Calcium Sensitization in Smooth Muscle Involving Regulation of Myosin Light Chain Phosphatase Activity

2018 ◽  
pp. 123-153 ◽  
Author(s):  
Justin A. MacDonald ◽  
Michael P. Walsh ◽  
William C. Cole
Keyword(s):  
Smooth Muscle ◽  
Phosphatase Activity ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Myosin Light Chain Phosphatase ◽  
Calcium Sensitization

scholarly journals Regulation of Myosin Light-Chain Phosphatase Activity to Generate Airway Smooth Muscle Hypercontractility

2020 ◽  
Vol 11 ◽  
Author(s):  
Mayra D. Álvarez-Santos ◽  
Marisol Álvarez-González ◽  
Samuel Estrada-Soto ◽  
Blanca Bazán-Perkins
Keyword(s):  
Smooth Muscle ◽  
Phosphatase Activity ◽  
Airway Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Myosin Light Chain Phosphatase

scholarly journals Regions of the 110-kDa Regulatory Subunit M110 Required for Regulation of Myosin-Light-Chain-Phosphatase Activity in Smooth Muscle

1996 ◽  
Vol 239 (2) ◽  
pp. 326-332 ◽  
Author(s):  
Philippe Gailly ◽  
Xuqiong Wu ◽  
Timothy A. J. Haystead ◽  
Andrew P. Somlyo ◽  
Patricia T. W. Cohen ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Phosphatase Activity ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Regulatory Subunit ◽  
Myosin Light Chain Phosphatase

scholarly journals Arachidonic acid inhibits myosin light chain phosphatase and sensitizes smooth muscle to calcium.

1992 ◽  
Vol 267 (30) ◽  
pp. 21492-21498
Author(s):  
M.C. Gong ◽  
A Fuglsang ◽  
D Alessi ◽  
S Kobayashi ◽  
P Cohen ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Myosin Light Chain Phosphatase

Modulation of uterine resistance artery lumen diameter by calcium and G protein activation during pregnancy

1994 ◽  
Vol 267 (3) ◽  
pp. H952-H961 ◽  
Author(s):  
G. D'Angelo ◽  
G. Osol
Keyword(s):  
Smooth Muscle ◽  
G Protein ◽  
G Proteins ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Lumen Diameter ◽  
Maximal Response ◽  
Adrenergic Stimulation ◽  
Myosin Light Chain Phosphatase ◽  
Arterial Lumen

The purpose of this study was to determine whether the increased sensitivity of uterine resistance arteries from late pregnant (LP) rats to alpha-adrenergic stimulation is due to an alteration in the fundamental relationship between cytosolic calcium (Ca2+) and arterial lumen diameter. Uterine arcuate arteries were permeabilized with Staphylococcus aureus alpha-toxin under optimal conditions and constricted to varying degrees with discrete Ca2+ concentrations at a distending pressure of 50 mmHg. Arterial segments from nonpregnant (NP) and LP rats exhibited similar Ca2+/lumen diameter characteristics. Ca2+ (0.1 microM) produced appreciable constriction, and lumen diameter decreased steeply between 0.175 and 0.25 microM Ca2+; maximal responses were attained with 0.5 microM Ca2+. Activation of guanine nucleotide binding proteins (G proteins) with guanosine 5'-triphosphate (GTP; 1-100 microM), as reportedly occurs during alpha-adrenergic stimulation, potentiated the Ca(2+)-induced constriction by 121 and 79% in arteries from LP and NP rats, respectively. No significant differences between the two animal groups were noted. Guanosine 5'-O-(gamma-thiotriphosphate) (GTP gamma S; 0.1-10 microM), a nonhydrolyzable analogue of GTP, effected a larger potentiating effect over that maximal response caused by GTP in arteries from NP rats. Ca(2+)- and Ca2+/GTP-induced constrictions were more potently reversed by guanosine 5'-O-(beta-thiodiphosphate) (GDP beta S)., a competitive inhibitor of GTP, in arteries from NP rats. These data suggest that pregnancy-induced increases in sensitivity to alpha-adrenergic stimulation may be related to altered G protein cycling rates, such that G proteins in smooth muscle cells in arcuate arteries from NP rats are more susceptible to deactivation. Alternatively, consistent with the model of G protein-mediated inhibition of myosin light chain phosphatase, myosin light chain phosphatase activity may be enhanced in uterine vascular smooth muscle from NP rats relative to that from LP rats.

scholarly journals Recombinant Small Subunit of Smooth Muscle Myosin Light Chain Phosphatase

2001 ◽  
Vol 276 (36) ◽  
pp. 34318-34322 ◽  
Author(s):  
Knut Langsetmo ◽  
Walter F. Stafford ◽  
Katsuhide Mabuchi ◽  
Terence Tao
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Small Subunit ◽  
Smooth Muscle Myosin ◽  
Myosin Light Chain Phosphatase ◽  
Muscle Myosin
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