Genomic Distribution of Genetic Diversity in Elite Maize Germplasm

2018 ◽  
pp. 75-87 ◽  
Author(s):  
Christine Hainey
Keyword(s):  
Genetic Diversity ◽  
Genomic Distribution ◽  
Maize Germplasm

scholarly journals High-throughput retrotransposon-based genetic diversity of maize germplasm assessment and analysis

2020 ◽  
Vol 47 (3) ◽  
pp. 1589-1603 ◽  
Author(s):  
Marwa Ghonaim ◽  
Ruslan Kalendar ◽  
Hoda Barakat ◽  
Nahla Elsherif ◽  
Naglaa Ashry ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
High Throughput ◽  
Genetic Similarity ◽  
Maize Genome ◽  
Ltr Retrotransposons ◽  
Maize Germplasm ◽  
Phylogeny Analysis ◽  
Generating Method ◽  
Polymerase Chain Reaction Primers ◽  
Hybrid Lines

AbstractMaize is one of the world’s most important crops and a model for grass genome research. Long terminal repeat (LTR) retrotransposons comprise most of the maize genome; their ability to produce new copies makes them efficient high-throughput genetic markers. Inter-retrotransposon-amplified polymorphisms (IRAPs) were used to study the genetic diversity of maize germplasm. Five LTR retrotransposons (Huck, Tekay, Opie, Ji, and Grande) were chosen, based on their large number of copies in the maize genome, whereas polymerase chain reaction primers were designed based on consensus LTR sequences. The LTR primers showed high quality and reproducible DNA fingerprints, with a total of 677 bands including 392 polymorphic bands showing 58% polymorphism between maize hybrid lines. These markers were used to identify genetic similarities among all lines of maize. Analysis of genetic similarity was carried out based on polymorphic amplicon profiles and genetic similarity phylogeny analysis. This diversity was expected to display ecogeographical patterns of variation and local adaptation. The clustering method showed that the varieties were grouped into three clusters differing in ecogeographical origin. Each of these clusters comprised divergent hybrids with convergent characters. The clusters reflected the differences among maize hybrids and were in accordance with their pedigree. The IRAP technique is an efficient high-throughput genetic marker-generating method.

Assessing the genetic diversity of Portuguese maize germplasm using microsatellite markers

Euphytica ◽  
2004 ◽  
Vol 137 (1) ◽  
pp. 63-72 ◽  
Author(s):  
M.C. Vaz Patto ◽  
Z. Satovic ◽  
S. Pêgo ◽  
P. Fevereiro
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Maize Germplasm

scholarly journals Genetic Diversity and Molecular Evolution of Chinese Waxy Maize Germplasm

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66606 ◽  
Author(s):  
Hongjian Zheng ◽  
Hui Wang ◽  
Hua Yang ◽  
Jinhong Wu ◽  
Biao Shi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Evolution ◽  
Maize Germplasm ◽  
Waxy Maize ◽  
Chinese Waxy Maize

scholarly journals Genetic Diversity in CIMMYT Nontemperate Maize Germplasm: Landraces, Open Pollinated Varieties, and Inbred Lines

Crop Science ◽  
2008 ◽  
Vol 48 (2) ◽  
pp. 617-624 ◽  
Author(s):  
M. L. Warburton ◽  
J. C. Reif ◽  
M. Frisch ◽  
M. Bohn ◽  
C. Bedoya ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Inbred Lines ◽  
Maize Germplasm

scholarly journals Sequence polymorphism of the waxy gene in waxy maize accessions and characterization of a new waxy allele

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Meijie Luo ◽  
Yaxing Shi ◽  
Yang Yang ◽  
Yanxin Zhao ◽  
Yunxia Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Economic Value ◽  
Waxy Gene ◽  
Maize Breeding ◽  
Pcr Marker ◽  
Maize Germplasm ◽  
Waxy Maize ◽  
Ssr Analysis ◽  
Different Origins

