Metabolomics of intra- and extracellular metabolites from micro- and macroalgae using GC–MS and LC–MS

2018 ◽  
pp. 279-300
Author(s):  
Constanze Kuhlisch ◽  
Gianmaria Califano ◽  
Thomas Wichard ◽  
Georg Pohnert
Keyword(s):  
Extracellular Metabolites

Homoserine Lactones, Methyl Oligohydroxybutyrates, and Other Extracellular Metabolites of Macroalgae-Associated Bacteria of theRoseobacterClade: Identification and Functions

ChemBioChem ◽  
2015 ◽  
Vol 16 (14) ◽  
pp. 2094-2107 ◽  
Author(s):  
Lisa Ziesche ◽  
Hilke Bruns ◽  
Marco Dogs ◽  
Laura Wolter ◽  
Florian Mann ◽  
...  
Keyword(s):  
Associated Bacteria ◽  
Homoserine Lactones ◽  
Extracellular Metabolites

scholarly journals Spontaneous Gac Mutants of Pseudomonas Biological Control Strains: Cheaters or Mutualists?

2011 ◽  
Vol 77 (20) ◽  
pp. 7227-7235 ◽  
Author(s):  
William W. Driscoll ◽  
John W. Pepper ◽  
Leland S. Pierson ◽  
Elizabeth A. Pierson
Keyword(s):  
Alternative Hypothesis ◽  
Natural Populations ◽  
Regulatory System ◽  
Wild Type ◽  
Content Type ◽  
The Public ◽  
Extracellular Metabolites ◽  
Mutualistic Interaction ◽  
Alternative Hypotheses

ABSTRACTBacteria rely on a range of extracellular metabolites to suppress competitors, gain access to resources, and exploit plant or animal hosts. The GacS/GacA two-component regulatory system positively controls the expression of many of these beneficial external products in pseudomonad bacteria. Natural populations often contain variants with defective Gac systems that do not produce most external products. These mutants benefit from a decreased metabolic load but do not appear to displace the wild type in nature. How could natural selection maintain the wild type in the presence of a mutant with enhanced growth? One hypothesis is that Gac mutants are “cheaters” that do not contribute to the public good, favored within groups but selected against between groups, as groups containing more mutants lose access to ecologically important external products. An alternative hypothesis is that Gac mutants have a mutualistic interaction with the wild type, so that each variant benefits by the presence of the other. In the biocontrol bacteriumPseudomonas chlororaphisstrain 30-84, Gac mutants do not produce phenazines, which suppress competitor growth and are critical for biofilm formation. Here, we test the predictions of these alternative hypotheses by quantifying interactions between the wild type and the phenazine- and biofilm-deficient Gac mutant within growing biofilms. We find evidence that the wild type and Gac mutants interact mutualistically in the biofilm context, whereas a phenazine-defective structural mutant does not. Our results suggest that the persistence of alternative Gac phenotypes may be due to the stabilizing role of local mutualistic interactions.

scholarly journals Antimicrobial activity of extracellular metabolites from antagonistic bacteria isolated from potato (Solanum phureja) crops

2014 ◽  
Vol 40 (3) ◽  
pp. 212-220 ◽  
Author(s):  
Sinar David Granada García ◽  
Antoni Rueda Lorza ◽  
Carlos Alberto Peláez
Keyword(s):  
Antimicrobial Activity ◽  
Cyclic Peptide ◽  
Biological Activities ◽  
Pathogenic Fungi ◽  
Antagonistic Activity ◽  
Solanum Phureja ◽  
Dual Culture ◽  
Crude Extracts ◽  
Extracellular Metabolites

Microorganisms for biological control are capable of producing active compounds that inhibit the development of phytopathogens, constituting a promising tool toob tain active principles that could replace synthetic pesticides. This study evaluatedtheability of severalpotentialbiocontrol microorganismsto produce active extracellular metabolites. In vitro antagonistic capability of 50 bacterial isolates from rhizospheric soils of "criolla" potato (Solanum phureja) was tested through dual culture in this plant with different plant pathogenic fungi and bacteria. Isolates that showed significantly higher antagonistic activity were fermented in liquid media and crude extracts from the supernatants had their biological activities assessed by optical density techniques. Inhibitory effecton tested pathogens was observed for concentrations between 0.5% and 1% of crude extracts. There was a correlation between the antimicrobial activity of extracts and the use of nutrient-rich media in bacteria fermentation. Using a bioguided method, a peptidic compound, active against Fusarium oxysporum, was obtained from the 7ANT04 strain (Pyrobaculum sp.). Analysis by nuclear magnetic resonance and liquid chromatography coupled to mass detector evidenced an 11-amino acid compound. Bioinformatic software using raw mass data confirmed the presence of a cyclic peptide conformed by 11 mostly non-standard amino acids.

