Recent Developments in Fourier Transform Infrared (FTIR) Microspectroscopic Methods for Biomedical Analyses: From Single-Point Detection to Two-Dimensional Imaging

2014 ◽  
pp. 826-841
Keyword(s):  
Fourier Transform ◽  
Single Point ◽  
Fourier Transform Infrared ◽  
Two Dimensional ◽  
Recent Developments ◽  
Point Detection

Two-Dimensional Fourier Transform Infrared-Visible and Infrared-Raman Spectroscopies

2015 ◽  
pp. 503-505 ◽  
Author(s):  
Trevor L. Courtney ◽  
Zachary W. Fox ◽  
Karla M. Slenkamp ◽  
Michael S. Lynch ◽  
Munira Khalil
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Two Dimensional

scholarly journals Evaluation of Melanoma (SK-MEL-2) Cell Growth between Three-Dimensional (3D) and Two-Dimensional (2D) Cell Cultures with Fourier Transform Infrared (FTIR) Microspectroscopy

2020 ◽  
Vol 21 (11) ◽  
pp. 4141 ◽  
Author(s):  
Tarapong Srisongkram ◽  
Natthida Weerapreeyakul ◽  
Kanjana Thumanu
Keyword(s):  
Cell Death ◽  
Fourier Transform ◽  
Three Dimensional ◽  
Central Area ◽  
Hierarchical Cluster ◽  
Fourier Transform Infrared ◽  
Two Dimensional ◽  
Ftir Microspectroscopy ◽  
Dna And Rna ◽  
3D Spheroids

Fourier transform infrared (FTIR) microspectroscopy was used to evaluate the growth of human melanoma cells (SK-MEL-2) in two-dimensional (2D) versus three-dimensional (3D) spheroid culture systems. FTIR microspectroscopy, coupled with multivariate analysis, could be used to monitor the variability of spheroid morphologies prepared from different cell densities. The characteristic shift in absorbance bands of the 2D cells were different from the spectra of cells from 3D spheroids. FTIR microspectroscopy can also be used to monitor cell death similar to fluorescence cell staining in 3D spheroids. A change in the secondary structure of protein was observed in cells from the 3D spheroid versus the 2D culture system. FTIR microspectroscopy can detect specific alterations in the biological components inside the spheroid, which cannot be detected using fluorescence cell death staining. In the cells from 3D spheroids, the respective lipid, DNA, and RNA region content represent specific markers directly proportional to the spheroid size and central area of necrotic cell death, which can be confirmed using unsupervised PCA and hierarchical cluster analysis. FTIR microspectroscopy could be used as an alternative tool for spheroid cell culture discrimination, and validation of the usual biochemical technique.

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