THE KINETIC DPPH-METHOD OF ANTIRADICAL ACTIVITY ANALYSIS OF THE MATERIALS OF PLANT ORIGIN

2015 ◽  
pp. 425-442
Keyword(s):  
Antiradical Activity ◽  
Activity Analysis ◽  
Plant Origin ◽  
Dpph Method

Chemical composition of propolis from Canada, its antiradical activity and plant origin

2005 ◽  
Vol 19 (7) ◽  
pp. 673-678 ◽  
Author(s):  
Roumen Christov ◽  
Boryana Trusheva ◽  
Milena Popova ◽  
Vassya Bankova ◽  
Michel Bertrand
Keyword(s):  
Chemical Composition ◽  
Antiradical Activity ◽  
Plant Origin

scholarly journals Antioxidant Activities and Total Phenolics of Acacia Honey

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S245-S247 ◽  
Author(s):  
M. Krpan ◽  
K. Marković ◽  
G. Šarić ◽  
B. Skoko ◽  
M. Hruškar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Antiradical Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Optical Rotation ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Acacia Honey

The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey.

scholarly journals Improvement of the Oxidative Stability of Sesame Oil Using Spirulina as a Natural Antioxidant

2018 ◽  
Author(s):  
Shima Omidi ◽  
Hamid Sarhadi ◽  
Fatemeh Shahdadi
Keyword(s):  
Oxidative Stability ◽  
Antiradical Activity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Sesame Oil ◽  
Butylated Hydroxytoluene ◽  
Natural Antioxidant ◽  
Synthetic Antioxidants ◽  
Methanol Extracts ◽  
Dpph Method

Background: Nowadays, natural antioxidants are used to replace synthetic antioxidants for delaying or preventing the oxidation of edible oils. In this study, phenolic compounds and antioxidant properties of methanol extracts of Spirulina microalgae (Arthrospira platensis) were measured. Methods: Phenolic content was measured by Folin–Ciocalteau method and antioxidant activity was measured by the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. Also, spirulina microalgae as natural antioxidant, was added to sesame oil for improving its oxidative stability. The effects of methanol extract of these microalgae in four concentrations (0, 500, 1000 and 2000 ppm) and synthetic antioxidants of butylated hydroxyl anyzole (BHA) and butylated hydroxytoluene (BHT) in two levels (100 and 200 ppm) in retarding the sesame oil oxidation were examined. Results: The results showed that spirulina extract had 50.54 mg gallic acid/g samples total phenols and antiradical activity (84.38%). By increasing the concentration of the extracts, the oxidation process decreased. Methanol extract at 1000 ppm concentration had the highest antiradical activity than other concentrations and its antiradical activity was alike the synthesis antioxidant of BHA. By Increasing of storage period, peroxide values in 500 and 1000 ppm of spirulina extracts first increased then decreased and in other treatment increased. During storage time thiobarbituric acid index increased. However, this increase was higher in control treatment than others. Conclusion: According to the stated content, it can be said that methanol extracts of spirulina have appropriate antioxidant properties and spirulina can be used as a natural antioxidant to improve the oxidative stability of sesame oil.

scholarly journals The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

2020 ◽  
Vol 9 (1) ◽  
pp. 8-15
Author(s):  
Galuh Widiyarti ◽  
◽  
Andini Sundowo ◽  
Euis Filailla ◽  
Joddy Arya Laksmono ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Trace Amount ◽  
Extraction Process ◽  
Activity Analysis ◽  
Hydraulic Press ◽  
Screw Press ◽  
Ic50 Value ◽  
Dpph Method ◽  
Twin Screw ◽  
Water Contents

The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.

scholarly journals Evaluation of Antiradical Activity Grape Seeds and Olive (Olea europaea) Pits Extracts

2013 ◽  
Vol 6 (1) ◽  
pp. 185-190
Author(s):  
F. Tajalli
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Maximum Activity ◽  
Fruit Juices ◽  
Scavenging Activity ◽  
Grape Seeds ◽  
Antiradical Properties ◽  
Environmental Treatment ◽  
Dpph Method

