Development of a passive bioremediation process based on sulfate-reduction to treat arsenic-containing acidic mine water

Author(s):  
F Battaglia-Brunet ◽  
C Joulian ◽  
C Casiot
2019 ◽  
Author(s):  
Charles N. Alpers ◽  
◽  
Kate M. Campbell ◽  
D. Kirk Nordstrom ◽  
Thomas P. Chapin ◽  
...  
Keyword(s):  

2017 ◽  
Vol 262 ◽  
pp. 398-402
Author(s):  
Nadja Eisen ◽  
Felix Straube ◽  
Simone Schopf ◽  
Michael Schlömann

In the course of the decline of high-grade ore deposits, new effective and eco-friendly bioleaching techniques are of interest. In-situ leaching is an auspicious method, but composition of leaching community should be adapted to the respective external conditions and the ore material. In this study several sulfidic minerals were inoculated into acidic mine water of a mine in eastern Germany, housing members of well-known iron oxidizing bacteria like Acidithiobacillus, Leptospirillum and Ferrovum. The attachment tests were performed in batch and in a continuous way at different temperatures. The analysis of the extracted DNA from adhered cells showed an enrichment of Ferrovum spp. on chalcopyrite surface under in-situ conditions at 11°C. For laboratory batch conditions an accumulation of Leptospirillum spp. was detected for adhered cells probably due to the changes of the physicochemical parameter of the mine water. In more detailed analyses we aim to elucidate possible preferential attachment of the mine water community members to certain minerals.


2010 ◽  
Vol 25 (8) ◽  
pp. 1107-1119 ◽  
Author(s):  
Christian Wisskirchen ◽  
Bernhard Dold ◽  
Kurt Friese ◽  
Jorge E. Spangenberg ◽  
Peter Morgenstern ◽  
...  

2013 ◽  
Vol 825 ◽  
pp. 392-395 ◽  
Author(s):  
Robert Klein ◽  
Michael Schlömann ◽  
Yun Zeng ◽  
Bernd Wacker ◽  
Franz Glombitza ◽  
...  

Treatment of acidic Fe (II)- and sulfate-rich mine waters represents a major problem in many areas of the world. Therefore, a process was developed which utilises naturally occurring sulfate-reducing microorganisms for the elimination of sulfate and of part of the acidity from the acidic mine water. In order to improve the performance of this biological sulfate reduction process an in-depth analysis of the microbial diversity and activity in dependence of the hydraulic retention time (HRT) and other process parameters used to run the bioreactors was undertaken. This comparison demonstrated a positive correlation between shorter HRT and increasing sulfate reduction rates. The improvement in performance with decreasing HRT was paralleled by an increase of the total enzymatic activity (measured as hydrolase activity) of the microbial community and of the biomass (measured as protein concentration) in the bioreactors. A partial taxonomic identification of the microbial community in the bioreactors was achieved via nucleotide sequence analysis of a clone library of PCR-amplified 16S rRNA gene fragments prepared from a sample of the microbial biofilm in the bioreactor. Additionally, the genetic fingerprint technique T-RFLP was used to assess temporal changes of the microbial community in the biofilm within the reactor.


2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
W. Yang ◽  
A. M. Marshall ◽  
D. Wanatowski ◽  
L. R. Stace

Many shaft collapses are related to the deterioration and failure of the masonry shaft lining materials. In modern mine shaft, concrete is widely used to provide support. To analyse shafts stability, the properties of the lining need to be well defined. The behaviour of masonry and concrete can be considerably affected by long-term exposure to harsh mine water. This paper presents a study which focuses on the weathering effects of mine water on lining materials (brick, mortar, and concrete). To reproduce the weathering process, samples were placed into solutions of potable water, artificial mine water, and a more aggressive mine-water solution for just less than one year. Four phases of laboratory tests were conducted throughout the time period to assess the degradation of mechanical properties of the lining materials. Particular attention is given to the degradation of material strength and stiffness. Results indicate that the harsh acidic mine water has pronounced detrimental effects on the strength and stiffness of mortar. The weathering process is shown to have the most significant effect on the stiffness of concrete and mortar. It is also shown that the use of mass loss as an index for evaluation of mechanical properties may not be appropriate.


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