Research on Cu uptake and tolerance of several plants

2015 ◽  
pp. 41-44
Keyword(s):  
Cu Uptake

Ferrous Ions Inhibit Cu Uptake and Accumulation via Inducing Iron Plaque and Regulating Metabolism of Rice Plants Exposed to CuO Nanoparticles

2021 ◽  
Author(s):  
Peng Yuan ◽  
Cheng Peng ◽  
Jiyan Shi ◽  
Jianshe Liu ◽  
Dongqing Cai ◽  
...  
Keyword(s):  
Cuo Nanoparticles ◽  
Iron Plaque ◽  
Ferrous Ions ◽  
Rice Plants ◽  
Cu Uptake

Ferrous ions (Fe2+) in the rhizosphere play an indispensable role in the interaction between plants and nanoparticles (NPs). Here, we investigated the effect of Fe2+ (3 mM) on the dynamic...

scholarly journals Copper Transport and Disease: What Can We Learn from Organoids?

2019 ◽  
Vol 39 (1) ◽  
pp. 75-94 ◽  
Author(s):  
Hannah Pierson ◽  
Haojun Yang ◽  
Svetlana Lutsenko
Keyword(s):  
Three Dimensional ◽  
Transport Processes ◽  
Intestinal Epithelial ◽  
Metabolic Coupling ◽  
Significant Research ◽  
Cu Homeostasis ◽  
Vitro Model ◽  
Cu Uptake ◽  
Fat Processing

Many metals have biological functions and play important roles in human health. Copper (Cu) is an essential metal that supports normal cellular physiology. Significant research efforts have focused on identifying the molecules and pathways involved in dietary Cu uptake in the digestive tract. The lack of an adequate in vitro model for assessing Cu transport processes in the gut has led to contradictory data and gaps in our understanding of the mechanisms involved in dietary Cu acquisition. The recent development of organoid technology has provided a tractable model system for assessing the detailed mechanistic processes involved in Cu utilization and transport in the context of nutrition. Enteroid (intestinal epithelial organoid)-based studies have identified new links between intestinal Cu metabolism and dietary fat processing. Evidence for a metabolic coupling between the dietary uptake of Cu and uptake of fat (which were previously thought to be independent) is a new and exciting finding that highlights the utility of these three-dimensional primary culture systems. This review has three goals: ( a) to critically discuss the roles of key Cu transport enzymes in dietary Cu uptake; ( b) to assess the use, utility, and limitations of organoid technology in research into nutritional Cu transport and Cu-based diseases; and ( c) to highlight emerging connections between nutritional Cu homeostasis and fat metabolism.

AFLP Analysis and Improved Phytoextraction Capacity of Transgenic gshI-Poplar Clones (Populus x canescens L.) for Copper in vitro

2005 ◽  
Vol 60 (3-4) ◽  
pp. 300-306 ◽  
Author(s):  
Gábor Gyulai ◽  
Mervyn Humphreys ◽  
András Bittsánszky ◽  
Kirsten Skøtc ◽  
József Kiss ◽  
...  
Keyword(s):  
Culture Media ◽  
Leaf Disc ◽  
Aflp Analysis ◽  
Polymorphism Analysis ◽  
Tissue Culture Media ◽  
Dna Fragment ◽  
High Concentrations ◽  
Cu Uptake ◽  
Concentration Series

Abstract Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRIDMseI digestion. Four fragments generated by EcoAGTDMseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. \ canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10-1 to 10-5 ᴍ) incubated for 21 days on aseptic tissue culture media WPM containing 1 μᴍ Cu. Zn2+ caused phytotoxicity only at high concentrations (10-1 to 10-2 ᴍ). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.

