Characterization of microbial community in heavy oil Dongxin oilfield

2015 ◽  
pp. 47-50
Keyword(s):  
Microbial Community ◽  
Heavy Oil

scholarly journals Characterization of the Gut Microbial Community of Obese Patients Following a Weight-Loss Intervention Using Whole Metagenome Shotgun Sequencing

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149564 ◽  
Author(s):  
Sandrine Louis ◽  
Rewati-Mukund Tappu ◽  
Antje Damms-Machado ◽  
Daniel H. Huson ◽  
Stephan C. Bischoff
Keyword(s):  
Weight Loss ◽  
Microbial Community ◽  
Shotgun Sequencing ◽  
Obese Patients ◽  
Weight Loss Intervention ◽  
Gut Microbial Community

Characterization of a microbial community capable of nitrification at cold temperature

2010 ◽  
Vol 101 (2) ◽  
pp. 491-500 ◽  
Author(s):  
Thomas F. Ducey ◽  
Matias B. Vanotti ◽  
Anthony D. Shriner ◽  
Ariel A. Szogi ◽  
Aprel Q. Ellison
Keyword(s):  
Microbial Community ◽  
Cold Temperature

Characterization of microbial community in granular sludge treating brewery wastewater

2002 ◽  
Vol 36 (7) ◽  
pp. 1767-1775 ◽  
Author(s):  
Wen-Tso Liu ◽  
On-Chim Chan ◽  
Herbert H.P Fang
Keyword(s):  
Microbial Community ◽  
Granular Sludge ◽  
Brewery Wastewater

Characterization of an airborne microbial community: A case study in the archive of the University of Coimbra, Portugal

2013 ◽  
Vol 79 ◽  
pp. 36-41 ◽  
Author(s):  
Inês Nunes ◽  
Nuno Mesquita ◽  
Sandra Cabo Verde ◽  
Ana Maria Leitão Bandeira ◽  
Maria Manuela Carolino ◽  
...  
Keyword(s):  
Microbial Community ◽  
The University

scholarly journals Assessing high-throughput environmental DNA extraction methods for meta-barcode characterization of aquatic microbial communities

2018 ◽  
Vol 17 (1) ◽  
pp. 37-49 ◽  
Author(s):  
Abdolrazagh Hashemi Shahraki ◽  
Subba Rao Chaganti ◽  
Daniel Heath
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
High Throughput ◽  
Environmentally Friendly ◽  
Environmental Dna ◽  
Magnetic Bead ◽  
Extraction Methods ◽  
Environmental Research ◽  
Rrna Gene

Abstract The characterization of microbial community dynamics using genomic methods is rapidly expanding, impacting many fields including medical, ecological, and environmental research and applications. One of the biggest challenges for such studies is the isolation of environmental DNA (eDNA) from a variety of samples, diverse microbes, and widely variable community compositions. The current study developed environmentally friendly, user safe, economical, and high throughput eDNA extraction methods for mixed aquatic microbial communities and tested them using 16 s rRNA gene meta-barcoding. Five different lysis buffers including (1) cetyltrimethylammonium bromide (CTAB), (2) digestion buffer (DB), (3) guanidinium isothiocyanate (GITC), (4) sucrose lysis (SL), and (5) SL-CTAB, coupled with four different purification methods: (1) phenol-chloroform-isoamyl alcohol (PCI), (2) magnetic Bead-Robotic, (3) magnetic Bead-Manual, and (4) membrane-filtration were tested for their efficacy in extracting eDNA from recreational freshwater samples. Results indicated that the CTAB-PCI and SL-Bead-Robotic methods yielded the highest genomic eDNA concentrations and succeeded in detecting the core microbial community including the rare microbes. However, our study recommends the SL-Bead-Robotic eDNA extraction protocol because this method is safe, environmentally friendly, rapid, high-throughput and inexpensive.

scholarly journals Characterization of Microbial Community Structure in Gulf of Mexico Gas Hydrates: Comparative Analysis of DNA- and RNA-Derived Clone Libraries

2005 ◽  
Vol 71 (6) ◽  
pp. 3235-3247 ◽  
Author(s):  
Heath J. Mills ◽  
Robert J. Martinez ◽  
Sandra Story ◽  
Patricia A. Sobecky
Keyword(s):  
Phylogenetic Analysis ◽  
Microbial Community ◽  
Gulf Of Mexico ◽  
Gas Hydrate ◽  
Phylogenetic Analyses ◽  
Active Fraction ◽  
Clone Libraries ◽  
Dna And Rna ◽  
Rna And Dna

ABSTRACT The characterization of microbial assemblages within solid gas hydrate, especially those that may be physiologically active under in situ hydrate conditions, is essential to gain a better understanding of the effects and contributions of microbial activities in Gulf of Mexico (GoM) hydrate ecosystems. In this study, the composition of the Bacteria and Archaea communities was determined by 16S rRNA phylogenetic analyses of clone libraries derived from RNA and DNA extracted from sediment-entrained hydrate (SEH) and interior hydrate (IH). The hydrate was recovered from an exposed mound located in the northern GoM continental slope with a hydrate chipper designed for use on the manned-submersible Johnson Sea Link (water depth, 550 m). Previous geochemical analyses indicated that there was increased metabolic activity in the SEH compared to the IH layer (B. N. Orcutt, A. Boetius, S. K. Lugo, I. R. Macdonald, V. A. Samarkin, and S. Joye, Chem. Geol. 205:239-251). Phylogenetic analysis of RNA- and DNA-derived clones indicated that there was greater diversity in the SEH libraries than in the IH libraries. A majority of the clones obtained from the metabolically active fraction of the microbial community were most closely related to putative sulfate-reducing bacteria and anaerobic methane-oxidizing archaea. Several novel bacterial and archaeal phylotypes for which there were no previously identified closely related cultured isolates were detected in the RNA- and DNA-derived clone libraries. This study was the first phylogenetic analysis of the metabolically active fraction of the microbial community extant in the distinct SEH and IH layers of GoM gas hydrate.

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