Enhancing Separation Efficiency: On-Chip Multidimensional Separation and Determination of Urinary Proteins by MC-CE Device

2015 ◽  
pp. 132-147
Keyword(s):  
Separation Efficiency ◽  
Urinary Proteins ◽  
Multidimensional Separation ◽  
On Chip

scholarly journals A Comparative Study of Advanced Stationary Phases for Fast Liquid Chromatography Separation of Synthetic Food Colorants

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3335
Author(s):  
Ivona Lhotská ◽  
Petr Solich ◽  
Dalibor Šatínský
Keyword(s):  
Separation Efficiency ◽  
Water Solution ◽  
Stationary Phases ◽  
Gradient Elution ◽  
Synthetic Food Colorants ◽  
Liquid Chromatography Separation ◽  
Method Optimization ◽  
Superficially Porous Particles ◽  
Fast Liquid Chromatography

Food analysis demands fast methods for routine control and high throughput of samples. Chromatographic separation enables simultaneous determination of numerous compounds in complex matrices, several approaches increasing separation efficiency and speed of analysis were involved. In this work, modern types of column with monolithic rod or superficially porous particles were employed and compared for determination of eight synthetic food dyes, their chromatographic performance was evaluated. During method optimization, cyano stationary phase Chromolith Performance CN 100 × 4.6 mm and Ascentis Express ES-CN 100 × 4.6 mm, 5 µm were selected for the separation of polar colorants. The separation was performed by gradient elution of acetonitrile/methanol and 2% water solution of ammonium acetate at flow rate 2.0 mL min−1. Mobile phase composition and the gradients were optimized in order to enable efficient separation on both columns. The method using fused-core particle column provided higher separation efficiency, narrow peaks of analytes resulted in increased peak capacity and shortening of analysis time. After the validation, the method was applied for analysis of coloured beers, soft drinks and candies.

Determination of Nitrate in Water by HPLC

2013 ◽  
Vol 448-453 ◽  
pp. 406-408
Author(s):  
Jing Liu ◽  
Xiao Na Ji ◽  
Qing Kai Ren ◽  
Sheng Shu Ai ◽  
Li Jun Wan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Recovery Rate ◽  
High Performance ◽  
Separation Efficiency ◽  
Fast Analysis ◽  
Relative Standard

We established a method fordetermination of nitrate in water by High Performance Liquid Chromatography(HPLC). The sample was analysed by HPLC-ADA and was quantitated by externalstandard method after being simply processed. This methd has the advantages ofhigh separation efficiency and fast analysis. The experiment result showed thatthe linearly dependent coefficient was0.994, the recovery rate was between 98.7%～105.7%,the relative standard deviation（RSD）wasless than 2.1 %, and the lowest detectable limit is 0.01ng (S/N=1.6).

High-resolution capillary electrophoresis for the determination of carbamylated albumin

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Julien Favresse ◽  
Joris Delanghe
Keyword(s):  
Separation Efficiency ◽  
Theoretical Plate ◽  
Clinical Routine ◽  
Negative Prognostic Factor ◽  
Symmetry Factor ◽  
Primary Amino ◽  
The Mean ◽  
Dose Dependent

Abstract Objectives Carbamylation is a non-enzymatic post-translational reaction of a primary amino group of a protein with isocyanate. The albumin carbamylation is a negative prognostic factor in chronic kidney disease (CKD) patients and induce charge difference implying an observed shift in electrophoretic mobility that can be measured through a symmetry factor (SF). Methods The Helena V8 and the Sebia Capillarys 2 systems were used for all experiments. The effect of in vitro carbamylation on the SF by spiking increasing concentrations of potassium isocyanate (KCNO) in serum of three healthy volunteers was investigated. Theoretical plate numbers (N) as a surrogate of separation efficiency were also calculated and correlations between SF and renal function biomarkers were performed on 284 patients. Results A dose-dependent impact of KCNO on the SF was observed for both methods with the Helena V8 being more sensitive. The mean N was significantly higher on the Helena V8 as compared to the Sebia Capillarys 2 (2,972 vs. 444.1, p<0.0001). The SF correlated significantly with eGFR (r=0.50, p<0.0001), creatinine (r=−0.31, p<0.0001) and urea (r=−0.34, p<0.0001) on the Helena V8. On the Sebia Capillarys 2, a significant correlation was only observed with eGFR (r=0.17, p=0.004). A better discrimination between CKD stages was also observed using the Helena V8. Conclusions Thanks to a higher mean N, the Helena V8 might offer new possibilities, including detection of carbamylated albumin through SF calculation. Further studies are still needed to confirm the interest of using this type of assays in clinical routine.

Automated Immunochemical Method for Determination of Urinary Protein of Plasma Origin

1975 ◽  
Vol 21 (10) ◽  
pp. 1465-1468 ◽  
Author(s):  
Lawrence M Killingsworth ◽  
Carol E Britain ◽  
Linda L Woodard
Keyword(s):  
Polyethylene Glycol ◽  
Reaction Time ◽  
Human Plasma ◽  
Continuous Flow ◽  
Rapid Determination ◽  
Reaction Medium ◽  
Urinary Proteins ◽  
Immunochemical Method ◽  
Antigen Antibody

Abstract An automated continuous-flow procedure has been developed for the rapid determination of urinary proteins of plasma origin. Antiserum to whole human plasma was used as the reagent, and the antigen—antibody reactions were quantitated by nephelometry. By adding polyethylene glycol (mol wt 6000-7500) to the reaction medium, reaction time was decreased to &lt;3 min; no sample blanks were required; and samples were analyzed at a rate of 70 per hour. Recovery studies yielded an average of 98.5% of the added protein. In-run replicate precision (CV) of the method was 1.45%; day-to-day precision was 2.58%.

scholarly journals Solid‐state ion sensor for on‐chip determination of potassium ion in body fluid

2020 ◽  
Vol 3 (6) ◽  
Author(s):  
Prem C. Pandey ◽  
Govind Pandey ◽  
Roger J. Narayan
Keyword(s):  
Solid State ◽  
Body Fluid ◽  
Potassium Ion ◽  
Ion Sensor ◽  
On Chip

Determination of Nitrophenol Isomers in Water

2015 ◽  
Vol 1083 ◽  
pp. 80-83
Author(s):  
Xiao Na Ji ◽  
Sheng Shu Ai ◽  
Juan Tang ◽  
Hong Qu ◽  
De Jun Bian
Keyword(s):  
Separation Efficiency ◽  
Tap Water ◽  
Target Object ◽  
Hplc Method ◽  
The Other ◽  
Municipal Sewage ◽  
External Standard Method ◽  
External Standard ◽  
High Separation

This paper establishes the HPLC method of determining three nitrophenol isomers in municipal sewage, surface water and tap water. After the sample is filtered and distilled, conduct the monitoring analysis on the distillate with HPLC-DAD and quantify it in external standard method. In the municipal sewage treated in bioanalysis method one type of target object o-nitrophenol is found, whose content is 0.11mg·L-1, no target object is found in all the other samples. This method has the adventages of high separation efficiency and high analysis rate. The experiment result shows that the linear correlation coefficient is above 0.983, the recovery rate lies between 78.6% and 105.2%, the minimum detection limit is 0.05mg·L-1(S/N=3.0).

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