Applications of High Pressure as a Nonthermal Fermentation Control Technique

2006 ◽  
Author(s):  
Fujian Zhou ◽  
Yiping Zong ◽  
Yuzhang Liu ◽  
Xianyou Yang ◽  
Chunming Xiong ◽  
...  

2006 ◽  
Vol 16 (2) ◽  
pp. 167-178 ◽  
Author(s):  
Akihiko SASAGAWA ◽  
Jun HOSHINO ◽  
Atsushi KOBAYASHI ◽  
Tadayuki NISHIUMI ◽  
Atsushi SUZUKI ◽  
...  

Author(s):  
Julien Marty ◽  
William Riéra ◽  
Lionel Castillon

In the present study, several control devices have been investigated in the framework of a high pressure compressor rotor using RANS simulations. The analysis of performance maps and of flow predictions leads to select the injection device located up-stream of the rotor tip, at the shroud in order to control the tip leakage flow. The high loss levels are reduced and the radial and azimuthal extension of the high loss area is smaller. Then the chosen flow control technique has been simulated using URANS and ZDES with the IGV passing wakes. The injection technique reduces the loss area and level, energizing and stabilizing the tip leakage vortex thanks to high momentum. So the vortex disruption is removed or at least delayed. Moreover, the influence of IGV passing wakes is reduced.


Author(s):  
Jian Liu ◽  
Wei-Yang Qiao ◽  
Peng Huang ◽  
Wei-Jie Chen

This paper aims to numerically and experimentally analyze the effect of the micro-jet on the suction side (SS) of the transonic high-pressure turbine cascade, which could enable the possibility of the mass matching for turbine of the variable cycle engine (VCE) when it transforms working conditions according to flight demands. The experimental study had been conducted in a transonic and supersonic cascade wind tunnel to observe the influence of this kind of flow control technique on aerodynamic performance, operated at different exit Mach numbers. The nozzle guide vane with convergent-divergent passage was investigated in this paper. Two-dimensional steady simulations were performed to investigate the influence of the flow control method to exploit the potential of the application of this method in the design of high-pressure (HP) turbine for VCE. Results of the numerical simulations and experiments indicated that micro-jet on the SS of the turbine blade would generate a separation zone near the jet slot, which would reduce the genuine throat area of the turbine passage and thus change the mass flow rate through the passage of the turbine cascade significantly. Also, velocity at the passage throat would be reduced due to the application of SS micro-jet, which would also contribute to the reduction of the mass flow rate through the turbine passage. However, extended total pressure loss was introduced with SS micro-jet.


2013 ◽  
Vol 31 (12) ◽  
pp. 1306-1311
Author(s):  
D. Jingen ◽  
Z. Xun ◽  
C. Yu ◽  
Z. Wenlong ◽  
L. Shujie

Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.


Author(s):  
Robert Corbett ◽  
Delbert E. Philpott ◽  
Sam Black

Observation of subtle or early signs of change in spaceflight induced alterations on living systems require precise methods of sampling. In-flight analysis would be preferable but constraints of time, equipment, personnel and cost dictate the necessity for prolonged storage before retrieval. Because of this, various tissues have been stored in fixatives and combinations of fixatives and observed at various time intervals. High pressure and the effect of buffer alone have also been tried.Of the various tissues embedded, muscle, cartilage and liver, liver has been the most extensively studied because it contains large numbers of organelles common to all tissues (Fig. 1).


Author(s):  
R.E. Crang ◽  
M. Mueller ◽  
K. Zierold

Obtaining frozen-hydrated sections of plant tissues for electron microscopy and microanalysis has been considered difficult, if not impossible, due primarily to the considerable depth of effective freezing in the tissues which would be required. The greatest depth of vitreous freezing is generally considered to be only 15-20 μm in animal specimens. Plant cells are often much larger in diameter and, if several cells are required to be intact, ice crystal damage can be expected to be so severe as to prevent successful cryoultramicrotomy. The very nature of cell walls, intercellular air spaces, irregular topography, and large vacuoles often make it impractical to use immersion, metal-mirror, or jet freezing techniques for botanical material.However, it has been proposed that high-pressure freezing (HPF) may offer an alternative to the more conventional freezing techniques, inasmuch as non-cryoprotected specimens may be frozen in a vitreous, or near-vitreous state, to a radial depth of at least 0.5 mm.


Author(s):  
William P. Sharp ◽  
Robert W. Roberson

The aim of ultrastructural investigation is to analyze cell architecture and relate a functional role(s) to cell components. It is known that aqueous chemical fixation requires seconds to minutes to penetrate and stabilize cell structure which may result in structural artifacts. The use of ultralow temperatures to fix and prepare specimens, however, leads to a much improved preservation of the cell’s living state. A critical limitation of conventional cryofixation methods (i.e., propane-jet freezing, cold-metal slamming, plunge-freezing) is that only a 10 to 40 μm thick surface layer of cells can be frozen without distorting ice crystal formation. This problem can be allayed by freezing samples under about 2100 bar of hydrostatic pressure which suppresses the formation of ice nuclei and their rate of growth. Thus, 0.6 mm thick samples with a total volume of 1 mm3 can be frozen without ice crystal damage. The purpose of this study is to describe the cellular details and identify potential artifacts in root tissue of barley (Hordeum vulgari L.) and leaf tissue of brome grass (Bromus mollis L.) fixed and prepared by high-pressure freezing (HPF) and freeze substitution (FS) techniques.


Author(s):  
E. F. Koch

Because of the extremely rigid lattice structure of diamond, generating new dislocations or moving existing dislocations in diamond by applying mechanical stress at ambient temperature is very difficult. Analysis of portions of diamonds deformed under bending stress at elevated temperature has shown that diamond deforms plastically under suitable conditions and that its primary slip systems are on the ﹛111﹜ planes. Plastic deformation in diamond is more commonly observed during the high temperature - high pressure sintering process used to make diamond compacts. The pressure and temperature conditions in the sintering presses are sufficiently high that many diamond grains in the sintered compact show deformed microtructures.In this report commercially available polycrystalline diamond discs for rock cutting applications were analyzed to study the deformation substructures in the diamond grains using transmission electron microscopy. An individual diamond particle can be plastically deformed in a high pressure apparatus at high temperature, but it is nearly impossible to prepare such a particle for TEM observation, since any medium in which the diamond is mounted wears away faster than the diamond during ion milling and the diamond is lost.


Sign in / Sign up

Export Citation Format

Share Document