Objectives:
Evaluate the effects of "XGTQ" in the treatment of cirrhosis induced by Carbon tetrachloride (CCL4)
in combination with alcohol and high-fat diet on Wistar rats.
Materials and methods:
Cirrhosis on white rats was induced by subcutaneously injecting CC14 at an initial dose of
5,0ml/kg, followed by 1,2ml/kg once a week in 10 weeks. Then, fed with synthetic food, added 20% fat, and 0.05% cholesterol and iron oxalate. Rats were administered every day with plain water and 1 day with water mixed with 30% ethanol.
The rats were randomly divided into 5 groups and given distilled water (group 1 and 2 or control group), silymarin (group
3 or reference group) or the "XGTQ" drug extract (group 4, 5) for 4 weeks. Collected blood for biochemical test and liver
were dissected to evaluate weight, morphology and quantified 4-hydroxyproline to evaluate fibrosis and collagen accumulation.
Results:
In cirrhotic wistar rats, "XGTQ" drug at 19.6 g/kg/24h and 58.8 g/kg/24h showed the ability of reducing the activity
of enzymes AST, ALT in the blood (p<0.01), increasing plasma albumin and decreasing prothrobin time (p<.05);
improving physical condition, macroscopic and microscopic images of H&E-stained liver; decreasing the concentration of
hydroxyproline in the liver and reducing the level of cirrhosis on the masson-stained templates. The effects of "XGTQ"
increased with the dose, and was equivalent to silymarin at the dose of 70 mg/kg/24h.
Conclusion:
The extract of "XGTQ" drug is effective in treating cirrhosis in Wistar rats.