Effect of Nanostructures on Cellular Responses and Tissue Regeneration

2014 ◽  
pp. 143-144
Keyword(s):  
Tissue Regeneration ◽  
Cellular Responses

Chemically modified glycosaminoglycan derivatives as building blocks for biomaterial coatings and hydrogels

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Matthias Schnabelrauch ◽  
Jürgen Schiller ◽  
Stephanie Möller ◽  
Dieter Scharnweber ◽  
Vera Hintze
Keyword(s):  
Extracellular Matrix ◽  
Tissue Regeneration ◽  
Building Blocks ◽  
Cellular Responses ◽  
Chemical Methods ◽  
Chemical Functionalization ◽  
Chemically Modified ◽  
Functional Biomaterials

Abstract Tissue regeneration is regulated by the cellular microenvironment, e.g. the extracellular matrix. Here, sulfated glycosaminoglycans (GAG), are of vital importance interacting with mediator proteins and influencing their biological activity. Hence, they are promising candidates for controlling tissue regeneration. This review addresses recent achievements regarding chemically modified GAG as well as collagen/GAG-based coatings and hydrogels including (i) chemical functionalization strategies for native GAG, (ii) GAG-based biomaterial strategies for controlling cellular responses, (iii) (bio)chemical methods for characterization and iv) protein interaction profiles and attained tissue regeneration in vitro and in vivo. The potential of GAG for bioinspired, functional biomaterials is highlighted.

scholarly journals A novel jet-based nano-hydroxyapatite patterning technique for osteoblast guidance

2009 ◽  
Vol 7 (42) ◽  
pp. 189-197 ◽  
Author(s):  
Xiang Li ◽  
Garrit Koller ◽  
Jie Huang ◽  
Lucy Di Silvio ◽  
Tara Renton ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Crucial Role ◽  
Cell Attachment ◽  
Cellular Responses ◽  
Bone Tissue Regeneration ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Commercially Pure ◽  
Patterning Technique

Surface topography is well known to play a crucial role in influencing cellular responses to an implant material and is therefore important in bone tissue regeneration. A novel jet-based patterning technique, template-assisted electrohydrodynamic atomization spraying, was recently devised to control precisely the surface structure as well as its dimensions. In the present study, a detailed investigation of this patterning process was carried out. A range of nano-hydroxyapatite (nHA) line-shaped patterns <20 µm in width were successfully deposited on a commercially pure Ti surface by controlling the flow of an nHA suspension in an electric field. In vitro studies showed that the nHA patterns generated are capable of regulating the human osteoblast cell attachment and orientation.

Applications of nanobioceramics to healthcare technology

2013 ◽  
Vol 2 (6) ◽  
pp. 679-697 ◽  
Author(s):  
Jason Feng ◽  
Eng San Thian
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cellular Responses ◽  
Biological Properties ◽  
Advanced Materials ◽  
Healthcare Industry ◽  
Chemical Similarity ◽  
Current Form ◽  
Biological Solutions

AbstractThe development of functional, biological solutions to repair or replace damaged tissues and organs is the goal of tissue engineering. This involves an interplay of cells, scaffolds and biomolecules that would generate a favourable response when implanted into patients, thus restoring functions lost or impaired due to injuries or diseases. Advances in nanotechnology have enabled the design and fabrication of novel materials at the nanometre scale. Hailed as the next generation of advanced materials, nanomaterials possess advantages of being biochemically and nanostructurally similar to that of physiological tissues. Moreover, nanotopological cues are incorporated, ensuring appropriate cellular responses, thereby enhancing the success of tissue regeneration. Nanobioceramics play a crucial role in bone tissue engineering due to its close chemical similarity to physiological bone and excellent biocompatibility. In addition, nanoscale engineering of these materials has the ability to enhance mechanical and biological properties. This review will begin with an introduction to nanomaterials and its associated considerations that should be taken into account. Next, the role of nanobioceramics achieving these considerations will be discussed. An overview of the current form of nanobioceramics being developed will be provided, concluding with an outlook of nanobioceramics for the healthcare industry.

scholarly journals Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Tomoya Hasegawa ◽  
Christopher J Hall ◽  
Philip S Crosier ◽  
Gembu Abe ◽  
Koichi Kawakami ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Tissue Regeneration ◽  
Essential Role ◽  
Tissue Injury ◽  
Interleukin 1 ◽  
Cellular Responses ◽  
Interleukin 1 Beta ◽  
Principal Source ◽  
Regenerative Cells ◽  
Underlying Processes

Cellular responses to injury are crucial for complete tissue regeneration, but their underlying processes remain incompletely elucidated. We have previously reported that myeloid-defective zebrafish mutants display apoptosis of regenerative cells during fin fold regeneration. Here, we found that the apoptosis phenotype is induced by prolonged expression of interleukin 1 beta (il1b). Myeloid cells are considered to be the principal source of Il1b, but we show that epithelial cells express il1b in response to tissue injury and initiate the inflammatory response, and that its resolution by macrophages is necessary for survival of regenerative cells. We further show that Il1b plays an essential role in normal fin fold regeneration by regulating expression of regeneration-induced genes. Our study reveals that proper levels of Il1b signaling and tissue inflammation, which are tuned by macrophages, play a crucial role in tissue regeneration.

The Response of Testis Cells to Low Dose X-Irradiation

1981 ◽  
Vol 39 ◽  
pp. 542-543
Author(s):  
D. E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
Joann Stevenson ◽  
S. Black
Keyword(s):  
Electron Microscopy ◽  
Stem Cell ◽  
Light Microscopy ◽  
Dose Level ◽  
Cross Sections ◽  
Low Dose ◽  
Light And Electron Microscopy ◽  
Cellular Responses ◽  
Post Irradiation ◽  
X Irradiation

The response of spermatogonial cells to X-irradiation is well documented. It has been shown that there is a radiation resistent stem cell (As) which, after irradiation, replenishes the seminiferous epithelium. Most investigations in this area have dealt with radiation dosages of 100R or more. This study was undertaken to observe cellular responses at doses less than 100R of X-irradiation utilizing a system in which the tissue can be used for light and electron microscopy.Brown B6D2F1 mice aged 16 weeks were exposed to X-irradiation (225KeV; 15mA; filter 0.35 Cu; 50-60 R/min). Four mice were irradiated at each dose level between 1 and 100 rads. Testes were removed 3 days post-irradiation, fixed, and embedded. Sections were cut at 2 microns for light microscopy. After staining, surviving spermatogonia were identified and counted in tubule cross sections. The surviving fraction of spermatogonia compared to control, S/S0, was plotted against dose to give the curve shown in Fig. 1.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

192: Tubularized Urethral Replacement: What is the Maximum Distance for Normal Tissue Regeneration?

2004 ◽  
Vol 171 (4S) ◽  
pp. 51-51
Author(s):  
Roger E. De Filippo ◽  
Hans G. Pohl ◽  
James J. Yoo ◽  
Anthony Atala
Keyword(s):  
Tissue Regeneration ◽  
Normal Tissue ◽  
Maximum Distance

Metalloproteinases as mediators of tissue regeneration in the peripheral nerve: an important role for metalloproteinase-2

2007 ◽  
Vol 34 (S 2) ◽  
Author(s):  
A Köhne ◽  
HC Lehmann ◽  
O Kiehl ◽  
G Meyer zu Hörste ◽  
HP Hartung ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Tissue Regeneration
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