Baculovirus: A High-Capacity Vector for Versatile Gene Transfer

2015 ◽  
pp. 201-236
Author(s):  
Kari Airenne ◽  
Seppo Ylä-Herttuala
Keyword(s):  
Gene Transfer ◽  
High Capacity

scholarly journals Variables Affecting In Vivo Performance of High-Capacity Adenovirus Vectors

2002 ◽  
Vol 76 (4) ◽  
pp. 1600-1609 ◽  
Author(s):  
Gudrun Schiedner ◽  
Sabine Hertel ◽  
Marion Johnston ◽  
Volker Biermann ◽  
Volker Dries ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
High Capacity ◽  
Fold Increase ◽  
Expression Cassette ◽  
Matrix Attachment Region ◽  
Hepatic Expression ◽  
Vector Genome

ABSTRACT In high-capacity adenovirus (HC-Ad) vectors the size and/or composition of the vector genome influences vector stability during production and the expression profile following gene transfer. Typically, an HC-Ad vector will contain both a gene or an expression cassette and stuffer DNA that is required to balance the final vector genome to a size of between 27 and 36 kb. To gain an improved understanding of factors that may influence gene expression from HC-Ad vectors, we have generated a series of vectors that carry different combinations of human alpha-1 antitrypsin (hAAT) expression constructs and stuffer DNAs. Expression in vitro did not predict in vivo performance: all vectors expressed hAAT at similar levels when tested in cell culture. Hepatic expression was evaluated following in vivo gene transfer in C57BL/6J mice. hAAT levels obtained from genomic DNA were significantly higher than levels achieved with small cDNA expression cassettes. Expression was independent of the orientation and only marginally influenced by the location of the expression cassette within the vector genome. The use of lambda stuffer DNA resulted in low-level but stable expression for at least 3 months when higher doses were applied. A potential matrix attachment region element was identified within the hAAT gene and caused a 10-fold increase in expression when introduced in an HC-Ad vector genome carrying a phosphoglycerate kinase (pgk) hAAT cDNA construct. We also illustrate the influence of the promoter on anti-hAAT antibody formation in C57BL/6J mice: a human cytomegalovirus but not a pgk promoter resulted in an anti-hAAT antibody response. Thus, the overall design of HC-Ad vectors may significantly influence amounts and duration of gene expression at different levels.

Improved high-capacity adenoviral vectors for high-level neuron-restricted gene transfer to the CNS

2006 ◽  
Vol 136 (1-2) ◽  
pp. 30-37 ◽  
Author(s):  
Stephan Hermening ◽  
Sebastian Kügler ◽  
Mathias Bähr ◽  
Stefan Isenmann
Keyword(s):  
Gene Transfer ◽  
High Capacity ◽  
Adenoviral Vectors ◽  
High Level

scholarly journals Sustained Muscle Expression of Dystrophin from a High-Capacity Adenoviral Vector with Systemic Gene Transfer of T Cell Costimulatory Blockade

2004 ◽  
Vol 10 (4) ◽  
pp. 688-696 ◽  
Author(s):  
Zhilong Jiang ◽  
Gudrun Schiedner ◽  
Nico van Rooijen ◽  
Chau-Ching Liu ◽  
Stefan Kochanek ◽  
...  
Keyword(s):  
T Cell ◽  
Gene Transfer ◽  
Adenoviral Vector ◽  
High Capacity ◽  
Costimulatory Blockade

[17] High-capacity, helper-dependent, “gutless” adenoviral vectors for gene transfer into brain

2002 ◽  
pp. 292-311 ◽  
Author(s):  
P.R. Lowenstein ◽  
C.E. Thomas ◽  
P. Umana ◽  
C.A. Gerdes ◽  
T. Verakis ◽  
...  
Keyword(s):  
Gene Transfer ◽  
High Capacity ◽  
Adenoviral Vectors

A high-capacity, hybrid electro-microneedle for in-situ cutaneous gene transfer

Biomaterials ◽  
2011 ◽  
Vol 32 (30) ◽  
pp. 7705-7710 ◽  
Author(s):  
Kwang Lee ◽  
Jung Dong Kim ◽  
Chang Yoel Lee ◽  
Song Her ◽  
Hyungil Jung
Keyword(s):  
Gene Transfer ◽  
High Capacity
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