Electrochemical Profile and Antioxidant Potential of Gallic Acid in Presence of Ascorbic Acid

2018 ◽  
pp. 171-180
Author(s):  
Dulce A. Flores-Maltos ◽  
Cristobal N. Aguilar ◽  
Ruth E. Belmares ◽  
Raúl Rodríguez ◽  
L. V. Rodríguez-Duran ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gallic Acid ◽  
Antioxidant Potential

scholarly journals Aspergillus oryzae Fermented Rice Bran: A Byproduct with Enhanced Bioactive Compounds and Antioxidant Potential

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 70
Author(s):  
Sneh Punia ◽  
Kawaljit Singh Sandhu ◽  
Simona Grasso ◽  
Sukhvinder Singh Purewal ◽  
Maninder Kaur ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Gallic Acid ◽  
Aspergillus Oryzae ◽  
Rice Bran ◽  
Fungal Spores ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
The Impact

Rice bran (RB) is a byproduct of the rice industry (milling). For the fermentation process and to add value to it, RB was sprayed with fungal spores (Aspergillus oryzae MTCC 3107). The impact of fermentation duration on antioxidant properties was studied. Total phenolic content (TPC) determined using the Folin–Ciocalteu method, increased during fermentation until the 4th day. The antioxidant activity analyzed using the 2,2 Diphenyl–1′ picrylhydrazyl (DPPH) assay, total antioxidant activity (TAC), 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) assay, reducing power assay (RPA) and hydroxyl free radical scavenging activity (HFRSA) for fermented rice bran (FRB) were determined and compared to unfermented rice bran (URB). TAC, DPPH, ABTS+ and RPA of FRB increased till 4th day of fermentation, and then decreased. The specific bioactive constituents in extracts (Ethanol 50%) from FRB and URB were identified using high performance liquid chromatography (HPLC). HPLC confirmed a significant (p < 0.05) increase in gallic acid and ascorbic acid. On the 4th day of fermentation, the concentrations of gallic acid and ascorbic acid were 23.3 and 12.7 µg/g, respectively. The outcome of present investigation confirms that antioxidant potential and TPC of rice bran may be augmented using SSF.

scholarly journals Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Helena Abramovič ◽  
Blaž Grobin ◽  
Nataša Poklar Ulrih ◽  
Blaž Cigić
Keyword(s):  
Ascorbic Acid ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Sulfonic Acid ◽  
Epigallocatechin Gallate ◽  
The Other ◽  
Oh Groups ◽  
Standard Compound

Trolox, gallic acid, chlorogenic acid, caffeic acid, catechin, epigallocatechin gallate, and ascorbic acid are antioxidants used as standards for reaction with chromogenic radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH⋅) and 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS⋅+), and Folin–Ciocalteu (FC) reagent. The number of exchanged electrons has been analyzed as function of method and solvent. A majority of compounds exchange more electrons in FC assay than in ABTS and DPPH assays. In reaction with chromogenic radicals, the largest number of electrons was exchanged in buffer (pH 7.4) and the lowest reactivity was in methanol (DPPH) and water (ABTS). At physiological pH, the number of exchanged electrons of polyphenols exceeded the number of OH groups, pointing to the important contribution of partially oxidized antioxidants, formed in the course of reaction, to the antioxidant potential. For Trolox, small impact on the number of exchanged electrons was observed, confirming that it is more suitable as a standard compound than the other antioxidants.

scholarly journals HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation

2019 ◽  
Vol 27 (7) ◽  
pp. 975-980 ◽  
Author(s):  
Prawez Alam ◽  
Y.T. Kamal ◽  
Saleh Ibrahim Alqasoumi ◽  
Ahmed Ibrahim Foudah ◽  
Mohammed Hamed Alqarni ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gallic Acid ◽  
Simultaneous Determination ◽  
Pomegranate Juice ◽  
Herbal Formulation ◽  
Hptlc Method

scholarly journals Effects of mixtures of gallic acid, soya lecithin or catechin and ascorbic acid on the thermal decomposition of tocopherol in vegetable oils.