The antiradical activity of grape seeds and olive pits extracts were investigated. The aim of this study is evaluating the radical scavenging activity of methanol extracts of olive pits and grape seeds and to recover a functional and antiradical compound from olive oil and fruit juices factories waste and also for environmental treatment. The antiradical properties of ripe and unripe olive pits (RIOP and URIOP) and grape seeds (IGS) that are respectively used in Iranian oil industries and fruit juices producers are examined. All seeds and pits extracts showed DPPH radical scavenging activity ranging from 24.51 to 97.06. For this purpose a methanolic extract was prepared from each of the RIOP, URIOP and IGS and their radical scavenging ability is determined with DPPH method. For this trial the effect of 3 different dilutions (100, 200 and 300 ?g/L) of RIOP, URIOP, IGS extracts was used, separately. It was appeared that in above 100 ppm concentrations the antiradical properties reaches to its maximum activity. Also, IGS extract shows better effects in 100 ppm concentrations in comparison with RIOP, URIOP and ascorbic acid. The study shows that grape seeds can be used as a rich source of functional and antiradical compound and anticancer drugs production. Keywords: Iranian olive pits; DPPH; Antiradical activity; Radical scavenging; Grape seeds.  © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.  doi: http://dx.doi.org/10.3329/jsr.v6i1.16654 J. Sci. Res. 6 (1), 185-190 (2014)

scholarly journals Morphological and antiradical characteristics of Rugosa rose (Rosa rugosa Thunb.) fruits canned in different kind of honeys and in beverages prepared from honey

10.5219/1065 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 497-506
Author(s):  
Katarína Fatrcová-Šramková ◽  
Ján Brindza ◽  
Eva Ivanišová ◽  
Tünde Juríková ◽  
Marianna Schwarzová ◽  
...  
Keyword(s):  
Methyl Alcohol ◽  
Antiradical Activity ◽  
Black Locust ◽  
Fresh Fruit ◽  
Fruit Pulp ◽  
Morphometric Traits ◽  
Rosa Rugosa ◽  
Alcohol Extract ◽  
Dpph Method ◽  
Fresh Pulp

The aim of the work was to determined the basic morphological and morphometric traits of rugosa rose (Rosa rugosa Thunb.) and antiradical activity of fruit pulp canned in different kind of honeys and in beverages prepared from honey. In experiments there were used 4 genotypes of roses originated from arboretum Mlyňany (Slovakia). The evaluation of 11 morphometric traits of fruit showed that the average weight of the fresh fruit without pedicle reached up 5.14 – 5.46 g, the weight of pedicle was 0.05 – 0.08 g, weight of pulp and seeds 4.80 – 5.13 g, weight of calyx 0.25 – 0.31 g, length and width of fruit (16.10 – 18.13 mm, 21.38 – 22.46 mm), the number of seeds in fruit 48.45 – 71.05, thickness of pulp 2.63 – 2.97 mm. Separated fruit pulp was canned at 40 °C and 80 °C and premixed in robinia honey and honeydew honey. Beverages were prepared by mixture of fruit pulp in honey (15 g) with cold water (150 mL). Antiradical activity was determined by DPPH method in fruit pulp (in methyl alcohol and water extracts), in honeys (black locust honey and honeydew honey) and beverages. There had been confirmed statistically significant differences in morphological traits, especially in colour and shape of fruit. Antiradical activity of fresh fruit pulp in methyl alcohol extract was determined 94.59%, in water extract 89.71%. Antiradical activity of black locust honey was 7.63%, honeydew honey 6.54%. Antiradical activity was determined also adding honeydew honey and black locust honey to fresh pulp of fruit prepared at 80 °C (33.55% and 77.58%). In beverages prepared from fresh pulp, honey and water it was investigated the higher values of antiradical activity in samples with addition of honeydew honey (81.81 – 83.86%) in comparison with robinia honey (75.57 – 79.96%).

scholarly journals Determination of antiradical activity and phenolic and flavonoid contents of extracts and fractions of jackfruit (Artocarpus heterophyllus Lamk) seeds

