Preparation of axenic cultures in Ulva (Chlorophyta)

2018 ◽  
pp. 159-171 ◽  
Author(s):  
Gianmaria Califano ◽  
Thomas Wichard
Keyword(s):  
Axenic Cultures

Peroxidase Localization in Hartmanella Trophozoites

1971 ◽  
Vol 29 ◽  
pp. 346-347 ◽  
Author(s):  
George E. Childs ◽  
Joseph H. Miller
Keyword(s):  
Hydrogen Peroxide ◽  
Ultrastructural Localization ◽  
Pathogenic Strain ◽  
Oxidative Enzymes ◽  
Differential Centrifugation ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
The Subject ◽  
Axenic Cultures

Biochemical and differential centrifugation studies have demonstrated that the oxidative enzymes of Acanthamoeba sp. are localized in mitochondria and peroxisomes (microbodies). Although hartmanellid amoebae have been the subject of several electron microscopic studies, peroxisomes have not been described from these organisms or other protozoa. Cytochemical tests employing diaminobenzidine-tetra HCl (DAB) and hydrogen peroxide were used for the ultrastructural localization of peroxidases of trophozoites of Hartmanella sp. (A-l, Culbertson), a pathogenic strain grown in axenic cultures of trypticase soy broth.

An efficient method to obtain axenic cultures of Alexandrium tamarense—a PSP-producing dinoflagellate

2007 ◽  
Vol 69 (3) ◽  
pp. 425-430 ◽  
Author(s):  
JianQiang Su ◽  
XiaoRu Yang ◽  
TianLing Zheng ◽  
HuaSheng Hong
Keyword(s):  
Efficient Method ◽  
Alexandrium Tamarense ◽  
Axenic Cultures

Production of axenic cultures of algae by an osmotic method

Phycologia ◽  
1982 ◽  
Vol 21 (3) ◽  
pp. 408-410 ◽  
Author(s):  
L. M. Brown
Keyword(s):  
Axenic Cultures

Colony initiation in flax rust in axenic culture: involvement of a volatile factor

1979 ◽  
Vol 57 (23) ◽  
pp. 2657-2662 ◽  
Author(s):  
Rosalinda Boasson ◽  
Michael Shaw
Keyword(s):  
Carbon Dioxide ◽  
Axenic Culture ◽  
Conclusive Evidence ◽  
Culture Flask ◽  
Melampsora Lini ◽  
Air Space ◽  
Lag Period ◽  
Axenic Cultures ◽  
Incubation Temperatures

In axenic cultures of flax rust (Melampsora lini) colonies are initiated after a lag period of 12–20 days, depending partly on incubation temperatures. Colony initiation is completely inhibited by removal of a volatile factor which is absorbed by KOH in the air space of the culture flask. The fungus remains sensitive to this inhibition for 8–10 days, i.e., until shortly before visible colonies would normally have developed. While in the presence of KOH, the fungus is not killed; cultures grow normally after removal of the KOH.Although conclusive evidence must await further work, the available data strongly suggest that carbon dioxide is responsible for this effect.

Natural programmed cell death inT. cruziepimastigotes maintained in axenic cultures

2008 ◽  
Vol 105 (3) ◽  
pp. 688-698 ◽  
Author(s):  
V. Jimenez ◽  
R. Paredes ◽  
M.A. Sosa ◽  
N. Galanti
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Axenic Cultures

scholarly journals The use of antibiotics to obtain axenic cultures of algae

1973 ◽  
Vol 8 (2) ◽  
pp. 185-196 ◽  
Author(s):  
A.K. Jones ◽  
Muriel E. Rhodes ◽  
Susan C. Evans
Keyword(s):  
Axenic Cultures

5-Bromodeoxyuridine-induced formation of virus-like particles in Naegleria gruberi EGs

1977 ◽  
Vol 26 (1) ◽  
pp. 359-371
Author(s):  
F.L. Schuster ◽  
J.S. Clemente
Keyword(s):  
Food Source ◽  
Virus Like Particles ◽  
Culture Cells ◽  
Mammalian Cell Lines ◽  
Tissue Culture Cells ◽  
Excess Thymidine ◽  
Axenic Cultures ◽  
Future Work ◽  
Living Bacteria ◽  
Naegleria Gruberi

Exposure of axenic cultures of the amoeboflagellate Naegleria gruberi EGs to the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) resulted in the induction of virus-like particles (VLP) and various structures associated with their development and presumed transmission. Previously, VLP induction could be accomplished only by growing amoebae in the presence of living bacteria as a food source. Addition of excess thymidine along with BrdU did not block induction of particles. This account demonstrates that the EGs-VLP system responded to BrdU as do a number of mammalian cell lines harbouring latent viruses, and provides the basis for future work on the infectivity of the VLPs for other amoebae as well as tissue culture cells.

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