Nicholas A. Jones ◽  
Jason Clark
Keyword(s):  
Element Model ◽  
Mechanical Efficiency ◽  
Compact Model ◽  
High Frequency Response ◽  
Macro Scale ◽  
Piezoelectric Structure ◽  
Modern Manufacturing ◽  
Manufacturing Techniques ◽  
Piezoelectric Structures ◽  
Improve Device

This paper presents a structural geometry for increasing piezoelectric deformation, which is suitable for both micro- and macro-scale applications. New and versatile microstructure geometries for actuators can improve device performance, and piezoelectric designs benefit from a high-frequency response, power density, and efficiency, making them a viable choice for a variety of applications. Previous works have presented piezoelectric structures capable of this amplification, but few are well-suited to planar manufacturing. In addition to this manufacturing difficulty, a large number of designs cannot be chained into longer elements, preventing them from operating at the macro-scale. By optimizing for both modern manufacturing techniques and composability, this structure excels as an option for a variety of macro- and micro-applications. This paper presents an analytical compact model of a novel dual-bimorph piezoelectric structure, and shows that this compact model is within 2% of a computer-distributed element model. Furthermore it compares the actuator’s theoretical performance to that of a modern actuator, showing that this actuator trades mechanical efficiency for compactness and weight savings.

scholarly journals Noninvasive Assessment of Neuromechanical Coupling and Mechanical Efficiency of Parasternal Intercostal Muscle during Inspiratory Threshold Loading

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1781
Author(s):  
Manuel Lozano-García ◽  
Luis Estrada-Petrocelli ◽  
Abel Torres ◽  
Gerrard F. Rafferty ◽  
John Moxham ◽  
...  
Keyword(s):  
Mechanical Efficiency ◽  
Intercostal Muscle ◽  
Wireless Devices ◽  
Clinical Value ◽  
Intercostal Muscles ◽  
Noninvasive Measurements ◽  
Standard Assessment ◽  
Mouth Pressure ◽  
Invasive Techniques ◽  
Increasing Load

This study aims to investigate noninvasive indices of neuromechanical coupling (NMC) and mechanical efficiency (MEff) of parasternal intercostal muscles. Gold standard assessment of diaphragm NMC requires using invasive techniques, limiting the utility of this procedure. Noninvasive NMC indices of parasternal intercostal muscles can be calculated using surface mechanomyography (sMMGpara) and electromyography (sEMGpara). However, the use of sMMGpara as an inspiratory muscle mechanical output measure, and the relationships between sMMGpara, sEMGpara, and simultaneous invasive and noninvasive pressure measurements have not previously been evaluated. sEMGpara, sMMGpara, and both invasive and noninvasive measurements of pressures were recorded in twelve healthy subjects during an inspiratory loading protocol. The ratios of sMMGpara to sEMGpara, which provided muscle-specific noninvasive NMC indices of parasternal intercostal muscles, showed nonsignificant changes with increasing load, since the relationships between sMMGpara and sEMGpara were linear (R2 = 0.85 (0.75–0.9)). The ratios of mouth pressure (Pmo) to sEMGpara and sMMGpara were also proposed as noninvasive indices of parasternal intercostal muscle NMC and MEff, respectively. These indices, similar to the analogous indices calculated using invasive transdiaphragmatic and esophageal pressures, showed nonsignificant changes during threshold loading, since the relationships between Pmo and both sEMGpara (R2 = 0.84 (0.77–0.93)) and sMMGpara (R2 = 0.89 (0.85–0.91)) were linear. The proposed noninvasive NMC and MEff indices of parasternal intercostal muscles may be of potential clinical value, particularly for the regular assessment of patients with disordered respiratory mechanics using noninvasive wearable and wireless devices.

Numerical simulation and experimental performance research of cylindrical vane pump

2021 ◽  
pp. 095440622110200
Author(s):  
Yiqi Cheng ◽  
Xinhua Wang ◽  
Waheed Ur Rehman ◽  
Tao Sun ◽  
Hasan Shahzad ◽  
...  
Keyword(s):  
Numerical Analysis ◽  
Rotational Velocity ◽  
Geometric Theory ◽  
Mechanical Efficiency ◽  
Operating Conditions ◽  
Experimental Results ◽  
Field Analysis ◽  
Total Efficiency ◽  
Three Dimensional Model ◽  
Vane Pump

This study presents a novel cylindrical vane pump based on the traditional working principle. The efficiency of the cylindrical vane pump was verified by experimental validation and numerical analysis. Numerical analysis, such as kinematics analysis, was performed in Pro/Mechanism and unsteady flow-field analysis was performed using ANSYS FLUENT. The stator surface equations were derived using the geometric theory of the applied spatial triangulation function. A three-dimensional model of the cylindrical vane pump was established with the help of MATLAB and Pro/E. The kinematic analysis helped in developing kinematic equations for cylindrical vane pumps and proved the effectiveness of the structural design. The maximum inaccuracy error of the computational fluid dynamics (CFD) model was 5.7% compared with the experimental results, and the CFD results show that the structure of the pump was reasonable. An experimental test bench was developed, and the results were in excellent agreement with the numerical results of CFD. The experimental results show that the cylindrical vane pump satisfied the three-element design of a positive-displacement pump and the trend of changes in efficiency was the same for all types of efficiency under different operating conditions. Furthermore, the volumetric efficiency presented a nonlinear positive correlation with increased rotational velocity, the mechanical efficiency showed a nonlinear negative correlation, and the total efficiency first increased and then decreased. When the rotational velocity was 1.33[Formula: see text] and the discharge pressure was 0.68[Formula: see text], the total efficiency reached its maximum value.

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