Effect of PGRS in Vitro Callus Culture for Production of Secondary Metabolites

2016 ◽  
pp. 211-217
Author(s):  
Jitendra Mittal ◽  
Madan Sharma ◽  
Abhijeet Singh ◽  
Amla Batra
Keyword(s):  
Secondary Metabolites ◽  
Callus Culture

scholarly journals Isolation of furanocoumarins from Pastinaca sativa L. callus culture

2014 ◽  
Vol 69 (3) ◽  
pp. 193-195 ◽  
Author(s):  
Halina Ekiert ◽  
Wanda Kisiel
Keyword(s):  
Secondary Metabolites ◽  
Callus Culture ◽  
Spectral Methods ◽  
Solid Medium ◽  
Intact Plant ◽  
Pastinaca Sativa ◽  
First Report ◽  
Callus Tissues ◽  
First Time

Four furanocoumarins: bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins) and sphondin (angular furanocoumarin) were isolated for the first time from callus tissues of <em>Pastinaca sativa</em> L.(<em>Apiaceae</em>) cultured in vitro on solid medium. The compounds were identified using spectral methods. They are well-known secondary metabolites of the intact plant. This is the first report on the isolation of sphondin from in vitro plant cultures.

The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica

2015 ◽  
Vol 122 (2) ◽  
pp. 331-339 ◽  
Author(s):  
Nasrin Pourebad ◽  
Rouhollah Motafakkerazad ◽  
Morteza Kosari-Nasab ◽  
Nader Farsad Akhtar ◽  
Ali Movafeghi
Keyword(s):  
Secondary Metabolites ◽  
Callus Culture ◽  
In Vitro Growth

scholarly journals Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of Solanum xanthocarpum Callus Cultures

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2201 ◽  
Author(s):  
Hazrat Usman ◽  
Muhammad Asad Ullah ◽  
Hasnain Jan ◽  
Aisha Siddiquah ◽  
Samantha Drouet ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Caffeic Acid ◽  
Blue Light ◽  
Callus Culture ◽  
Biological Activities ◽  
Callus Cultures ◽  
Anti Inflammatory ◽  
Methyl Caffeate ◽  
Solanum Xanthocarpum

Solanum xanthocarpum is considered an important traditional medicinal herb because of its unique antioxidant, and anti-diabetic, anti-aging, and anti-inflammatory potential. Because of the over exploitation linked to its medicinal properties as well as destruction of its natural habitat, S. xanthocarpum is now becoming endangered and its supply is limited. Plant in vitro culture and elicitation are attractive alternative strategies to produce biomass and stimulate biosynthesis of medicinally important phytochemicals. Here, we investigated the potential influence of seven different monochromatic light treatments on biomass and secondary metabolites accumulation in callus culture of S. xanthocarpum as well as associated biological activities of the corresponding extracts. Among different light treatments, highest biomass accumulation was observed in white light-treated callus culture. Optimum accumulation of total flavonoid contents (TFC) and total phenolic contents (TPC) were observed in callus culture kept under continuous white and blue light respectively than control. Quantification of phytochemicals through HPLC revealed that optimum production of caffeic acid (0.57 ± 0.06 mg/g DW), methyl-caffeate (17.19 mg/g ± 1.79 DW), scopoletin (2.28 ± 0.13 mg/g DW), and esculetin (0.68 ± 0.07 mg/g DW) was observed under blue light callus cultures. Compared to the classic photoperiod condition, caffeic acid, methyl-caffeate, scopoletin, and esculetin were accumulated 1.7, 2.5, 1.1, and 1.09-folds higher, respectively. Moreover, high in vitro cell free antioxidant, anti-diabetic, anti-aging, and anti-inflammatory activities were closely associated with the production of these secondary metabolites. These results clearly showed the interest to apply multispectral light as elicitor of in vitro callus cultures S. xanthocarpum to promote the production of important phytochemicals, and allow us to propose this system as an alternative for the collection of this endangered species from the wild.

