A new fast screening method of multiple contingencies

2015 ◽  
pp. 37-42 ◽  
Keyword(s):  
Screening Method ◽  
Fast Screening

scholarly journals Immobilized artificial membrane (IAM) liquid chromatography as a model for antimicrobial peptide partitioning into cell membranes: An evaluation

Eureka ◽  
2010 ◽  
Vol 1 (1) ◽  
pp. 20-33
Author(s):  
Matthew Benesch ◽  
Ruthvnen Lewis ◽  
Ronald McElhaney
Keyword(s):  
Liquid Chromatography ◽  
Antimicrobial Peptides ◽  
Lipid Bilayers ◽  
Screening Method ◽  
Interfacial Region ◽  
Artificial Membrane ◽  
Lipid Packing ◽  
Immobilized Artificial Membrane ◽  
Fast Screening ◽  
Packing Densities

Non-covalent immobilized artificial membrane reverse-phase high performance liquid chromatography was previously evaluated as a means whereby elution times for antimicrobial peptides from columns mimicking the lipid bilayers of different membrane systems might be used as a fast-screening method to compare relative binding effectiveness. Such a system would aid in the development of antimicrobial peptides that bind preferentially to model pathogenic systems and leave the host’s membranes reasonably unaffected. A non-covalent approach allows for flexibility in membrane composition but was found to be inadequate for analysis of most peptides due to significant lipid loss at high acetonitrile concentrations. A covalent approach where phosphatidylcholine was amide-linked to the silica surface was examined to evaluate its use as a fast-screening method and compare its data to that collected from the non-covalent columns. Initial work with a 1-cm column proved ineffective due to problems with balancing flow rates with retention times, and work was shifted to a longer 10-cm column. Results suggested that peptides bind much more strongly to covalent columns than non-covalent ones, with the binding especially enhanced by the presence of cationic residues. These columns had lipid packing densities much lower than true membranes, indicating that the peptides were partitioning deep into the bonded phase of the columns rather than into the interfacial region of the phosphate head groups, as expected in situations of biologically-relevant lipid packing densities.

Development of a rapid screening protocol for selection of strains resistant to spray drying and storage in dry powder

2010 ◽  
Vol 1 (2) ◽  
pp. 165-174 ◽  
Author(s):  
S. Reimann ◽  
F. Grattepanche ◽  
C. Baggenstos ◽  
E. Rezzonico ◽  
B. Berger ◽  
...  
Keyword(s):  
Spray Drying ◽  
Bifidobacterium Longum ◽  
Survival Rates ◽  
Screening Method ◽  
Rapid Screening ◽  
Fast Screening ◽  
Screening Protocol ◽  
The Stability ◽  
And Storage ◽  
Selection Of

An efficient screening method for selection of Bifidobacterium longum strains resistant to spray drying and storage was developed based on randomly amplified polymorphic DNA (RAPD) for identification of the best survivors in mixed strains bacterial preparations. Three different primers were used to generate RAPD profiles of 22 B. longum strains. All strains were distinguished according to their RAPD profiles except for the strain NCC2705 and its H2O2 resistant derivative variant. The 22 strains were grouped in 3 batches of 7, 7 and 8 strains and subjected to spray drying and storage at 30 and 37 °C under anaerobic conditions. Batch survival rates after spray drying reached 17.1±4.4%. Strains showing the highest prevalence and/or resistance to storage at 37 °C were selected from individual batches for subsequent spray drying and storage testing. After 67 days of storage, NCC572 was identified as the dominant strain in powder. The stability of strain NCC572 was confirmed by performing single spray drying and storage tests. Out of 22 B. longum strains, a robust strain was identified by combining RAPD with a simultaneous screening test for survival under spray drying and storage. The method allowed a fast screening of B. longum strains in mixture for resistance to spray drying and storage compared to traditional screening procedures carried out with individual strains, in the same conditions. This approach could be applied to other stress conditions.

Development of a robust, fast screening method for the potentiometric determination of iodide in urine and salt samples

Talanta ◽  
2017 ◽  
Vol 167 ◽  
pp. 688-694 ◽  
Author(s):  
Ana Machado ◽  
Raquel B.R. Mesquita ◽  
Sara Oliveira ◽  
Adriano A. Bordalo
Keyword(s):  
Screening Method ◽  
Potentiometric Determination ◽  
Fast Screening

scholarly journals RADAR: A novel fast-screening method for reading difficulties with special focus on dyslexia

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182597 ◽  
Author(s):  
Ioannis Smyrnakis ◽  
Vassilios Andreadakis ◽  
Vassilios Selimis ◽  
Michail Kalaitzakis ◽  
Theodora Bachourou ◽  
...  
Keyword(s):  
Screening Method ◽  
Reading Difficulties ◽  
Special Focus ◽  
Fast Screening
Sign in / Sign up

Export Citation Format

Share Document