Abstract Waxy maize has many excellent characteristics in terms of its nutritional and economic value. In recent decades, the waxy maize germplasm has increased dramatically as a result of different selection methods. We collected 200 waxy maize inbred accessions from different origins to study their genetic diversity and phylogenetic relationships, and to identify new waxy mutations. A simple sequence repeat (SSR) analysis revealed wide genetic diversity among the 200 waxy maize accessions. The maize accessions were clustered into three groups. We sequenced the waxy gene from the first to the 14th exon. Nucleotide variation analysis of 167 waxy maize and 14 flint maize lines revealed some nucleotide differences in the waxy gene among different waxy maize groups, and much narrower nucleotide diversity in waxy maize than in flint maize. In a phylogenetic analysis, waxy maize carrying the same mutation allele clustered together, and waxy maize carrying different mutation alleles distributed in different groups; waxy maize was intermixed with flint maize in each branch, and wx-D7 waxy maize separated significantly from waxy maize lines carrying wx-D10, wx-124 and wx-hAT mutant alleles. The wx-hAT was a new waxy mutation identified in this study. It consisted of a 2286-bp transposon inserted into the middle of exon three of the waxy gene. A PCR marker specific for the wx-hAT allele was developed. These results will be useful for the utilization and preservation of the waxy maize germplasm, and the PCR marker has potential uses in waxy maize breeding programs.

Genetic diversity of maize germplasm lines and implications for breedingMaize streak virusresistant hybrids

2014 ◽  
Vol 31 (2) ◽  
pp. 77-86 ◽  
Author(s):  
Nothando Mafu ◽  
Roobavathie Naidoo ◽  
Pedro Fato ◽  
Jedidah Danson ◽  
John Derera ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Maize Germplasm

scholarly journals Genetic Diversity of Local Maize Germplasm of Tana Toraja South Sulawesi Using SSR (Simple Sequence Repeat) Markers

2018 ◽  
Vol 2 (3) ◽  
pp. 144
Author(s):  
Ramlah Ramlah ◽  
Isna Rasdianah Aziz ◽  
Cut Muthiadin ◽  
Mashuri Masri ◽  
Muhammad Khalifah Mustami ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Simple Sequence Repeat ◽  
Genetic Similarity ◽  
Agricultural Research ◽  
Similarity Coefficient ◽  
Sequence Repeat ◽  
Maize Germplasm ◽  
South Sulawesi ◽  
Simple Sequence

Plant genetic diversity is an emerging variation in a crop group caused by its genetic factors. Local corn germplasm as a source of plant genes that are able to adapt to the local environment. The purpose of this research is to obtain information on genetic variation of Tana Toraja local maize germ plasm using SSR (Simple Sequence Repeat) marker. This research was conducted at Balitsereal Molecular Biology Laboratory, Agricultural Research Agency in Maros Regency, South Sulawesi. A total of 4 local maize populations were analyzed by laboratory experimental method with observation with NTSYS pc 2.1 program. The results showed that the average number of alleles was 3.72 alleles per locus and the polymorphism rate of 0.53 with the genetic similarity coefficient was in the range of 0.47 to 0.85. 2 main clusters formed in the genetic similarity coefficient 0.47. Klaster I is Local DallePondan and Local Purple. Klaster II is Local Bebo and Kandora. The genetic distance is in the range of 0.15 to 0.74 with an average genetic distance of 0.46. From the data obtained shows that the 4th germplasm of the population of Tana Toraja Local maize diteleti has a very informative level of genetic diversity. Genetic diversity of local maize germplasm of Tana Toraja, can be used as a source of genes in the assembly of improved varieties in the future.

Genetic diversity of maize germplasm assessed by retrotransposon-based markers

2014 ◽  
Vol 35 (12-13) ◽  
pp. 1921-1927 ◽  
Author(s):  
Betty Cristiane Kuhn ◽  
Ignacio López-Ribera ◽  
Maria de Fátima Pires da Silva Machado ◽  
Carlos M. Vicient
Keyword(s):  
Genetic Diversity ◽  
Maize Germplasm

Tropical maize germplasm: what can we say about its genetic diversity in the light of molecular markers?

2005 ◽  
Vol 111 (7) ◽  
pp. 1288-1299 ◽  
Author(s):  
P. R. Laborda ◽  
K. M. Oliveira ◽  
A. A. F. Garcia ◽  
M. E. A. G. Z. Paterniani ◽  
A. P. de Souza
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Tropical Maize ◽  
Maize Germplasm
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