Usage of Speed Sedimentation Method for Enzyme Degradation of Chitosan

2014 ◽  
pp. 131-138 ◽  
Keyword(s):  
Sedimentation Method ◽  
Enzyme Degradation

Multi-chain action of exo-D-galacturonanase from carrot

1983 ◽  
Vol 48 (12) ◽  
pp. 3579-3588
Author(s):  
Kveta Heinrichová ◽  
Jana Perečková
Keyword(s):  
Optimal Condition ◽  
Degradation Products ◽  
Degradation Kinetics ◽  
Hydrolytic Cleavage ◽  
Chain Mechanism ◽  
Modes Of Action ◽  
Enzyme Degradation ◽  
Good Agreement

Two possible modes of action of exo-D-galacturonanase from carrot (E.C. 3.2.1.67) were investigated; this enzyme catalyses the sequential hydrolytic cleavage of pectants and oligogalacturonans by a terminal action from the nonreducing end of the molecule. The experiments indicate that the investigated exo-D-galacturonanase degrades these substrates by a predominantly multi-chain mechanism. Distribution of degradation products of oligomeric substrates (hexa- and pentagalacturonide) under an optimal condition for the action of the enzyme (pH and temperature) indicates that a multi-chain enzyme attack with a prevalent simple collision is involved. Results of the enzyme degradation kinetics are in a good agreement with the above-mentioned presumption.

scholarly journals Evaluation of Cellulose Nanofi bers by Using Sedimentation Method

2019 ◽  
Vol 73 (5) ◽  
pp. 461-469 ◽  
Author(s):  
Akio Kumagai ◽  
Takashi Endo ◽  
Maki Adachi
Keyword(s):  
Sedimentation Method

scholarly journals Comparison of two coprological methods for the veterinary diagnosis of fasciolosis

2005 ◽  
Vol 57 (2) ◽  
pp. 181-185 ◽  
Author(s):  
F. Kleiman ◽  
S. Pietrokovsky ◽  
S. Gil ◽  
C. Wisnivesky-Colli
Keyword(s):  
Endemic Area ◽  
Diagnostic Method ◽  
Staining Method ◽  
Fasciola Hepatica ◽  
False Negative ◽  
Sample Processing ◽  
Hepatica Infection ◽  
Sedimentation Method ◽  
Faecal Samples ◽  
Reliable Diagnostic Method

The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method.

scholarly journals Properties of phosphoenolpyruvate carboxykinase (guanosine triphosphate) synthesized in hepatoma cells in the presence of amino acid analogues

1975 ◽  
Vol 146 (3) ◽  
pp. 585-593 ◽  
Author(s):  
S E Knowles ◽  
J M Gunn ◽  
L Reshef ◽  
R W Hanson ◽  
F J Ballard
Keyword(s):  
Amino Acid ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Hepatoma Cells ◽  
Dibutyryl Cyclic Amp ◽  
Enzyme Degradation ◽  
Amino Acid Analogues ◽  
Enzyme Molecules ◽  
Natural Amino Acids

1. Phosphoenolpyruvate carboxykinase (GTP) was induced by a combination of dibutyryl cyclic AMP, theophyline and dexamethasone in Reuber H35 hepatoma cells under conditions where an amino acid in the medium was replaced by an appropriate analogue. 2. With canavanine replacing arginine or with 5-fluorotryptophan or 6-fluorotryptophan replacing tryptophan the induced enzyme had a lower catalytic activity-relative to antibody reactivity. 3. These aberrant enzyme molecules were heat-labile in vitro. 4. Measurements of enzyme degradation in vivo indicated that the canavanine-containing enzyme and the 6-fluorotryptophan-containing enzyme were degraded more rapidly than the enzyme containing all natural amino acids.

scholarly journals Sedimentation Method to Evaluate PFI Mill Beating Degree of Wood Pulp Fibers

2004 ◽  
Vol 60 (4) ◽  
pp. 112-117 ◽  
Author(s):  
Bo Chen ◽  
Daisuke Tatsumi ◽  
Takayoshi Matsumoto
Keyword(s):  
Wood Pulp ◽  
Pulp Fibers ◽  
Sedimentation Method

scholarly journals Enzyme degradation mechanism of white rot fungi and its research progress on Refractory Wastewater

2021 ◽  
Vol 237 ◽  
pp. 01002
Author(s):  
ShiYuan Huang ◽  
Sheng Li ◽  
ZhenYu Wang ◽  
SenHuan Lin ◽  
Jian Deng
Keyword(s):  
Phenolic Compounds ◽  
Enzymatic Degradation ◽  
Enzyme System ◽  
Degradation Mechanism ◽  
White Rot Fungi ◽  
White Rot ◽  
Research Progress ◽  
Degrading Enzyme ◽  
Research Status ◽  
Enzyme Degradation

The lignin-degrading enzyme system of white rot fungi is highly efficient and non-specific, and can degrade a variety of pollutants, including dyes, phenolic compounds and pesticides.The article presents an overview of the mechanism of enzymatic degradation of white rot fungi and its research status in several refractory wastewater were described.

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