Single-­molecule imaging with quantum dots

2014 ◽  
pp. 135-160 ◽  
Author(s):  
Mohammad Zahid ◽  
Andrew Smith
Keyword(s):  
Quantum Dots ◽  
Single Molecule ◽  
Single Molecule Imaging

scholarly journals Single Molecule Imaging with Stable 6 NM Quantum Dots

2012 ◽  
Vol 102 (3) ◽  
pp. 182a
Author(s):  
En Cai ◽  
Katie Wilson ◽  
Pinghua Ge ◽  
Marco Tjioe ◽  
David G. Fernig ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Single Molecule ◽  
Single Molecule Imaging

Compact Halo-Ligand-Conjugated Quantum Dots for Multicolored Single-Molecule Imaging of Overcrowding GPCR Proteins on Cell Membranes

Small ◽  
2014 ◽  
Vol 11 (12) ◽  
pp. 1396-1401 ◽  
Author(s):  
Akihito Komatsuzaki ◽  
Tatsuya Ohyanagi ◽  
Yoshikazu Tsukasaki ◽  
Yukihiro Miyanaga ◽  
Masahiro Ueda ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Single Molecule ◽  
Cell Membranes ◽  
Single Molecule Imaging

scholarly journals Stable, small, specific, low-valency quantum dots for single-molecule imaging

Nanoscale ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 4406-4414 ◽  
Author(s):  
Jungmin Lee ◽  
Xinyi Feng ◽  
Ou Chen ◽  
Moungi G. Bawendi ◽  
Jun Huang
Keyword(s):  
Quantum Dots ◽  
Single Cell ◽  
Single Molecule ◽  
Single Molecule Imaging

Small, specific, low-valency quantum dots for single-cell and single-molecule imaging.

scholarly journals Imaging: Compact Halo-Ligand-Conjugated Quantum Dots for Multicolored Single-Molecule Imaging of Overcrowding GPCR Proteins on Cell Membranes (Small 12/2015)

Small ◽  
2015 ◽  
Vol 11 (12) ◽  
pp. 1358-1358
Author(s):  
Akihito Komatsuzaki ◽  
Tatsuya Ohyanagi ◽  
Yoshikazu Tsukasaki ◽  
Yukihiro Miyanaga ◽  
Masahiro Ueda ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Single Molecule ◽  
Cell Membranes ◽  
Single Molecule Imaging

scholarly journals Multispecies Single Molecule Imaging With Quantum Dots

2009 ◽  
Vol 96 (3) ◽  
pp. 283a
Author(s):  
Christoffer Lagerholm ◽  
Eva Arnspang Christensen ◽  
Mathias Lysemose Clausen ◽  
Pasad Kulatunga
Keyword(s):  
Quantum Dots ◽  
Single Molecule ◽  
Single Molecule Imaging

Compact and stable SNAP ligand-conjugated quantum dots as a fluorescent probe for single-molecule imaging of dynein motor protein

2015 ◽  
Vol 51 (80) ◽  
pp. 14836-14839 ◽  
Author(s):  
Tatsuya Ohyanagi ◽  
Tomohiro Shima ◽  
Yasushi Okada ◽  
Yoshikazu Tsukasaki ◽  
Akihito Komatsuzaki ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescent Probe ◽  
Single Molecule ◽  
Colloidal Stability ◽  
Motor Protein ◽  
Single Molecule Imaging ◽  
Dynein Motor ◽  
Wide Range

Compact SNAP ligand-conjugated quantum dots (<10 nm) with high colloidal stability over a wide range of pH (5–9) are presented as a fluorescent probe for single-molecule imaging of dynein motor protein.

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

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