Spatiotemporal Detection of NADH-Linked Enzyme Activities in Single Cell Metabolism

2014 ◽  
pp. 230-243
Keyword(s):  
Single Cell ◽  
Enzyme Activities ◽  
Cell Metabolism

scholarly journals The Development of Single-Cell Metabolism and Its Role in Studying Cancer Emergent Properties

2022 ◽  
Vol 11 ◽  
Author(s):  
Dingju Wei ◽  
Meng Xu ◽  
Zhihua Wang ◽  
Jingjing Tong
Keyword(s):  
Tumor Cell ◽  
Single Cell ◽  
Immune Escape ◽  
Cell Metabolism ◽  
Single Cells ◽  
Metabolic Reprogramming ◽  
Biological Information ◽  
Emergent Properties ◽  
Anti Cancer ◽  
Cell Metabolomics

Metabolic reprogramming is one of the hallmarks of malignant tumors, which provides energy and material basis for tumor rapid proliferation, immune escape, as well as extensive invasion and metastasis. Blocking the energy and material supply of tumor cells is one of the strategies to treat tumor, however tumor cell metabolic heterogeneity prevents metabolic-based anti-cancer treatment. Therefore, searching for the key metabolic factors that regulate cell cancerous change and tumor recurrence has become a major challenge. Emerging technology––single-cell metabolomics is different from the traditional metabolomics that obtains average information of a group of cells. Single-cell metabolomics identifies the metabolites of single cells in different states by mass spectrometry, and captures the molecular biological information of the energy and substances synthesized in single cells, which provides more detailed information for tumor treatment metabolic target screening. This review will combine the current research status of tumor cell metabolism with the advantages of single-cell metabolomics technology, and explore the role of single-cell sequencing technology in searching key factors regulating tumor metabolism. The addition of single-cell technology will accelerate the development of metabolism-based anti-cancer strategies, which may greatly improve the prognostic survival rate of cancer patients.

Metabolic and cyclic nucleotide enzyme activities in muscle and nonmuscle cells of rat heart during perinatal development

1985 ◽  
Vol 63 (1) ◽  
pp. 78-81 ◽  
Author(s):  
Russell T. Dowell
Keyword(s):  
Adenylate Cyclase ◽  
Muscle Cell ◽  
Cyclic Nucleotide ◽  
Enzyme Activities ◽  
Rat Heart ◽  
Citrate Synthase ◽  
Heart Function ◽  
Cell Metabolism ◽  
Adult Rats ◽  
Nonmuscle Cells

Enzyme activities related to aerobic metabolism and cyclic nucleotides were evaluated in muscle and nonmuscle cells of rat heart. The perinatal period from weaning to adult was studied. Malate dehydrogenase, citrate synthase, and 3-hydroxyacyl-CoA dehydrogenase activities of nonmuscle cells equal or exceed muscle cell activities in the weanling heart. Aerobic enzymes remain unchanged in nonmuscle cells during growth; however, muscle cell activities are enhanced. Adenylate cyclase and guanylate cyclase activities are higher in heart homogenates of weanling than adult rats. Despite elevated adenylate cyclase activity, cyclic AMP levels are identical in weanling and adult rats. Cyclic GMP levels are twofold higher in weanling than in adult rats. Muscle cell metabolism and cyclic nucleotide levels are associated with growth-related changes in heart function and cellularity, respectively.

scholarly journals A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single-Cell Metabolism in Droplet-Based Microfluidics

2016 ◽  
Vol 55 (30) ◽  
pp. 8581-8584 ◽  
Author(s):  
Fabio Del Ben ◽  
Matteo Turetta ◽  
Giorgia Celetti ◽  
Aigars Piruska ◽  
Michela Bulfoni ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Metabolism

Single-cell metabolism predicts drug response in patient-derived pancreatic cancer organoids (Conference Presentation)

2018 ◽  
Author(s):  
Joe T. Sharick ◽  
Alexander A. Parikh ◽  
Jillian K. Johnson ◽  
Lingjun Li ◽  
Cheri A. Pasch ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Single Cell ◽  
Drug Response ◽  
Cell Metabolism
Sign in / Sign up

Export Citation Format

Share Document