Metataxonomic Profi ling and Prediction of Functional Behavior of Wheat Straw Degrading Microbial Consortia

2015 ◽  
pp. 141-172
Keyword(s):  
Wheat Straw ◽  
Microbial Consortia ◽  
Functional Behavior

scholarly journals Unveiling the metabolic potential of two soil-derived microbial consortia selected on wheat straw

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Diego Javier Jiménez ◽  
Diego Chaves-Moreno ◽  
Jan Dirk van Elsas
Keyword(s):  
Wheat Straw ◽  
Microbial Consortia ◽  
Metabolic Potential

scholarly journals Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia

2014 ◽  
Vol 7 (1) ◽  
pp. 92 ◽  
Author(s):  
Diego Jiménez ◽  
Francisco Dini-Andreote ◽  
Jan van Elsas
Keyword(s):  
Wheat Straw ◽  
Microbial Consortia

Microscopic analysis of wheat straw cell wall degradation by microbial consortia for hydrogen production

2015 ◽  
Vol 40 (1) ◽  
pp. 151-160 ◽  
Author(s):  
Marisol Pérez-Rangel ◽  
Francisco R. Quiroz-Figueroa ◽  
Jaquelina González-Castañeda ◽  
Idania Valdez-Vazquez
Keyword(s):  
Cell Wall ◽  
Hydrogen Production ◽  
Wheat Straw ◽  
Microscopic Analysis ◽  
Microbial Consortia ◽  
Cell Wall Degradation

Hydrogen and butanol production from native wheat straw by synthetic microbial consortia integrated by species of Enterococcus and Clostridium

Fuel ◽  
2015 ◽  
Vol 159 ◽  
pp. 214-222 ◽  
Author(s):  
Idania Valdez-Vazquez ◽  
Marisol Pérez-Rangel ◽  
Adán Tapia ◽  
Germán Buitrón ◽  
Carlos Molina ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Microbial Consortia ◽  
Butanol Production ◽  
Synthetic Microbial Consortia

scholarly journals Effect of culture conditions on the performance of lignocellulose-degrading synthetic microbial consortia

2021 ◽  
Author(s):  
Yanfang Wang ◽  
Theo Elzenga ◽  
Jan Dirk van Elsas
Keyword(s):  
Wheat Straw ◽  
Fungal Growth ◽  
Growth Dynamics ◽  
Degradation Process ◽  
Culture Conditions ◽  
Microbial Consortia ◽  
Special Focus ◽  
Effects Of Ph ◽  
Cell Densities ◽  
L 62

AbstractIn this study, we examined a synthetic microbial consortium, composed of two selected bacteria, i.e., Citrobacter freundii so4 and Sphingobacterium multivorum w15, next to the fungus Coniochaeta sp. 2T2.1, with respect to their fate and roles in the degradation of wheat straw (WS). A special focus was placed on the effects of pH (7.2, 6.2, or 5.2), temperature (25 versus 28 °C), and shaking speed (60 versus 180 rpm). Coniochaeta sp. 2T2.1 consistently had a key role in the degradation process, with the two bacteria having additional roles. Whereas temperature exerted only minor effects on the degradation, pH and shaking speed were key determinants of both organismal growth and WS degradation levels. In detail, the three-partner degrader consortium showed significantly higher WS degradation values at pH 6.2 and 5.2 than at pH 7.2. Moreover, the two bacteria revealed up to tenfold enhanced final cell densities (ranging from log8.0 to log9.0 colony forming unit (CFU)/mL) in the presence of Coniochaeta sp. 2T2.1 than when growing alone or in a bacterial bi-culture, regardless of pH range or shaking speed. Conversely, at 180 rpm, fungal growth was clearly suppressed by the presence of the bacteria at pH 5.2 and pH 6.2, but not at pH 7.2. In contrast, at 60 rpm, the presence of the bacteria fostered fungal growth. In these latter cultures, oxygen levels were significantly lowered as compared to the maximal levels found at 180 rpm (about 5.67 mg/L, ~ 62% of saturation). Conspicuous effects on biomass appearance pointed to a fungal biofilm–modulating role of the bacteria.Key points• Coniochaeta sp. 2T2.1 has a key role in wheat straw (WS) degradation.• Bacterial impact shifts when conditions change.• pH and shaking speed are key drivers of the growth dynamics and WS degradation.

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

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