Leukocyte Transendothelial Migration: A Biophysical Event

2015 ◽  
pp. 235-258
Keyword(s):  
Transendothelial Migration ◽  
Leukocyte Transendothelial Migration

scholarly journals TRPC6 is the endothelial calcium channel that regulates leukocyte transendothelial migration during the inflammatory response

2015 ◽  
Vol 212 (11) ◽  
pp. 1883-1899 ◽  
Author(s):  
Evan W. Weber ◽  
Fei Han ◽  
Mohammad Tauseef ◽  
Lutz Birnbaumer ◽  
Dolly Mehta ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Response ◽  
Calcium Channel ◽  
Transient Receptor Potential ◽  
Leukocyte Adhesion ◽  
Transendothelial Migration ◽  
Dominant Negative ◽  
Ion Concentration ◽  
Free Calcium ◽  
Leukocyte Transendothelial Migration

Leukocyte transendothelial migration (TEM) is a tightly regulated, multistep process that is critical to the inflammatory response. A transient increase in endothelial cytosolic free calcium ion concentration (↑[Ca2+]i) is required for TEM. However, the mechanism by which endothelial ↑[Ca2+]i regulates TEM and the channels mediating this ↑[Ca2+]i are unknown. Buffering ↑[Ca2+]i in endothelial cells does not affect leukocyte adhesion or locomotion but selectively blocks TEM, suggesting a role for ↑[Ca2+]i specifically for this step. Transient receptor potential canonical 6 (TRPC6), a Ca2+ channel expressed in endothelial cells, colocalizes with platelet/endothelial cell adhesion molecule-1 (PECAM) to surround leukocytes during TEM and clusters when endothelial PECAM is engaged. Expression of dominant-negative TRPC6 or shRNA knockdown in endothelial cells arrests neutrophils apically over the junction, similar to when PECAM is blocked. Selectively activating endothelial TRPC6 rescues TEM during an ongoing PECAM blockade, indicating that TRPC6 functions downstream of PECAM. Furthermore, endothelial TRPC6 is required for trafficking of lateral border recycling compartment membrane, which facilitates TEM. Finally, mice lacking TRPC6 in the nonmyeloid compartment (i.e., endothelium) exhibit a profound defect in neutrophil TEM with no effect on leukocyte trafficking. Our findings identify endothelial TRPC6 as the calcium channel mediating the ↑[Ca2+]i required for TEM at a step downstream of PECAM homophilic interactions.

scholarly journals An Antibody to the Sixth Ig-like Domain of VCAM-1 Inhibits Leukocyte Transendothelial Migration without Affecting Adhesion

2012 ◽  
Vol 189 (9) ◽  
pp. 4592-4601 ◽  
Author(s):  
Sukmook Lee ◽  
Il-Hee Yoon ◽  
Aerin Yoon ◽  
Joan M. Cook-Mills ◽  
Chung-Gyu Park ◽  
...  
Keyword(s):  
Transendothelial Migration ◽  
Leukocyte Transendothelial Migration

scholarly journals Filamin B Mediates ICAM-1-driven Leukocyte Transendothelial Migration

2008 ◽  
Vol 283 (46) ◽  
pp. 31830-31839 ◽  
Author(s):  
Edwin Kanters ◽  
Jos van Rijssel ◽  
Paul J. Hensbergen ◽  
David Hondius ◽  
Frederik P. J. Mul ◽  
...  
Keyword(s):  
Transendothelial Migration ◽  
Leukocyte Transendothelial Migration ◽  
Filamin B

Emerging Topics in the Regulation of Leukocyte Transendothelial Migration

2005 ◽  
Vol 12 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Ravi M. Rao ◽  
Sunil K. Shaw ◽  
Michael Kim ◽  
F. William Luscinskas
Keyword(s):  
Transendothelial Migration ◽  
Leukocyte Transendothelial Migration ◽  
Emerging Topics
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