1991 ◽  
Vol 44 (1) ◽  
pp. 45-50
Author(s):  
Goro KAJIMOTO ◽  
Mariko SHIBATA ◽  
Yuki KANOMI ◽  
Hiromi YOSHIDA ◽  
Akira SHIBAHARA
Keyword(s):  
Thermal Decomposition ◽  
Ascorbic Acid ◽  
Gallic Acid ◽  
Vegetable Oils ◽  
Soya Lecithin

Thermal stability, antioxidant activity, and photo-oxidation of natural polyphenols

2014 ◽  
Vol 68 (1) ◽  
Author(s):  
Irina Volf ◽  
Ioana Ignat ◽  
Mariana Neamtu ◽  
Valentin Popa
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Gallic Acid ◽  
Vanillic Acid ◽  
Grape Seeds ◽  
Photo Oxidation ◽  
Degradation Rates ◽  
Ultraviolet C ◽  
Uv C

AbstractThe thermal stability (60°C, 80°C, 100°C), antioxidant activity, and ultraviolet C light (UV-C) stability of standard polyphenols solutions (catechin, gallic acid, and vanillic acid) and of vegetal extracts from spruce bark and grape seeds were investigated. Exposure of the standard solutions and vegetal extracts to high temperatures revealed that phenolic compounds were also relatively stable (degradations ranged from 15 % to 30 % after 4 h of exposure). The highest antioxidant activity was obtained for ascorbic acid and gallic acid followed by catechin and caffeic acid and the grape seeds. The results show that, after 3 h of UV-C exposure, approximately 40 % of vanillic acid, 50 % of gallic acid, and 83 % of catechin were removed. Similar degradation rates were observed for vegetal extracts, with the exception of the degradation of catechin (40 %) from grape seeds. In addition, the photo-oxidation of polyphenols in the presence of food constituents such as citric acid, ascorbic acid, sodium chloride, and sodium nitrate was assessed.

scholarly journals Simultaneous Identification of Phenolic Compound from the Honey of Stingless Bee by Using HPLC

2019 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Asma Nisar ◽  
Awang Bono ◽  
Hina Ahmad ◽  
Ambreen Lateef ◽  
Maham Mushtaq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gallic Acid ◽  
Royal Jelly ◽  
Antioxidant Properties ◽  
Reversed Phase ◽  
Hplc Method ◽  
Stingless Bee ◽  
Reversed Phase Hplc ◽  
Therapeutic Uses ◽  
Simultaneous Identification

Apitherapy is a branch of alternative medicine that uses honey bee products including honey, propolis, pollen, bee venom, and royal jelly. Stingless bee honey reportedly has many medicinal and therapeutic uses and excellent potency. This study aimed to identify the phenolic compounds using a fast and specific reversed-phase HPLC method in the extract of stingless bee honey. A magnetic stirrer was used for the pretreatment process of a sample with methanol at a temperature of 50°C for 40 min, followed by separation on a column size of 250 mm x 4.6 mm (5μm) hypersil gold-C18 (Thermo Electron Corporation) with water–methanol–acetonitrile (45:40:15 v/v/v) containing acetic acid 1.0% as a mobile phase. A 254-nm wavelength was used to detect the extract. The standard retention times of the gallic acid, rutin, ascorbic acid, quercetin, and kaempferol were found to be at 2.610, 2.875, 3.150, 5.789, and 8.983 min, respectively. The existence of gallic acid, rutin, ascorbic acid, kaempferol, and quercetin in the stingless bee honey extract was found to match according to the standard retention time. In the stingless bee honey, the retention times of gallic acid, rutin, ascorbic acid, quercetin, and kaempferol were found to be at 2.613, 2.866, 3.157, 5.790, and 8.966 min, respectively. In the stingless bee honey, the amounts of gallic acid, rutin, ascorbic acid, quercetin, and kaempferol were 1.426%, 2.533%, 16.922%, 1.851%, and 13.773%, respectively. According to the results, it is concluded that stingless bee honey is rich in phenolic acids and flavonoid compounds that have strong antioxidant properties.