Impact of ionophore monensin on performance and Cu uptake in earthworm Eisenia andrei exposed to copper-contaminated soil

Chemosphere ◽  
2016 ◽  
Vol 161 ◽  
pp. 119-126 ◽  
Author(s):  
Primož Zidar ◽  
Monika Kos ◽  
Katarina Vogel-Mikuš ◽  
Johannes Teun van Elteren ◽  
Marta Debeljak ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Eisenia Andrei ◽  
Copper Contaminated ◽  
Cu Uptake ◽  
Ionophore Monensin

scholarly journals Direct and subsequent effect of compost and poultry manure on the bioavailability of cadmium and copper and their uptake by oat biomass

2008 ◽  
Vol 54 (No. 7) ◽  
pp. 271-278 ◽  
Author(s):  
A. Hanč ◽  
P. Tlustoš ◽  
J. Száková ◽  
J. Habart ◽  
K. Gondek
Keyword(s):  
Correlation Coefficient ◽  
Avena Sativa ◽  
Organic Materials ◽  
Poultry Manure ◽  
Total Content ◽  
Cd Uptake ◽  
Dry Biomass ◽  
Subsequent Effect ◽  
Oat Biomass ◽  
Cu Uptake

Direct and subsequent influence of added organic materials on changes of cadmium and copper bioavailability in soil and their accumulation in aboveground oat (<I>Avena sativa</I> L.) biomass was studied in a three-year experiment. Mineral NPK, poultry manure and two types of composts were used as fertilizers. The average portion of available cadmium and copper from their total content in soil in all treatments was 0.94% and 0.25%, respectively. After application of poultry manure the concentration of available Cd increased during experimental years, contrary to Cu. The Cd uptake increased proportionally with available Cd content in soil almost in all treatments during experimental years (correlation coefficient <I>R</I> = 0.54; <I>P</I> < 0.05). The Cu uptake was however different than Cd uptake, mainly in the second and third year. The lowest uptake of Cu was found in the first experimental year and was in close positive correlation with yields of dry biomass.

scholarly journals Dissection of the relative contribution of the Schizosaccharomyces pombe Ctr4 and Ctr5 proteins to the copper transport and cell surface delivery functions

Microbiology ◽  
2011 ◽  
Vol 157 (4) ◽  
pp. 1021-1031 ◽  
Author(s):  
Jude Beaudoin ◽  
Dennis J. Thiele ◽  
Simon Labbé ◽  
Sergi Puig
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Schizosaccharomyces Pombe ◽  
Transmembrane Domain ◽  
Chimeric Proteins ◽  
High Affinity ◽  
Relative Contribution ◽  
Eukaryotic Organisms ◽  
Transport Complex ◽  
Cu Uptake

The Ctr1 family of proteins mediates high-affinity copper (Cu) acquisition in eukaryotic organisms. In the fission yeast Schizosaccharomyces pombe, Cu uptake is carried out by a heteromeric complex formed by the Ctr4 and Ctr5 proteins. Unlike human and Saccharomyces cerevisiae Ctr1 proteins, Ctr4 and Ctr5 are unable to function independently in Cu acquisition. Instead, both proteins physically interact with each other to form a Ctr4–Ctr5 heteromeric complex, and are interdependent for secretion to the plasma membrane and Cu transport activity. In this study, we used S. cerevisiae mutants that are defective in high-affinity Cu uptake to dissect the relative contribution of Ctr4 and Ctr5 to the Cu transport function. Functional complementation and localization assays show that the conserved Met-X3-Met motif in transmembrane domain 2 of the Ctr5 protein is dispensable for the functionality of the Ctr4–Ctr5 complex, whereas the Met-X3-Met motif in the Ctr4 protein is essential for function and for localization of the hetero-complex to the plasma membrane. Moreover, Ctr4/Ctr5 chimeric proteins reveal unique properties found either in Ctr4 or in Ctr5, and are sufficient for Cu uptake on the cell surface of Sch. pombe cells. Functional chimeras contain the Ctr4 central and Ctr5 carboxyl-terminal domains (CTDs). We propose that the Ctr4 central domain mediates Cu transport in this hetero-complex, whereas the Ctr5 CTD functions in the regulation of trafficking of the Cu transport complex to the cell surface.

Sign in / Sign up

Export Citation Format

Share Document