Cellular Dynamics of the Primary Shoot and Root Meristem

2016 ◽  
pp. 199-219
Author(s):  
Lam Vu ◽  
Ive De Smet
Keyword(s):  
Root Meristem ◽  
Cellular Dynamics ◽  
Primary Shoot

Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry)

1975 ◽  
Vol 33 ◽  
pp. 562-563
Author(s):  
Arya K. Bal
Keyword(s):  
Root Meristem ◽  
Uranyl Acetate ◽  
Particulate Material ◽  
Ultrastructural Changes ◽  
Cell Organelles ◽  
Cytoplasmic Matrix ◽  
Golgi Membranes ◽  
Rubus Chamaemorus ◽  
Dense Substance ◽  
Ultrathin Sections

In the course of studies in the root meristem tissue of Rubus chamaemorus L. some important changes in the ultrastructural morphology were observed during the initiation of senescence at the end of the growing season.Root meristems were collected from naturally growing healthy populations of Cloudberry plants, and fixed in Karnovsky's mixture or in 2.5% glutaraldehyde in phosphate buffer. The samples were osmicated, dehydrated following usual methods and embedded in Epon. Ultrathin sections were stained in uranyl acetate and lead citrate.Figure 1 shows part of a dense cell in the meristem. The electron density of these cells is due to large amounts of a particulate material in the cytoplasmic matrix. The smallest particle seen in electron micrographs is about 40 A, although larger aggregates are also found, which remain randomly distributed in association with various cell organelles. Dense substance has been found associated with golgi membranes, proplastids, vacuoles and microtubules (Fig. 2).

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