scholarly journals Exogenous Application of Ascorbic Acid Enhances the Antimicrobial and Antioxidant Potential of Ocimum sanctum L. Grown under Salt Stress

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Neelma Munir ◽  
Sheza A. Khilji ◽  
Maryam Shabir ◽  
Zahoor A. Sajid
Keyword(s):  
Antimicrobial Activity ◽  
Ascorbic Acid ◽  
Salt Stress ◽  
Defense Mechanisms ◽  
Nacl Stress ◽  
Antioxidant Potential ◽  
Ocimum Sanctum ◽  
Enzymatic Antioxidants ◽  
Abiotic Stress Response ◽  
Antioxidants Activity

Ocimum sanctum L. (Tulsi) is the most important medicinal plant that has antimicrobial, antioxidants, and anticarcinogenic effects on human health. Plants, when under stress, gather several antioxidants and osmoprotectants. The present work focuses on the abiotic stress response of Tulsi and its mitigation by the application of ascorbic acid. In addition to this, an enhancement of antioxidant and antimicrobial activity was also analyzed using ascorbic acid. During the present work, when plants were grown under NaCl stress and ascorbic acid (AA) was provided with foliar applications, it ascertained encouraging effects on growth; likewise, its effect remains stable under salinity stress. The enzymatic antioxidants activity showed a significant change in response to AA alone or in combination. The highest catalase activity was recorded in plants subjected to 0.5 mM AA in combination with 100 mM NaCl (0.65 units/mL of enzyme). Likewise, a similar trend was recorded for the superoxide dismutase activity of Tulsi plants. The highest activity of SOD was recorded in plants subjected to 0.5 mM AA in combination with 100 mM NaCl (66.1 units/mg of protein). Flavonoid content showed its highest amount (27.41 mg/g) when plants were treated with 0.5 mM AA + 100 mM NaCl while the highest phenolic content (1.88 mg/g) was analyzed in salt treated plants sprayed with 0.5 mM AA. In the case of antimicrobial activity, 0.5 mM AA treated plants gave the highest value for the Staphylococcus aureus as 2.15 cm and in Clostridium species was 2.1 cm in the plants treated with 1 mM AA alone. Hence, the findings of the present study may lead to the conclusion that AA has a significant role in defense mechanisms of plants in response to salt stress. Further, it enhances the antimicrobial and antioxidant potential of Tulsi plants grown under salt stress.

scholarly journals Evaluation of the antioxidant activity of various extracts of aerial parts of Cassia absus: An in-vitro techniques

2020 ◽  
Vol 11 (2) ◽  
pp. 1545-1550
Author(s):  
Mythri M ◽  
Sanal Dev K T ◽  
Kottai Muthu A
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Potential ◽  
In Vitro Techniques ◽  
Aerial Parts ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
The Difference

Cassia absus (Linn)Cassia absus(Linn) (family Fabaceae ) is generally known as “chaksu ” inan ayurvedic traditional system.The current study,aerial parts of different concentrates(Pet.ether, ethyl acetate and methanol) of Cassia absus, was evaluated for its in-vitro antioxidant potential byDiphenylpicrylhydrazyl radical,nitric oxide activity andtotal antioxidant activitytaking ascorbate as the standardfor all the three methods. The IC50 value was originated that methanolic concentrates of Cassia absusmore efficient inDiphenylpicrylhydrazyl radical,nitric oxide activity, total antioxidant activitycompared EA&PEconcentrates.The methanolic concentrates of Cassia absus& ascorbic acid exhibited antioxidant potential possessing IC50230µg/ml &130µg/ml (Nitric oxide). 205µg/ml &57µg/ml (total antioxidant),195µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical)respectively. The difference in the scavenging potential of the extracts can be due to variation in the percentage of bioactive compounds present in different solvents. Invitroantioxidant studiesobviouslyshow the methanolic concentrates of Cassia absushave better antioxidant activity. This result indicates that aerial parts of methanolic concentratesCassia absuscould serve as a natural antioxidant, which may be useful in preventfree radical-induced diseases.

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