Statistical Optimization of Culture Media Components for Enhanced Production of Sophorolipids from Starmerella bombicola MTCC 1910 Using Waste Frying Sun Flower Oil

2015 ◽  
pp. 31-40
Keyword(s):  
Culture Media ◽  
Statistical Optimization ◽  
Enhanced Production ◽  
Starmerella Bombicola

scholarly journals EVALUATION OF DIFFERENT CULTURE MEDIA FOR ENHANCED PRODUCTION OF PSEUDOMONAS AERUGINOSA (MTCC NO 2453) BIOMASS AND ITS PROTEINS

2016 ◽  
Vol 8 (11) ◽  
pp. 120 ◽  
Author(s):  
P. Neeraja ◽  
T. Parthasarathy ◽  
M. Sudhakar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Production ◽  
Culture Media ◽  
Batch Cultivation ◽  
Luria Bertani ◽  
Shake Flask Culture ◽  
Cell Biomass ◽  
Enhanced Production ◽  
Anti Cancer ◽  
The Media

Objective: Microorganisms, especially bacteria and its proteins have proven to be potential anti-cancer agents as they selectively attack the tumor cells or tumor micro-environments. The extract of Pseudomonas aeruginosa found to contain proteins that have shown promising anticancer activity. In this work, it was attempted to increase the biomass and trigger the total protein fraction of Pseudomonas aeruginosa (MTCC 2453).Methods: The organism was cultivated in three different such as Luria-Bertani (LB) broth, minimal medium9 (M9), super broth medium (SB) and asparagine-proline (AP) broth. Asparagine proline broth was selected as it has shown high cell growth rate. The media was further optimized by the addition of NaHCO3 and copper sulphate to trigger the protein production. Optimized Aspergine proline broth has achieved highest cell biomass. After the shake flask culture, the overnight grown culture in optimized AP medium was further grown in a 5 L bioreactor by fed-batch cultivation to achieve higher cell densities.Results: The highest protein production was achieved at 40 ° C. Highest biomass and protein content was observed at pH 8 while lowest biomass was produced at pH 2. A gradual increase in biomass content observed from 12 h towards to 48 h.Conclusion: High biomass and proteins content and of Pseudomonas aeruginosa (MTCC 2453) can be produced in optimized asparagine-proline broth. Further the extract is purified to produce novel anti-cancer proteins.

scholarly journals Modification of Phytochemical Production and Antioxidant Activity of Dracocephalum Kotschyi Cells by Exposure to Static Magnetic Field and Magnetite Nanoparticles

2021 ◽  
Author(s):  
Marzieh Taghizadeh ◽  
Fatemeh Nasibi ◽  
Khosrow Khosrow Manouchehri kalantari ◽  
Mohsen Mohseni-Moghadam
Keyword(s):  
Magnetic Field ◽  
Static Magnetic Field ◽  
Magnetite Nanoparticles ◽  
High Performance ◽  
Culture Media ◽  
Cell Suspension Cultures ◽  
Simultaneous Exposure ◽  
Simultaneous Application ◽  
Enhanced Production ◽  
Dracocephalum Kotschyi

Abstract Dracocephlum kotschyi Boiss is a genus in Lamiaceae family and a medicinal herb native to Iran. The cell suspension cultures were treated by static magnetic field (SMF) and Fe3O4 magnetite nanoparticles (MNP) to understand the production yield of secondary metabolites. The treatment procedure was done by cultivating the cells either with 100 ppm MNP, SMFs, or simultaneous exposure to both MNP and SMFs. The SMF at 30 mT was uniformly applied to the cells either for 3 or 4 days with a 3 h per day or a 5 h per day intervals, respectively. The contents of phenolics and phytochemicals were then examined by high performance liquid chromatography and UV-Vis spectrophotometer. These treatments imposed oxidative stress and induced polyphenol oxidase and phenylalanine ammonia lyase, accompanied by enhanced production of phenolics, anthocyanins, flavonoids, and lignin. The highest membrane embrittlement and elicitation was found upon simultaneous application of the MNPs and SMFs, followed by the MNP and SMFs. The contents of naringin, rosmarinic acid, quercetin, thymol, carvacrol, apigenin, and rutin increased in the intracellular biomass of all treated cells and extracellular culture media. These findings propose the potential of these elicitors in simultaneous production and secretion of these phytochemicals into culture media.

IMPROVEDCandida methylicaFORMATE DEHYDROGENASE FERMENTATION THROUGH STATISTICAL OPTIMIZATION OF LOW-COST CULTURE MEDIA

2012 ◽  
Vol 42 (6) ◽  
pp. 507-519
Author(s):  
Mustafa Kahraman ◽  
Emel Bıçakçı Ordu ◽  
Koray Yeşiladalı ◽  
Nevin Gül Karagüler ◽  
Candan Tamerler
Keyword(s):  
Culture Media ◽  
Low Cost ◽  
Statistical Optimization
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