Living Cell Analysis Using Optical Methods

2014 ◽  
pp. 333-352
Author(s):  
Tuan Vo-Dinh
Keyword(s):  
Living Cell ◽  
Optical Methods ◽  
Cell Analysis

scholarly journals Impedance-Based Living Cell Analysis for Clinical Diagnosis of Type I Allergy

Sensors ◽  
2017 ◽  
Vol 17 (11) ◽  
pp. 2503 ◽  
Author(s):  
Reiko Irifuku ◽  
Yuhki Yanase ◽  
Tomoko Kawaguchi ◽  
Kaori Ishii ◽  
Shunsuke Takahagi ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Living Cell ◽  
Type I ◽  
Cell Analysis ◽  
Type I Allergy

Nucleic Acid Aptamers for Living Cell Analysis

2014 ◽  
Vol 7 (1) ◽  
pp. 405-426 ◽  
Author(s):  
Xiangling Xiong ◽  
Yifan Lv ◽  
Tao Chen ◽  
Xiaobing Zhang ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Living Cell ◽  
Cell Analysis ◽  
Nucleic Acid Aptamers

Parameter-optimized digital holographic microscope for high-resolution living-cell analysis

2004 ◽  
Vol 43 (36) ◽  
pp. 6536 ◽  
Author(s):  
Daniel Carl ◽  
Björn Kemper ◽  
Günther Wernicke ◽  
Gert von Bally
Keyword(s):  
High Resolution ◽  
Living Cell ◽  
Cell Analysis

OPTICALLY TRACKING THE MOTION OF MICROBEADS TO STUDY PHYSICAL BEHAVIORS OF THE LIVING CELL IN RESPONSE TO TRANSIENT STRETCH OR COMPRESSION

2011 ◽  
Vol 04 (02) ◽  
pp. 143-150
Author(s):  
LINHONG DENG ◽  
XUEMEI JIANG ◽  
CHENG CHEN ◽  
AIJING SONG ◽  
FENG LIN
Keyword(s):  
Cell Mechanics ◽  
Living Cell ◽  
Actin Polymerization ◽  
Traction Force ◽  
Optical Methods ◽  
Cell Stiffness ◽  
Immunofluorescent Staining ◽  
Adherent Cells ◽  
Force Dynamics ◽  
Research Tools

Optical magnetic twisting cytometry and traction force microscopy are two advanced cell mechanics research tools that employ optical methods to track the motion of microbeads that are either bound to the surface or embedded in the substrate underneath the cell. The former measures rheological properties of the cell such as cell stiffness, and the latter measures cell traction force dynamics. Here we describe the principles of these two cell mechanics research tools and an example of using them to study physical behaviors of the living cell in response to transient stretch or compression. We demonstrate that, when subjected to a stretch–unstretch manipulation, both the stiffness and traction force of adherent cells promptly reduced, and then gradually recover up to the level prior to the stretch. Immunofluorescent staining and Western blotting results indicate that the actin cytoskeleton of the cells underwent a corresponding disruption and reassembly process almost in step with the changes of cell mechanics. Interestingly, when subjected to compression, the cells did not show such particular behaviors. Taken together, we conclude that adherent cells are very sensitive to the transient stretch but not transient compression, and the stretch-induced cell response is due to the dynamics of actin polymerization.

scholarly journals Dynein-dependent Movements of the Mitotic Spindle inSaccharomyces cerevisiae Do Not Require Filamentous Actin

2000 ◽  
Vol 11 (3) ◽  
pp. 863-872 ◽  
Author(s):  
Richard A. Heil-Chapdelaine ◽  
Nguyen K. Tran ◽  
John A. Cooper
Keyword(s):  
Mitotic Spindle ◽  
Living Cell ◽  
Living Cells ◽  
Cell Populations ◽  
Cell Analysis ◽  
Mitotic Spindles ◽  
Filamentous Actin ◽  
Spindle Positioning ◽  
Latrunculin A ◽  
Two Phases

In budding yeast, the mitotic spindle is positioned in the neck between the mother and the bud so that both cells inherit one nucleus. The movement of the mitotic spindle into the neck can be divided into two phases: (1) Kip3p-dependent movement of the nucleus to the neck and alignment of the short spindle, followed by (2) dynein-dependent movement of the spindle into the neck and oscillation of the elongating spindle within the neck. Actin has been hypothesized to be involved in all these movements. To test this hypothesis, we disrupted the actin cytoskeleton with the use of mutations and latrunculin A (latrunculin). We assayed nuclear segregation in synchronized cell populations and observed spindle movements in individual living cells. In synchronized cell populations, no actin cytoskeletal mutant segregated nuclei as poorly as cells lacking dynein function. Furthermore, nuclei segregated efficiently in latrunculin-treated cells. Individual living cell analysis revealed that the preanaphase spindle was mispositioned and misaligned in latrunculin-treated cells and that astral microtubules were misoriented, confirming a role for filamentous actin in the early, Kip3p-dependent phase of spindle positioning. Surprisingly, mispositioned and misaligned mitotic spindles moved into the neck in the absence of filamentous actin, albeit less efficiently. Finally, dynein-dependent sliding of astral microtubules along the cortex and oscillation of the elongating mitotic spindle in the neck occurred in the absence of filamentous actin.

Impedance based living cell analysis for clinical diagnosis of type I allergy

2017 ◽  
Vol 86 (2) ◽  
pp. e28
Author(s):  
Reiko Irifuku ◽  
Yuhki Yanase ◽  
Tomoko Kawaguchi ◽  
Kaori Ishii ◽  
Takaaki Hiragun ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Living Cell ◽  
Type I ◽  
Cell Analysis ◽  
Type I Allergy
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