Fluorescence detection and lifetime imaging with stimulated emission

2014 ◽  
pp. 161-178
Author(s):  
Po-­Yen Lin ◽  
Jianhong Ge ◽  
Cuifang Kuang ◽  
Fu-­Jen Kao
Keyword(s):  
Fluorescence Detection ◽  
Stimulated Emission ◽  
Lifetime Imaging

scholarly journals Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

2012 ◽  
Vol 20 (27) ◽  
pp. 28216 ◽  
Author(s):  
Jianhong Ge ◽  
Cuifang Kuang ◽  
Shin-Shian Lee ◽  
Fu-Jen Kao
Keyword(s):  
Diode Laser ◽  
Fluorescence Lifetime ◽  
Stimulated Emission ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Supercontinuum Stimulated Emission Depletion Fluorescence Lifetime Imaging

2012 ◽  
Vol 116 (27) ◽  
pp. 7821-7826 ◽  
Author(s):  
Michael D. Lesoine ◽  
Sayantan Bose ◽  
Jacob W. Petrich ◽  
Emily A. Smith
Keyword(s):  
Fluorescence Lifetime ◽  
Stimulated Emission ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Stimulated Emission Depletion

Enhancing stimulated emission-based fluorescence detection with interferometric setup

2014 ◽  
Author(s):  
Shen-Shou Max Chung ◽  
Jia-Hui Deng ◽  
Po-Lin Lin ◽  
Fu-Jen Kao
Keyword(s):  
Fluorescence Detection ◽  
Stimulated Emission

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging

2008 ◽  
Vol 33 (2) ◽  
pp. 113 ◽  
Author(s):  
Egidijus Auksorius ◽  
Bosanta R. Boruah ◽  
Christopher Dunsby ◽  
Peter M. P. Lanigan ◽  
Gordon Kennedy ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Stimulated Emission ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Stimulated Emission Depletion

scholarly journals Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications

2019 ◽  
Vol 12 (05) ◽  
pp. 1940003 ◽  
Author(s):  
Mengjie Zhao ◽  
Shuai Ye ◽  
Xiao Peng ◽  
Jun Song ◽  
Junle Qu
Keyword(s):  
Quantum Dots ◽  
Fluorescence Lifetime ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Lateral Resolution ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
High Fluorescence Intensity ◽  
High Fluorescence

Inorganic quantum dots (QDs) have excellent optical properties, such as high fluorescence intensity, excellent photostability and tunable emission wavelength, etc., facilitating them to be used as labels and probes for bioimaging. In this study, CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope (FLIM) and stimulated emission depletion (STED) nanoscopy imaging. The emission peak of CdSe@ZnS QDs centered at 526[Formula: see text]nm with a narrow width of 19[Formula: see text]nm and the photoluminescence quantum yield (PLQY) was 64%. The QDs presented excellent anti-photobleaching property which can be irradiated for 400[Formula: see text]min by STED laser with 39.8[Formula: see text]mW. The lateral resolution of 42.0[Formula: see text]nm is demonstrated for single QDs under STED laser (27.5[Formula: see text]mW) irradiation. Furthermore, the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5[Formula: see text]nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD probes. Meanwhile, Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM, and their fluorescence lifetime was around 3.1[Formula: see text]ns, consistent with the in vitro value, suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

2013 ◽  
Author(s):  
Jianhong Ge ◽  
Yixin Wang ◽  
Cuifang Kuang ◽  
Shin-Shian Lee ◽  
Nirmal Mazumder ◽  
...  
Keyword(s):  
Diode Laser ◽  
Fluorescence Lifetime ◽  
Stimulated Emission ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging
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