Surface Characteristics Dictate Microbial Adhesion Ability

2015 ◽  
pp. 212-233 ◽  
Keyword(s):  
Surface Characteristics ◽  
Microbial Adhesion ◽  
Adhesion Ability

The impact of growth history and flagellation on the adhesion of variousListeria monocytogenesstrains to polystyrene

2009 ◽  
Vol 55 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Odile Tresse ◽  
Véronique Lebret ◽  
Dominique Garmyn ◽  
Olivier Dussurget
Keyword(s):  
Food Industry ◽  
Surface Characteristics ◽  
Bacterial Surface ◽  
Ph Values ◽  
Adhesion Ability ◽  
Growth History ◽  
Encoding Gene ◽  
Inert Surfaces ◽  
The Impact ◽  
Adhesion Process

The contribution of growth history and flagella to adhesion of Listeria monocytogenes was analysed. An in-frame deletion on the flagellin encoding gene (flaA) was performed in L. monocytogenes EGD-e to compare its adhesion ability with the parental strain, after cultivation at various pH values and temperatures. The pH, as well as the temperature, affected the adhesion of L. monocytogenes EGD-e. In addition, the adhesion of L. monocytogenes EGD-e was reduced in energy-depressed cells. Conversely, the physicochemical bacterial surface characteristics affected by growth history did not influence the adhesion. Adhesion variations observed among environmental and clinical strains was attributed to the flagella. The naturally aflagellated strains resulted in an adhesion capacity similar to that observed for mutants and parental strains cultivated under flagellum expression repressing conditions. However, L. monocytogenes is able to adhere to inert surfaces through a residual adhesion process without flagella. All these observations emphasize the importance to consider the food environmental factors in the risk assessment of L. monocytogenes in food industry.

scholarly journals Effect of Surface Characteristics of Different Implant Abutment Materials on the Microbial Adhesion- An Invitro Study

2021 ◽  
Author(s):  
Vimal Munot ◽  
Ramesh P Nayakar ◽  
Raghunath Patil
Keyword(s):  
Surface Roughness ◽  
Stainless Steel ◽  
Surface Energy ◽  
Test Group ◽  
Surface Characteristics ◽  
Microbial Adhesion ◽  
Implant Abutment ◽  
Group B ◽  
Pair Wise Comparison ◽  
Effect Of Surface

Introduction: In two-stage implants, micro-gap between the fixture and the abutment and the superstructure are the potential areas to cause peri-implantitis. The surface roughness and surface energy of the implant abutment materials play an important role in the microbial colonisation on their surface and can help the clinician to choose a better implant abutment material in terms of microbial affinity. Aim: To evaluate the effect of surface characteristics of different implant abutment materials on microbial adhesion. Materials and Methods: The comprehensive analytical experimental study was conducted at KAHER KLE VK Institute of Dental Sciences, Belagavi between Jan 2017- Dec 2017. Forty-five (n=45) identical disc shaped specimens were fabricated using; Zirconia (Group A), Titanium alloy (Group B) and Surgical Grade Stainless Steel (Group C). The surface roughness was assessed for all test groups by Profilometer and Scanning Electron Microscope (SEM). The Surface energy was evaluated for all the test group specimens using Goniometer. Microbial adhesion and assessment were performed using sonicating and vortexing method for all the three groups using three different bacterial strains. The Colony Forming Units for all the specimens were tabulated and subjected to statistical analysis to draw the conclusions from the resultant data.The resultant data was analysed using SPSS software (Version 20). In order to collectively compare the means of the study groups pair-wise comparison of the test group was done using paired t-test with (p<0.05), and correlation between the surface parameters and CFU counts was done using Karl Pearson’s correlation coefficient. Results: On pair-wise comparison of three Groups (A, B, and C) with respect to surface roughness, there were statistically significant differences in surface roughness Ra values between all the groups p<0.001. On pair-wise comparison of all the three Groups with respect to surface energy, there were statistically significant differences in Wetting Angle (WA) values between all the groups (p<0.001**) except between group B and A (p=0.15). Zirconia showed the least CFU counts for Pi and Aa though the differences were not statistically significant. Karl Pearson’s correlation coefficient between surface roughness and surface energy with CFU counts showed a strongly positive correlation for all microbial species and were statistically significant p<0.001**. Conclusion: There was a strongly positive correlation of surface roughness and surface energy to CFU counts. Zirconia showed a low colonisation potential against P.intermedia and A.actinomycetemcomitans than titanium alloy and surgical grade stainless steel.