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 36-43
Author(s):  
Zubaydah ◽  
M.H. Sahumena ◽  
W.O.N. Fatimah ◽  
Sabarudin ◽  
M. Arba ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antiradical Activity ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Artocarpus Heterophyllus ◽  
Water Fraction ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Jackfruit (Artocarpus heterophyllus Lamk) is a plant from the Moraceae family that is widespread in Indonesia. Empirically, jackfruit can be used to cure hypertension, diabetes, cancer, asthma, dermatosis, coughs, wounds, acne, and diarrhea. The bioactive compounds in jackfruit include phenolics and flavonoids, which function as natural antioxidants. This research investigated the antiradical activity of jackfruit seed extracts and fractions using DPPH (2-2-diphenyl-1-picrylhydrazyl) and examined the total phenolic and flavonoid contents which may be developed as functional medicines and foods. The jackfruit seed powder was extracted using the maceration method. Radical scavenging activities were measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic contents were determined using the Folin-Ciocalteu method. Meanwhile, the contents of flavonoids were determined using the aluminum chloride complex colorimetric method. The IC50 value of ethyl acetate fraction, methanol extract, n -hexane fraction, and water fraction was 5.435±0.064 µg/mL, 5.639±0.302 µg/mL, 7.201±0.475 µg/mL, and 9.134±0.2911 µg/mL, respectively. The phenolic and flavonoid contents of ethyl acetate fraction, methanol extract, n-hexane fraction, and water fraction were 49.597±1.589, 47.949±1.966, 41.214±4.354 and 35.504±0.913 g GAE/100 g of sample, respectively for phenolic, and 70.199±0.458, 65.228±0.615, 59.907±0.719, and 54.234±0.351 g of quercetin equivalent/100 g of sample, respectively of flavonoids, with a correlation value (R2 ) to antiradical power of 0.4582 for phenolic and 0.5281 for flavonoids. The ethyl acetate fraction of jackfruit seeds can be further developed as an anti -radicals and functional food.

STUDY OF THE ANTIOXIDANT PROPERTIES OF PLANT EXTRACTS USING THE DPPH METHOD

2021 ◽  
Vol 22 (4) ◽  
pp. 22-30
Author(s):  
A.N. Nikiforova ◽  
◽  
A.V. Samoilov ◽  
Yu.V. Nikolaeva ◽  
E.M. Fedorova ◽  
...  
Keyword(s):  
Binding Capacity ◽  
Food Additives ◽  
Antioxidant Properties ◽  
Food Products ◽  
Scientific Work ◽  
Grape Seed ◽  
Plant Origin ◽  
Dpph Method ◽  
Series Of Experiments ◽  
Active Substances

In modern food products, food additives of natural origin are increasingly used. The search for ef-fective antioxidants of plant origin is a widely studied topic at the present time. The study of this issue makes it possible to introduce the latest solutions in the fight against lipid oxidation in food products. The aim of this scientific work was to evaluate the radical-binding capacity of plant ex-tracts with different contents of active substances. As a result of a series of experiments using the DPPH method, the antioxidant properties of 14 samples were investigated. It was found that the greatest antioxidant activity among the tested extracts is possessed by extracts of green tea, apple, grape seed, sorghum. The rest of the samples are moderately active.

scholarly journals Comparative analysis of the chemical composition and antioxidant properties of different types of tea as a raw material for the production of tea extracts

2018 ◽  
Vol 80 (2) ◽  
pp. 249-255 ◽  
Author(s):  
D. F. Valiulina ◽  
N. V. Makarova ◽  
D. V. Budylin
Keyword(s):  
Green Tea ◽  
Antiradical Activity ◽  
Antioxidant Properties ◽  
Total Content ◽  
Natural Antioxidants ◽  
Raw Material ◽  
Soluble Solids ◽  
Restoring Force ◽  
Dpph Method ◽  
Phenolic Substances

One of the most frequently consumed sources of natural antioxidants is tea. High content of phenolic substances in it, makes it an excellent functional drink. At present, interest in tea is great not only as an independent drink, but also as a raw material for the production of tea extracts. The study was conducted on extracts obtained from samples of leaf tea of foreign and Russian origin in the following parameters: the content of soluble solids in tea extracts; total content of phenolic substances; total content of flavonoids; total content of tannins; antiradical activity by the DPPH method; restoring force by the method of FRAP. During the study of tea species, different in the way of processing and in the region of origin, it was determined that the leader among the presented teas is the white tea of Chinese origin, which has the highest antiradical activity and the highest content of tannins. Comparing green tea from China and Russia, it can be concluded that the content of individual groups of substances varies slightly against the background of slightly higher anti-radical activity of Chinese tea. In the group of black teas, the Indian "Harmutti" tea is in the lead by the results of the test, significantly exceeding tea from the Krasnodar Territory by anti-radical activity and the total content of phenolic substances. Thus, promising types of tea for the prevention of diseases (diabetes, atherosclerosis, hypertension, Alzheimer's disease), arising as a consequence of oxidative stress, are tea white Chinese and green tea Krasnodar.

Sign in / Sign up

Export Citation Format

Share Document