scholarly journals Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

2019 ◽  
Vol 9 (4) ◽  
pp. 720 ◽  
Author(s):  
Galina Gayda ◽  
Olha Demkiv ◽  
Nataliya Stasyuk ◽  
Roman Serkiz ◽  
Maksym Lootsik ◽  
...  
Keyword(s):  
Green Synthesis ◽  
Metallic Nanoparticles ◽  
Alcohol Oxidase ◽  
Inorganic Ions ◽  
Special Role ◽  
Graphite Electrodes ◽  
Fluorescent Markers ◽  
Extracellular Metabolites ◽  
Very High

Novel nanomaterials, including metallic nanoparticles obtained via green synthesis (gNPs), have a great potential for application in biotechnology, industry and medicine. The special role of gNPs is related to antibacterial agents, fluorescent markers and carriers for drug delivery. However, application of gNPs for construction of amperometric biosensors (ABSs) is not well documented. The aim of the current research was to study potential advantages of using gNPs in biosensorics. The extracellular metabolites of the yeast Ogataea polymorpha were used as reducing agents for obtaining gNPs from the corresponding inorganic ions. Several gNPs were synthesized, characterized and tested as enzyme carriers on the surface of graphite electrodes (GEs). The most effective were Pd-based gNPs (gPdNPs), and these were studied further and applied for construction of laccase- and alcohol oxidase (AO)-based ABSs. AO/GE, AO-gPdNPs/GE, laccase/GE and laccase-gPdNPs/GE were obtained, and their analytical characteristics were studied. Both gPdNPs-modified ABSs were found to have broader linear ranges and higher storage stabilities than control electrodes, although they are less sensitive toward corresponding substrates. We thus conclude that gPdNPs may be promising for construction of ABSs for enzymes with very high affinities to their substrates.

scholarly journals 13C labeling dynamics of intra- and extracellular metabolites in CHO suspension cells

2013 ◽  
Vol 7 (S6) ◽  
Author(s):  
Judith Wahrheit ◽  
Averina Nicolae ◽  
Elmar Heinzle
Keyword(s):  
Suspension Cells ◽  
13C Labeling ◽  
Extracellular Metabolites

scholarly journals Self-establishing communities enable cooperative metabolite exchange in a eukaryote

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Kate Campbell ◽  
Jakob Vowinckel ◽  
Michael Mülleder ◽  
Silke Malmsheimer ◽  
Nicola Lawrence ◽  
...  
Keyword(s):  
Growth Parameters ◽  
Exchange Interactions ◽  
Supporting Cell ◽  
Nucleotide Metabolism ◽  
Dependent Manner ◽  
Metabolic Function ◽  
Wild Type ◽  
Progressive Loss ◽  
Extracellular Metabolites ◽  
Metabolite Exchange

Metabolite exchange among co-growing cells is frequent by nature, however, is not necessarily occurring at growth-relevant quantities indicative of non-cell-autonomous metabolic function. Complementary auxotrophs of Saccharomyces cerevisiae amino acid and nucleotide metabolism regularly fail to compensate for each other's deficiencies upon co-culturing, a situation which implied the absence of growth-relevant metabolite exchange interactions. Contrastingly, we find that yeast colonies maintain a rich exometabolome and that cells prefer the uptake of extracellular metabolites over self-synthesis, indicators of ongoing metabolite exchange. We conceived a system that circumvents co-culturing and begins with a self-supporting cell that grows autonomously into a heterogeneous community, only able to survive by exchanging histidine, leucine, uracil, and methionine. Compensating for the progressive loss of prototrophy, self-establishing communities successfully obtained an auxotrophic composition in a nutrition-dependent manner, maintaining a wild-type like exometabolome, growth parameters, and cell viability. Yeast, as a eukaryotic model, thus possesses extensive capacity for growth-relevant metabolite exchange and readily cooperates in metabolism within progressively establishing communities.

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