Adhesion of Dairy Propionibacteria to Intestinal Epithelial Tissue In Vitro and In Vivo

2002 ◽  
Vol 65 (3) ◽  
pp. 534-539 ◽  
Author(s):  
GABRIELA ZÁRATE ◽  
VILMA I. MORATA de AMBROSINI ◽  
ADRIANA PEREZ CHAIA ◽  
SILVIA N. GONZÁLEZ
Keyword(s):  
Surface Characteristics ◽  
Epithelial Tissue ◽  
Intestinal Epithelial ◽  
Adhesive Properties ◽  
Bacterial Surface ◽  
Beneficial Effects ◽  
Adhesion Ability ◽  
Dairy Propionibacteria

Adhesion to the intestinal mucosa is a desirable property for probiotic microorganisms and has been related to many of their health benefits. In the present study, 24 dairy Propionibacterium strains were assessed with regard to their hydrophobic characteristics and their autoaggregation and hemagglutination abilities, since these traits have been shown to be indicative of adherence in other microorganisms. Six strains were further tested for their capacity to adhere to ileal epithelial cells in vitro and in vivo. The results of the study showed that propionibacteria were highly hydrophilic, and hemagglutination and autoaggregation were properties not commonly found among these microorganisms. No relationship was found between surface characteristics and adhesion ability, since hemagglutinating, autoaggregating, and nonautoaggregating bacteria were able to adhere to intestinal cells both in vitro and in vivo. Microscopic examination revealed that autoaggregating cells adhered in clusters, with adhesion being mediated by only a few bacteria, whereas the hemagglutinating and nonautoaggregating strains adhered individually or in small groups making contact with each epithelial cell with the entire bacterial surface. The in vitro assessment of adhesion was a good indication of the in vivo association of propionibacteria with the intestinal epithelium. Therefore, the in vitro method presented here should be valuable in screening routinely adhesive properties of propionibacteria for probiotic purposes. The adhesion ability of dairy propionibacteria would prolong their maintenance in the gut and increase the duration of their provision of beneficial effects in the host, supporting the potential of Propionibacterium in the development of new probiotic products.

The Role of Biofilms and Material Surface Characteristics in Microbial Adhesion to Maxillary Obturator Materials: A Literature Review

2019 ◽  
Vol 57 (4) ◽  
pp. 487-498
Author(s):  
Karl M. Lyons ◽  
Richard D. Cannon ◽  
John Beumer ◽  
Mahmoud M. Bakr ◽  
Robert M. Love
Keyword(s):  
Literature Review ◽  
Surface Characteristics ◽  
Material Surface ◽  
Microbial Adhesion ◽  
Microbial Biofilms ◽  
Characteristic Features ◽  
Biofilm Development ◽  
And Function ◽  
Maxillary Defects

Background: Maxillofacial prosthetics includes restoration of maxillary defects resulting from resection of palate and nasosinus neoplasms with obturator prostheses which may be colonized by microorganisms and function as a reservoir of infection. Patients with neoplasms commonly also require radiotherapy that can result in changes in saliva quality and quantity and changes in the oral microbial flora. The altered flora, in individuals immunocompromised from cancer therapy, increases their risk of prosthesis-related infections. Objectives: In this review article, we explore microbial biofilms, their main components, mechanisms of microbial adhesion, and stages of biofilm development. We also discuss the different materials that are used for manufacturing maxillary obturators, their characteristic features, and how these can affect microbial adhesion. Furthermore, we shed some light on the factors that affect microbial adhesion to the surface of maxillary obturators including tissue proteins, protein adsorption, and the acquired enamel pellicle. Conclusions: The conclusions drawn from this literature review are that it is imperative to minimize the risk of local and systemic infections in immunocompromised patients with cancer having maxillary defects. It is also important to determine the role of saliva in microbial adhesion to obturator materials as well as develop materials that have a longer life span with surface characteristics that promote less microbial adhesion than current materials.

Medial Smooth Muscle Cell Proliferation in the Balloon Injured Rabbit Aorta: Effect of a Thiazole Compound with Platelet Inhibitory Activity

1984 ◽  
Vol 51 (01) ◽  
pp. 075-078 ◽  
Author(s):  
R G Schaub ◽  
C A Simmons
Keyword(s):  
Smooth Muscle ◽  
Platelet Aggregation ◽  
Balloon Catheter ◽  
Rabbit Aorta ◽  
Surface Characteristics ◽  
Inhibitory Effect ◽  
Lesion Size ◽  
Blue Staining ◽  
Morphologic Characteristics ◽  
Time Period

SummaryTwenty-seven adult male New Zealand rabbits (3–4 kgs) were used in this study. Six rabbits received vehicle, 3 groups of 6 each received doses of 4,5-bis(p-methoxyphenyl)-2-(trifluoromethyl)- thiazole, (U-53,059), at 0.3 mg/kg, 3.0 mg/kg and 30.0 mg/kg/day respectively. Drug and vehicle doses were given orally each day starting 3 days before balloon injury and continuing for the entire 2 week time period. Three rabbits were used as nontreated sham controls. In the vehicle and U-53,059 treated groups aortae were denuded of endothelial cells by balloon catheter injury. Two weeks after injury platelet aggregation to collagen was measured and the aortae removed for analysis of surface characteristics by scanning electron microscopy and lesion size by morphometry. All doses of U-53,059 inhibited platelet aggregation. The 3.0 and 30.0 mg/kg groups had the greatest inhibitory effect. All balloon injured aortae had the same morphologic characteristics. All vessels had similar extent and intensity of Evan’s blue staining, similar areas of leukocyte/platelet adhesion, and a myointimal cell cover of transformed smooth muscle cells. The myointimal proliferative response was not inhibited at any of the drug doses studied.

