Population Structure and Diversity of Banana Bunchy Top Virus and Banana Bract Mosaic Virus

Plant Viruses ◽  
2018 ◽  
pp. 149-170
Author(s):  
R. Selvarajan ◽  
V. Balasubramanian ◽  
C. Anuradha
Keyword(s):  
Population Structure ◽  
Mosaic Virus ◽  
Banana Bunchy Top Virus ◽  
Banana Bract Mosaic Virus

scholarly journals Prediction and experimental confirmation of banana bract mosaic virus encoding miRNAs and their targets

ExRNA ◽  
2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Ramamoorthy Sankaranarayanan ◽  
Sankara Naynar Palani ◽  
Abhishek Kumar ◽  
Punitha Selvakumar A. S. ◽  
Jebasingh Tennyson
Keyword(s):  
Mosaic Virus ◽  
Experimental Confirmation ◽  
Banana Bract Mosaic Virus

Occurrence of Banana bract mosaic virus on cardamom

2011 ◽  
Vol 40 (1) ◽  
pp. 77-85 ◽  
Author(s):  
A. Siljo ◽  
A. I. Bhat ◽  
C. N. Biju ◽  
M. N. Venugopal
Keyword(s):  
Mosaic Virus ◽  
Banana Bract Mosaic Virus

scholarly journals Genetic variability and population structure of the New World begomovirus Euphorbia yellow mosaic virus

2017 ◽  
Vol 98 (6) ◽  
pp. 1537-1551 ◽  
Author(s):  
Talita Bernardon Mar ◽  
César Augusto Diniz Xavier ◽  
Alison Talis Martins Lima ◽  
Angélica Maria Nogueira ◽  
José Cleydson Ferreira Silva ◽  
...  
Keyword(s):  
Population Structure ◽  
Genetic Variability ◽  
Mosaic Virus ◽  
New World ◽  
Yellow Mosaic Virus

Evidence of seed transmission of Banana bract mosaic virus in Musa synthetic diploid H-201, a possible threat to banana breeding

2020 ◽  
Vol 156 (4) ◽  
pp. 1181-1187 ◽  
Author(s):  
Ramasamy Selvarajan ◽  
Veluswamy Balasubramanian ◽  
Perumal Priyanka ◽  
Ravikumar Manohar Jebakumar ◽  
Kanichelvam Prasanya Selvam ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Seed Transmission ◽  
Banana Bract Mosaic Virus

scholarly journals Recombinant Coat Protein of Banana Bract Mosaic Virus as a Potential Antigen for Serological Detection of the Virus

2020 ◽  
pp. 47-60
Author(s):  
Darsana Dilip ◽  
Vimi Louis ◽  
Pallavi Sabharwal ◽  
H. S. Savithri ◽  
P. M. Namitha ◽  
...  
Keyword(s):  
Coat Protein ◽  
Fluorescence Spectroscopy ◽  
Mosaic Virus ◽  
Gene Segment ◽  
Mosaic Disease ◽  
Polyclonal Antiserum ◽  
Expression Vectors ◽  
Affinity Column ◽  
Banana Bract Mosaic Virus

Banana bract mosaic disease caused by Banana bract mosaic potyvirus (BBrMV) is reported to instigate heavy loss in banana and plantain across Asia. Almost all the cultivars of banana succumb to the disease resulting in malformed bunches weighing less than half of normal ones. In the current study the coat protein (CP) gene segment present at the 3’ terminal region of the viral genome amplified by RT-PCR was cloned into expression vectors, pRSET-C and pGEX-4T-2 to use it for raising polyclonal antiserum which in turn will aid in developing assays to detect the virus. Recombinant BBrMV CP (rCP) in pRSET-C when expressed was insoluble whereas, it was in the soluble fraction when expressed from pGEX-4T-2. The GST-fusion protein was purified by GSH sepharose affinity column chromatography and western blot analysis was performed using anti GST antibodies. 360 µg/ml of protein was purified from 1 l of culture. The GST tag was cleaved from the purified protein by incubation with thrombin at 25°C overnight.  The rCP was characterized using ultracentrifugation, fluorescence spectroscopy and electron microscopy. The tagless monomer failed to assemble to virus like particles (VLPs) in vitro which was substantiated by fluorescence spectroscopy. This study will be first step towards deciphering structure and functions of Banana bract mosaic virus coat protein.

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