Heterogeneity of the CNS Microvascular Endothelium

2015 ◽  
pp. 70-85
Author(s):  
Joel Pachter ◽  
Debayon Paul ◽  
Shujun Ge
Keyword(s):  
Microvascular Endothelium

A Porcine Model for Adipose Tissue-Derived Endothelial Cell Transplantation

1992 ◽  
Vol 1 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Carlton Young ◽  
Bruce E. Jarrell ◽  
James B. Hoying ◽  
Stuart K. Williams
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Endothelial Cell ◽  
Cell Transplantation ◽  
Animal Studies ◽  
Porcine Model ◽  
Cell Isolation ◽  
Microvascular Endothelial Cell ◽  
Microvascular Endothelium ◽  
Microvascular Endothelial

The transplantation of endothelial cells represents a technology which has been suggested for applications ranging from improvement in function of implanted vascular devices to genetic therapy. The use of microvascular endothelial cell transplantation has seen increased use both in animal studies as well as clinical use. This report describes our techniques for the isolation and establishment of initial cultures of microvascular endothelial cells derived from porcine fat. A variety of anatomic sites within the pig were evaluated to determine the appropriateness of different sources of fat for endothelial cell isolation. The properitoneal fat was determined to be optimal due to the predominance of endothelium in this tissue and the ease of isolation of microvascular endothelium following collagenase digestion. The study of endothelial cell transplantation in the porcine model is now possible using the methods described for adipose tissue-derived micro vessel endothelial cell isolation.

Malaria pigment stimulates chemokine production by human microvascular endothelium

Acta Tropica ◽  
2017 ◽  
Vol 172 ◽  
pp. 125-131 ◽  
Author(s):  
Nicoletta Basilico ◽  
Yolanda Corbett ◽  
Sarah D' Alessandro ◽  
Silvia Parapini ◽  
Mauro Prato ◽  
...  
Keyword(s):  
Microvascular Endothelium ◽  
Chemokine Production ◽  
Malaria Pigment

scholarly journals Signalling between microvascular endothelium and cardiomyocytes through neuregulin

2014 ◽  
Vol 102 (2) ◽  
pp. 194-204 ◽  
Author(s):  
Emily M. Parodi ◽  
Bernhard Kuhn
Keyword(s):  
Microvascular Endothelium

Endothelin-1 production by a microvascular endothelial cell line treated with Plasmodium falciparum parasitized red blood cells

2002 ◽  
Vol 103 (s2002) ◽  
pp. 464S-466S ◽  
Author(s):  
Nicoletta BASILICO ◽  
Livianna SPECIALE ◽  
Silvia PARAPINI ◽  
Pasquale FERRANTE ◽  
Donatella TARAMELLI
Keyword(s):  
Plasmodium Falciparum ◽  
Endothelial Cell ◽  
Red Blood Cells ◽  
Cell Line ◽  
Blood Cells ◽  
Microvascular Endothelial Cell ◽  
Microvascular Endothelium ◽  
Endothelial Cell Line ◽  
Endothelin 1 ◽  
Microvascular Endothelial

In this study, we investigated the production of endothelin 1 (ET-1) by a human microvascular endothelial cell line, HMEC-1, co-cultured with Plasmodium falciparum-parasitized red blood cells (pRBCs). The results indicate that hypoxia increased the basal level of ET-1 production by HMEC-1 cells after 24 or 48h of treatment. However, the co-incubation of HMEC-1 cells with pRBCs, but not with uninfected RBCs, induced a dose-dependent decrease of both constitutive and hypoxia-induced ET-1 production. The inhibition was not due to a decrease in cell viability, as lactate dehydrogenase release remained constant. These results indicate that pRBCs are able to interfere with both the constitutive and stimulated ET-1 release from the microvascular endothelium, thus inducing local modifications of the vascular tone and of the inflammatory response. This could be of relevance in the pathogenesis of the most severe forms of P. falciparum infections, such as cerebral malaria or malaria during pregnancy.

scholarly journals Blood—Brain Barrier Genomics

2001 ◽  
Vol 21 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Jian Yi Li ◽  
Ruben J. Boado ◽  
William M. Pardridge
Keyword(s):  
Gene Expression ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Specific Gene ◽  
Microvascular Endothelium ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Specific Gene Expression ◽  
Blood Brain ◽  
Tester Cdna

The blood–brain barrier (BBB) is formed by the brain microvascular endothelium, and the unique transport properties of the BBB are derived from tissue-specific gene expression within this cell. The current studies developed a gene microarray approach specific for the BBB by purifying the initial mRNA from isolated rat brain capillaries to generate tester cDNA. A polymerase chain reaction–based subtraction cloning method, suppression subtractive hybridization (SSH), was used, and the BBB cDNA was subtracted with driver cDNA produced from mRNA isolated from rat liver and kidney. Screening 5% of the subtracted tester cDNA resulted in identification of 50 gene products and more than 80% of those were selectively expressed at the BBB; these included novel gene sequences not found in existing databases, ESTs, and known genes that were not known to be selectively expressed at the BBB. Genes in the latter category include tissue plasminogen activator, insulin-like growth factor-2, PC-3 gene product, myelin basic protein, regulator of G protein signaling 5, utrophin, IκB, connexin-45, the class I major histocompatibility complex, the rat homologue of the transcription factors hbrm or EZH1, and organic anion transporting polypeptide type 2. Knowledge of tissue-specific gene expression at the BBB could lead to new targets for brain drug delivery and could elucidate mechanisms of brain pathology at the microvascular level.

Influenza-Induced Priming and Leak of Human Lung Microvascular Endothelium upon Exposure toStaphylococcus aureus

2015 ◽  
Vol 53 (4) ◽  
pp. 459-470 ◽  
Author(s):  
Changsen Wang ◽  
Susan M. Armstrong ◽  
Michael G. Sugiyama ◽  
Arata Tabuchi ◽  
Adrienn Krauszman ◽  
...  
Keyword(s):  
Human Lung ◽  
Microvascular Endothelium
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