scholarly journals Mitochondria Structure and Position in the Local Control of Calcium Signals in Smooth Muscle Cells

2018 ◽  
pp. 173-190
Author(s):  
John G. McCarron ◽  
Christopher Saunter ◽  
Calum Wilson ◽  
John M. Girkin ◽  
Susan Chalmers
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Local Control ◽  
Muscle Cells ◽  
Calcium Signals

scholarly journals 12-Lipoxygenase products modulate calcium signals in vascular smooth muscle cells.

Hypertension ◽  
1992 ◽  
Vol 20 (2) ◽  
pp. 138-143 ◽  
Author(s):  
F Saito ◽  
M T Hori ◽  
Y Ideguchi ◽  
M Berger ◽  
M Golub ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Calcium Signals ◽  
Lipoxygenase Products ◽  
12 Lipoxygenase

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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