Examination of the lubricant storing and releasing ability of thermally sprayed surfaces

2011 ◽  
Vol 2 (2) ◽  
pp. 101-105
Author(s):  
L. Fazekas ◽  
Z. S. Tiba ◽  
G. Kalácska
Keyword(s):  
Free Energy ◽  
Surface Energy ◽  
Surface Free Energy ◽  
Essential Role ◽  
Index Number ◽  
Adhesion Ability ◽  
Thermally Sprayed ◽  
Proper Operation

Abstract The lubricant storing and releasing ability of the thermally sprayed surfaces plays an essential role in the proper operation of the components. In the case of porous sprayed surfaces the lubricant storing and releasing ability depends mainly on porosity and the surface energy (adhesion susceptibility). The adhesion ability can also be expressed indirectly with an index number that is by determining the surface free energy.

Photocatalytic activity of TiO2 synthesized by anodization and anodic spark deposition

MRS Advances ◽  
2020 ◽  
Vol 5 (61) ◽  
pp. 3141-3152
Author(s):  
Alma C. Chávez-Mejía ◽  
Génesis Villegas-Suárez ◽  
Paloma I. Zaragoza-Sánchez ◽  
Rafael Magaña-López ◽  
Julio C. Morales-Mejía ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Photocatalytic Activity ◽  
Surface Characteristics ◽  
Amorphous Solids ◽  
X Ray Diffraction ◽  
X Ray ◽  
Catalytic Activities ◽  
Anodic Spark Oxidation ◽  
Porous Surfaces ◽  
Scanning Electron

AbstractSeveral photocatalysts, based on titanium dioxide, were synthesized by spark anodization techniques and anodic spark oxidation. Photocatalytic activity was determined by methylene blue oxidation and the catalytic activities of the catalysts were evaluated after 70 hours of reaction. Scanning Electron Microscopy and X Ray Diffraction analysis were used to characterize the catalysts. The photocatalyst prepared with a solution of sulfuric acid and 100 V presented the best performance in terms of oxidation of the dye (62%). The electric potential during the synthesis (10 V, low potential; 100 V, high potential) affected the surface characteristics: under low potential, catalyst presented smooth and homogeneous surfaces with spots (high TiO2 concentration) of amorphous solids; under low potential, catalyst presented porous surfaces with crystalline solids homogeneously distributed.

Soft Tissue Responses and Attachment to Porous Titanium with Different Surface Characteristics

2006 ◽  
Vol 9 (2) ◽  
pp. 127-134
Author(s):  
Yao Wu ◽  
Hu Li ◽  
Tun Yuan ◽  
Chunlin Deng ◽  
Bangcheng Yang ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Surface Characteristics ◽  
Porous Titanium ◽  
Tissue Responses

Rubber–Road Contact: Comparison of Physics-Based Theory and Indoor Experiments

2016 ◽  
Vol 44 (3) ◽  
pp. 150-173 ◽  
Author(s):  
Mehran Motamedi ◽  
Saied Taheri ◽  
Corina Sandu
Keyword(s):  
Rough Surface ◽  
Practical Importance ◽  
Surface Profile ◽  
Surface Characteristics ◽  
Mechanical Analysis ◽  
Friction Model ◽  
Rubber Friction ◽  
Optical Profilometer ◽  
Viscoelastic Modulus ◽  
Longitudinal Slip

ABSTRACT For tire designers, rubber friction is a topic of pronounced practical importance. Thus, development of a rubber–road contact model is of great interest. In this research, to predict the effectiveness of the tread compound in a tire as it interacts with the pavement, the physics-based multiscale rubber-friction theories developed by B. Persson and M. Klüppel were studied. The strengths of each method were identified and incorporated into a consolidated model that is more comprehensive and proficient than any single, existing, physics-based approach. In the present work, the friction coefficient was estimated for a summer tire tread compound sliding on sandpaper. The inputs to the model were the fractal properties of the rough surface and the dynamic viscoelastic modulus of rubber. The sandpaper-surface profile was measured accurately using an optical profilometer. Two-dimensional parameterization was performed using one-dimensional profile measurements. The tire tread compound was characterized via dynamic mechanical analysis. To validate the friction model, a laboratory-based, rubber-friction test that could measure the friction between a rubber sample and any arbitrary rough surface was designed and built. The apparatus consisted of a turntable, which can have the surface characteristics of choice, and a rubber wheel in contact with the turntable. The wheel speed, as well as the turntable speed, could be controlled precisely to generate the arbitrary values of longitudinal slip at which the dynamic coefficient of friction was measured. The correlation between the simulation and the experimental results was investigated.

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