scholarly journals Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge

2016 ◽  
pp. 297-320
Keyword(s):  
Quorum Sensing ◽  
Activated Sludge ◽  
Molecular Characterization ◽  
Membrane Bioreactor ◽  
Signal Molecules

scholarly journals Identification of Quorum-Sensing Signal Molecules and a Biosynthetic Gene in Alicycliphilus sp. Isolated from Activated Sludge

Sensors ◽  
2016 ◽  
Vol 16 (8) ◽  
pp. 1218 ◽  
Author(s):  
Tomohiro Morohoshi ◽  
Noriya Okutsu ◽  
Xiaonan Xie ◽  
Tsukasa Ikeda
Keyword(s):  
Quorum Sensing ◽  
Activated Sludge ◽  
Signal Molecules ◽  
Biosynthetic Gene

Selective enrichment and molecular characterization of a previously uncultured Nitrospira-like bacterium from activated sludge

2006 ◽  
Vol 8 (3) ◽  
pp. 405-415 ◽  
Author(s):  
Eva Spieck ◽  
Christine Hartwig ◽  
Iris McCormack ◽  
Frank Maixner ◽  
Michael Wagner ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Molecular Characterization ◽  
Selective Enrichment

scholarly journals AmiE, a NovelN-Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate Acinetobacter sp. Strain Ooi24

2014 ◽  
Vol 80 (22) ◽  
pp. 6919-6925 ◽  
Author(s):  
Seiji Ochiai ◽  
Sera Yasumoto ◽  
Tomohiro Morohoshi ◽  
Tsukasa Ikeda
Keyword(s):  
Quorum Sensing ◽  
Activated Sludge ◽  
High Performance ◽  
Genomic Library ◽  
Amide Bond ◽  
Signal Molecules ◽  
Content Type ◽  
Chromatography Analysis ◽  
Acylhomoserine Lactone ◽  
Acyl Chains

ABSTRACTMany Gram-negative bacteria useN-acyl-l-homoserine lactones (AHLs) as quorum-sensing signal molecules. We have reported thatAcinetobacterstrains isolated from activated sludge have AHL-degrading activity. In this study, we cloned theamiEgene as an AHL-degradative gene from the genomic library ofAcinetobactersp. strain Ooi24. High-performance liquid chromatography analysis revealed that AmiE functions as an AHL acylase, which hydrolyzes the amide bond of AHL. AmiE showed a high level of degrading activity against AHLs with long acyl chains but no activity against AHLs with acyl chains shorter than eight carbons. AmiE showed homology with a member of the amidases (EC 3.5.1.4) but not with any known AHL acylase enzymes. An amino acid sequence of AmiE from Ooi24 showed greater than 99% identities with uncharacterized proteins fromAcinetobacter ursingiiCIP 107286 andAcinetobactersp. strain CIP 102129, but it was not found in the draft or complete genome sequences of otherAcinetobacterstrains. The presence of transposase-like genes around theamiEgenes of these threeAcinetobacterstrains suggests thatamiEis transferred by a putative transposon. Furthermore, the expression of AmiE inPseudomonas aeruginosaPAO1 reduced AHL accumulation and elastase activity, which were regulated by AHL-mediated quorum sensing.

scholarly journals Molecular characterization of microbial community in nitrate-removing activated sludge

2002 ◽  
Vol 41 (2) ◽  
pp. 85-94 ◽  
Author(s):  
Han-Woong Lee ◽  
Soo-Youn Lee ◽  
Jin-Woo Lee ◽  
Jong-Bok Park ◽  
Eui-So Choi ◽  
...  
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Molecular Characterization

scholarly journals Molecular characterization of activated sludge from a seawater-processing wastewater treatment plant

2011 ◽  
Vol 4 (5) ◽  
pp. 628-642 ◽  
Author(s):  
Olga Sánchez ◽  
Laura Garrido ◽  
Irene Forn ◽  
Ramon Massana ◽  
Manuel Ignacio Maldonado ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Molecular Characterization ◽  
Wastewater Treatment Plant ◽  
Treatment Plant

Production and Degradation ofN-Acylhomoserine Lactone Quorum Sensing Signal Molecules in Bacteria Isolated from Activated Sludge

2013 ◽  
Vol 77 (12) ◽  
pp. 2436-2440 ◽  
Author(s):  
Seiji OCHIAI ◽  
Tomohiro MOROHOSHI ◽  
Ayane KURABEISHI ◽  
Masahiro SHINOZAKI ◽  
Haruka FUJITA ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Activated Sludge ◽  
Signal Molecules ◽  
Acylhomoserine Lactone
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