Effect of PGRS in Vitro Callus Culture for Production of Secondary Metabolites

2017 ◽  
pp. 211-217
Author(s):  
Jitendra Mittal ◽  
Madan Mohan Sharma ◽  
Abhijeet Singh ◽  
Amla Batra
Keyword(s):  
Secondary Metabolites ◽  
Callus Culture

scholarly journals CALLUS AND CELL SUSPENSION CULTURE OF DROSERA BURMANNI VAHL FOR QUINONE PRODUCTION

2010 ◽  
Vol 13 (2) ◽  
pp. 53-61
Author(s):  
Phuong Ngo Diem Quach ◽  
Minh Thi Thanh Hoang ◽  
Thu Thi Hoang ◽  
Le Van Bui
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Callus Culture ◽  
Bioactive Compounds ◽  
Cell Suspension Culture ◽  
Hplc Analysis ◽  
Cell Biomass ◽  
Culture Process

Drosera burmanni Vahl, one of three Drosera species in Vietnam, has been successfully cultured in vitro. Our previous researchs have shown that extracts of Drosera burmanni Vahl contain bioactive compounds such as naphthoquinone, anthraquinone. To obtain cell biomass as well as increase secondary metabolites, callus and cell suspension culture of Drosera burmanni Vahl become extremely urgent. Therefore, in this study, we focused on building Drosera burmanni Vahl callus and suspension culture process to obtain quinone. Our results show that the most optimized medium for callus culture is Gamborg’s B5, saccharose 20g/l, casein 100 mg/l, PVP 1g/l. To induce callus culture, the best hormone’s concentration is 2,4-D 0,2 mg/l, NAA 0,2 mg/l. Growing callus and increasing cell biomass in suspension culture are the same culture type. The peak of growing phase is on 12th. HPLC analysis showed present of plumbagin, one of quinone bioactive compounds determined in Drosera species, on cultured cell suspension.

Effect of Nutrient Medium, Phytohormones and Elicitation Treatment on in-vitro Callus Culture of Bacopa monniera and Expression of Secondary Metabolites

2014 ◽  
Vol 4 (1) ◽  
pp. 13-17
Author(s):  
Aziz Ahmed ◽  
Mahfoozur Rahman ◽  
Tamboli E. Tajuddin ◽  
Tanwir Athar ◽  
Mhaveer Singh ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Callus Culture ◽  
Nutrient Medium ◽  
Bacopa Monniera

Synthesis of secondary metabolites in vitro

2015 ◽  
Author(s):  
V. Naumenko ◽  
B. Sorochynskyi ◽  
Ya. Blume
Keyword(s):  
Secondary Metabolites

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

Biological and In silico Studies on Synthetic Analogues of Tyrosine Betaine as Inhibitors of Neprilysin - A Drug Target for the Treatment of Heart Failure

2018 ◽  
Vol 24 (17) ◽  
pp. 1899-1904
Author(s):  
Daniel Fabio Kawano ◽  
Marcelo Rodrigues de Carvalho ◽  
Mauricio Ferreira Marcondes Machado ◽  
Adriana Karaoglanovic Carmona ◽  
Gilberto Ubida Leite Braga ◽  
...  
Keyword(s):  
Heart Failure ◽  
Secondary Metabolites ◽  
Structural Similarity ◽  
Structural Features ◽  
Major Constituent ◽  
Binding Modes ◽  
Starting Point ◽  
In Silico Studies ◽  
Nep Inhibition

Background: Fungal secondary metabolites are important sources for the discovery of new pharmaceuticals, as exemplified by penicillin, lovastatin and cyclosporine. Searching for secondary metabolites of the fungi Metarhizium spp., we previously identified tyrosine betaine as a major constituent. Methods: Because of the structural similarity with other inhibitors of neprilysin (NEP), an enzyme explored for the treatment of heart failure, we devised the synthesis of tyrosine betaine and three analogues to be subjected to in vitro NEP inhibition assays and to molecular modeling studies. Results: In spite of the similar binding modes with other NEP inhibitors, these compounds only displayed moderate inhibitory activities (IC50 ranging from 170.0 to 52.9 µM). However, they enclose structural features required to hinder passive blood brain barrier permeation (BBB). Conclusions: Tyrosine betaine remains as a starting point for the development of NEP inhibitors because of the low probability of BBB permeation and, consequently, of NEP inhibition at the Central Nervous System, which is associated to an increment in the Aβ levels and, accordingly, with a higher risk for the onset of Alzheimer's disease.

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