Autoimmunity against selenium transport in human sera

Author(s):  
Waldemar Minich ◽  
Andrea Schuette ◽  
Christian Schwiebert ◽  
Tim Welsink ◽  
Kostja Renko ◽  
...  
Keyword(s):  
ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 213-218
Author(s):  
Suresh Chand Kaushik ◽  
Sukhvir Singh ◽  
Purnima Srivastava ◽  
R. Rajendran

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.


1966 ◽  
Vol 53 (4) ◽  
pp. 673-680 ◽  
Author(s):  
Torsten Deckert ◽  
Kai R. Jorgensen

ABSTRACT The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales & Randle's (1963) immunoassay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pig and ox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal subjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non diabetic subjects and crystalline pig insulin.


1960 ◽  
Vol XXXV (III) ◽  
pp. 381-396 ◽  
Author(s):  
Sven Almqvist

ABSTRACT The sulfation factor (SF) activity of human sera has been estimated using a modification of the method of Daughaday et al. (1959). Each assay was statistically evaluated. The method had a mean precision of 0.14 and, used as an assay of GH of human serum, a sensitivity in three pituitary dwarfs of 0.1 to 0.6 μg of HGH/ml of serum. SF activity was found at all ages between 1 month and 75 years. There was a significantly lower mean SF activity below the age of half a year. Three cases of pituitary dwarfism had significantly low SF activities of sera. There was no significant difference between the SF activities of sera from untreated pituitary dwarfs and the sera from normal children below half a year of age. Dose-response curves with large volumes of sera from pituitary dwarfs and small volumes of sera from normal humans had the same slopes. Four mg of HGH prepared according to the method of Li & Papkoff (1956) resulted in a normal serum SF activity in each of the three dwarfs. A significant (P < 0.01) linear relationship was found between the concentration of SF activity of sera from these subjects and the logarithm of the dose of HGH given with dose levels of 1, 2 and 4 mg daily for three days. The decline of serum SF activity to the pre-treatment level following HGH in one dwarf suggested a half life not different from that indicated by others for growth hormone.


1967 ◽  
Vol 45 (4) ◽  
pp. 541-550 ◽  
Author(s):  
A. P. Gaunce ◽  
P. A. Anastassiadis

The distribution of hexosamine among the proteins of avian, bovine, porcine, and human blood sera was studied by electrophoresis on filter paper. Hexosamine was determined after direct hydrolysis of stained sections of the paper, followed by chromatography of hydrolysates on cationic resin. Some substantial and statistically significant differences in hexosamine and protein contents of the zones were found among species.


1989 ◽  
Vol 30 (5) ◽  
pp. 701-710 ◽  
Author(s):  
L Lagrost ◽  
P Gambert ◽  
S Meunier ◽  
P Morgado ◽  
J Desgres ◽  
...  
Keyword(s):  

Toxicology ◽  
2021 ◽  
pp. 152854
Author(s):  
Alistair C. Leitch ◽  
Ibrahim Ibrahim ◽  
Tarek M. Abdelghany ◽  
Alex Charlton ◽  
Clair Roper ◽  
...  

1975 ◽  
Vol 74 (1) ◽  
pp. 35-41 ◽  
Author(s):  
M. R. Hollingdale

SUMMARYA serological analysis of mycelial antigens ofThermoactinomyces vulgarisin immunodiffusion with human sera revealed five individual antigens. Three antigens were proteins, sensitive to pronase and soluble in phenol. Two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid.Latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies toT. vulgaris;the number of positive results detected by each test differed slightly, and a combination of the two tests detected the highest number. Counterimmunoelectrophoresis (CIE) was shown to be a very sensitive method for detecting precipitins, but not for their measurement. A prospective evaluation of immunodiffusion, latex agglutination and CIE as potential serodiagnostic techniques for farmer's lung disease is suggested.


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